2002
Inducible Gene Expression Using an Autoregulatory, Tetracycline‐Controlled System
Shockett P, Schatz D. Inducible Gene Expression Using an Autoregulatory, Tetracycline‐Controlled System. Current Protocols In Molecular Biology 2002, 60: 16.14.1-16.14.9. PMID: 18265300, DOI: 10.1002/0471142727.mb1614s60.Peer-Reviewed Original ResearchConceptsInducible gene expressionSelectable markerGene expressionSecond selectable markerCell linesFibroblast cell lineTransient transfectionGene protein expressionResultant clonesStable linesMarker plasmidPlasmidProtein expressionAdherent cellsTransactivatorExpressionTransfectionCellsTargetGenesSupport protocolClonesLinesMarkersAutoregulatory
1995
A modified tetracycline-regulated system provides autoregulatory, inducible gene expression in cultured cells and transgenic mice.
Shockett P, Difilippantonio M, Hellman N, Schatz DG. A modified tetracycline-regulated system provides autoregulatory, inducible gene expression in cultured cells and transgenic mice. Proceedings Of The National Academy Of Sciences Of The United States Of America 1995, 92: 6522-6526. PMID: 7604026, PMCID: PMC41550, DOI: 10.1073/pnas.92.14.6522.Peer-Reviewed Original ResearchMeSH Keywords3T3 CellsAnimalsBlotting, WesternCells, CulturedDNA NucleotidyltransferasesGene Expression RegulationHerpes Simplex Virus Protein Vmw65MiceMice, TransgenicOpen Reading FramesPlasmidsRecombinant Fusion ProteinsRepressor ProteinsRestriction MappingRNA, MessengerSequence DeletionTetracyclineTrans-ActivatorsTransfectionVDJ RecombinasesConceptsInducible gene expressionGene expressionTetracycline-regulated gene expressionTranscriptional activation domainCultured cellsTetracycline-regulated systemTransgenic miceExpression of tTAAutoregulatory systemActivation domainTTA geneInducible promoterTetracycline repressorInducible expressionFusion proteinTransactivator proteinConstitutive expressionTransgenic animalsGene 1Induced levelsRecombination activityMost tissuesConstitutive systemProteinCell lines