Mapping the chromatin and RNA expression landscapes of human macrophages to interrogate the function of disease-associated genetic polymorphisms
Martins A, Narayanan M, Fixsen B, Tsang J. Mapping the chromatin and RNA expression landscapes of human macrophages to interrogate the function of disease-associated genetic polymorphisms. The Journal Of Immunology 2017, 198: 59.15-59.15. DOI: 10.4049/jimmunol.198.supp.59.15.Peer-Reviewed Original ResearchSingle-nucleotide polymorphismsRegulatory elementsDisease-associated genetic polymorphismsGenome-wide association studiesGene expressionRegulatory element activityIdentified many single-nucleotide polymorphismsNon-coding regionsCondition-dependent activityHistone 3 lysineModulate gene expressionResponse to diverse stimuliMeasure gene expressionChromatin stateAssociation studiesTranscriptional networksGene modulesExpression landscapeFunctional enrichmentElement activityDiverse stimuliPromoter/enhancer activityGenetic polymorphismsDevelopment of diseaseChromatinEnvironment Tunes Propagation of Cell-to-Cell Variation in the Human Macrophage Gene Network
Martins A, Narayanan M, Prüstel T, Fixsen B, Park K, Gottschalk R, Lu Y, Andrews-Pfannkoch C, Lau W, Wendelsdorf K, Tsang J. Environment Tunes Propagation of Cell-to-Cell Variation in the Human Macrophage Gene Network. Cell Systems 2017, 4: 379-392.e12. PMID: 28365150, PMCID: PMC8392141, DOI: 10.1016/j.cels.2017.03.002.Peer-Reviewed Original ResearchConceptsGene networksCellular adaptationCell variationSingle-cell transcriptomic studiesGene-gene correlationsUnderlying regulatory mechanismsDegree of phosphorylationPhenotypic diversityTranscriptomic studiesEnvironmental adaptationMultiple molecular parametersGene expressionRegulatory mechanismsCellular heterogeneityDistinct environmentsSingle cellsHuman macrophagesQuantitative tuningCell populationsNatural perturbationsGenesDifferent environmentsSuch variationCellsStochastic simulation analysis