2000
Cytoprotection of Human Umbilical Vein Endothelial Cells Against Apoptosis and CTL-Mediated Lysis Provided by Caspase-Resistant Bcl-2 Without Alterations in Growth or Activation Responses
Zheng L, Dengler T, Kluger M, Madge L, Schechner J, Maher S, Pober J, Bothwell A. Cytoprotection of Human Umbilical Vein Endothelial Cells Against Apoptosis and CTL-Mediated Lysis Provided by Caspase-Resistant Bcl-2 Without Alterations in Growth or Activation Responses. The Journal Of Immunology 2000, 164: 4665-4671. PMID: 10779771, DOI: 10.4049/jimmunol.164.9.4665.Peer-Reviewed Original ResearchMeSH KeywordsApoptosisCaspasesCell DivisionCell Line, TransformedCells, CulturedCulture Media, ConditionedCytotoxicity, ImmunologicEndothelial Growth FactorsEndothelium, VascularGenetic VectorsGreen Fluorescent ProteinsHumansLuminescent ProteinsProto-Oncogene Proteins c-bcl-2RetroviridaeT-Lymphocytes, CytotoxicTransduction, GeneticTransfectionUmbilical VeinsConceptsGraft endothelial cellsAllograft rejectionBcl-2Endothelial cellsAcute allograft rejectionClass I MHC moleculesNF-kappaB activationHuman umbilical vein endothelial cellsI MHC moleculesUmbilical vein endothelial cellsHost CTLVein endothelial cellsEndothelial injuryAnti-apoptotic gene Bcl-2MHC moleculesGene Bcl-2Induction of apoptosisBcl-2-transduced cellsClass IActivation responseApoptotic effectsCTLHUVECTNFGrowth factor withdrawal
1993
HUMAN CD4+ T CELLS PROLIFERATE TO HLA-DR+ ALLOGENEIC VASCULAR ENDOTHELIUM
SAVAGE C, HUGHES C, MCINTYRE B, PICARD J, POBER J. HUMAN CD4+ T CELLS PROLIFERATE TO HLA-DR+ ALLOGENEIC VASCULAR ENDOTHELIUM. Transplantation 1993, 56: 128-134. PMID: 7687392, DOI: 10.1097/00007890-199307000-00024.Peer-Reviewed Original ResearchMeSH KeywordsAntibodies, MonoclonalAntigens, CDAntigens, Differentiation, T-LymphocyteCD2 AntigensCD4 AntigensCD58 AntigensCells, CulturedEndothelium, VascularFlow CytometryHistocompatibility Antigens Class IHLA-DR AntigensHumansInterferon-gammaLymphocyte ActivationMembrane GlycoproteinsPolymerase Chain ReactionReceptors, ImmunologicRecombinant ProteinsT-Lymphocyte SubsetsTransplantation, HomologousUmbilical VeinsConceptsHLA-DR expressing cellsT cellsHLA-DRPolymerase chain reactionEC culturesAllogeneic class II MHC moleculesMHC moleculesLFA-1 beta chainLFA-1Peripheral blood CD4VLA-4 alphaMHC class II moleculesT cell responsesClass II MHC moleculesT cell surface moleculesT cell proliferationClass I MHC moleculesExpressing cellsClass II moleculesBeta chainII MHC moleculesDe novo expressionI MHC moleculesAllogeneic proliferationBlood CD4Four different classes of inhibitors of receptor-mediated endocytosis decrease tumor necrosis factor-induced gene expression in human endothelial cells.
Bradley JR, Johnson DR, Pober JS. Four different classes of inhibitors of receptor-mediated endocytosis decrease tumor necrosis factor-induced gene expression in human endothelial cells. The Journal Of Immunology 1993, 150: 5544-55. PMID: 8390537, DOI: 10.4049/jimmunol.150.12.5544.Peer-Reviewed Original ResearchConceptsIFN-gamma-mediated inductionELAM-1Human endothelial cellsEndothelial cellsMHC moleculesReceptor-mediated endocytosisCytokine-inducible moleculesAnti-inflammatory therapyIL-1-induced increaseClass II MHC moleculesICAM-1 expressionClass I MHC moleculesVCAM-1 expressionCultured human endothelial cellsII MHC moleculesI MHC moleculesNew potential targetsICAM-1VCAM-1Levels of mRNADensity lipoproteinExposure of cellsTNFGene expressionTNF induction
1991
Tumor necrosis factor induction of endothelial cell surface antigens is independent of protein kinase C activation or inactivation. Studies with phorbol myristate acetate and staurosporine.
Ritchie AJ, Johnson DR, Ewenstein BM, Pober JS. Tumor necrosis factor induction of endothelial cell surface antigens is independent of protein kinase C activation or inactivation. Studies with phorbol myristate acetate and staurosporine. The Journal Of Immunology 1991, 146: 3056-62. PMID: 1707932, DOI: 10.4049/jimmunol.146.9.3056.Peer-Reviewed Original ResearchMeSH Keywords1-(5-Isoquinolinesulfonyl)-2-MethylpiperazineAlkaloidsCell Adhesion MoleculesCell CompartmentationE-SelectinEndothelium, VascularEnzyme ActivationHistocompatibility Antigens Class IHumansIn Vitro TechniquesIntercellular Adhesion Molecule-1IsoquinolinesPhosphoproteinsPiperazinesProtein BiosynthesisProtein Kinase CRNA, MessengerSignal TransductionStaurosporineTetradecanoylphorbol AcetateTumor Necrosis Factor-alphaConceptsAdhesion molecule-1Intercellular adhesion molecule-1Molecule-1Endothelial leukocyte adhesion molecule-1 expressionProtein kinase CAdhesion molecule-1 expressionEndothelial leukocyte adhesion molecule-1Leukocyte adhesion molecule-1Surface Ag expressionMolecule-1 expressionClass I MHC moleculesClass I MHCEndothelial cell surface antigensAction of TNFI MHC moleculesCell surface antigensNecrosis factor inductionMechanism of actionPhorbol myristate acetatePKC activationTNF effectsI MHCSurface antigenProtein kinase C activationActivation of PKC
1990
IFN-gamma enhances endothelial activation induced by tumor necrosis factor but not IL-1.
Doukas J, Pober JS. IFN-gamma enhances endothelial activation induced by tumor necrosis factor but not IL-1. The Journal Of Immunology 1990, 145: 1727-33. PMID: 1697308, DOI: 10.4049/jimmunol.145.6.1727.Peer-Reviewed Original ResearchMeSH KeywordsBlotting, NorthernCell Adhesion MoleculesDrug SynergismE-SelectinEndothelium, VascularFluorescent Antibody TechniqueGene ExpressionHistocompatibility Antigens Class IHumansIn Vitro TechniquesIntercellular Adhesion Molecule-1Interferon-gammaInterleukin-1Recombinant ProteinsRNA, MessengerTumor Necrosis Factor-alphaConceptsEndothelial leukocyte adhesion molecule-1ELAM-1 expressionIL-1 betaIFN-gammaIL-1Endothelial cellsEC activationIL-1 beta responseIntercellular adhesion molecule-1 expressionAdhesion molecule-1 expressionMHC Ag expressionLeukocyte adhesion molecule-1Treatment of ECICAM-1 expressionMolecule-1 expressionClass I MHC moleculesTumor necrosis factorAdhesion molecule-1ICAM-1 mRNAClass I MHCI MHC moleculesIFN-gamma effectELAM-1 mRNACultured endothelial cellsIncrease TNF