2004
Bcl-2 Transduction Protects Human Endothelial Cell Synthetic Microvessel Grafts from Allogeneic T Cells In Vivo
Zheng L, Gibson TF, Schechner JS, Pober JS, Bothwell AL. Bcl-2 Transduction Protects Human Endothelial Cell Synthetic Microvessel Grafts from Allogeneic T Cells In Vivo. The Journal Of Immunology 2004, 173: 3020-3026. PMID: 15322161, DOI: 10.4049/jimmunol.173.5.3020.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBlood VesselsEndothelial CellsEndothelium, VascularHumansMiceMice, SCIDProstheses and ImplantsProto-Oncogene Proteins c-bcl-2ConceptsEndothelial cellsAllogeneic T cellsT cell responsesHuman immune responseT cell interactionsVascular endothelial cellsHuman T lymphocytesGraft infiltrationAdoptive transferAllograft rejectionImmune surveillanceT cellsT lymphocytesImmune responseImmunological functionsMurine modelAnimal modelsVivo findingsCell responsesMicrovascular bedBcl-2 proteinPathological processesGraftCell interactionsCellsInterferon α but Not Interleukin 12 Activates STAT4 Signaling in Human Vascular Endothelial Cells*
Torpey N, Maher SE, Bothwell AL, Pober JS. Interferon α but Not Interleukin 12 Activates STAT4 Signaling in Human Vascular Endothelial Cells*. Journal Of Biological Chemistry 2004, 279: 26789-26796. PMID: 15087447, DOI: 10.1074/jbc.m401517200.Peer-Reviewed Original ResearchMeSH KeywordsCell Line, TumorCells, CulturedChemokine CCL2DNADNA, ComplementaryDNA-Binding ProteinsDose-Response Relationship, DrugEndothelium, VascularEnzyme-Linked Immunosorbent AssayFlow CytometryHumansImmunoblottingInflammationInterferon-alphaInterleukin-12Oligonucleotide Array Sequence AnalysisPhosphorylationPrecipitin TestsProtein Structure, TertiaryRecombinant ProteinsRepressor ProteinsRetroviridaeReverse Transcriptase Polymerase Chain ReactionRNARNA, MessengerSignal TransductionSTAT4 Transcription FactorSuppressor of Cytokine Signaling 3 ProteinSuppressor of Cytokine Signaling ProteinsTime FactorsTrans-ActivatorsTranscription FactorsUmbilical VeinsUp-RegulationConceptsHuman umbilical vein ECMonocyte chemoattractant protein-1Quantitative RT-PCRVascular endothelial cellsEndothelial cellsChemokine monocyte chemoattractant protein-1Chemoattractant protein-1Cultured human umbilical vein endothelial cellsPro-inflammatory behaviorIFNalpha responseHuman vascular endothelial cellsHuman umbilical vein endothelial cellsUmbilical vein endothelial cellsNeuroblastoma cell linesSuppressor of cytokineVein endothelial cellsEffector cellsInterleukin-12T cellsSTAT4 pathwayInterferon αIL12 receptorCytokinesU3A cellsRT-PCR
2003
Engraftment of a vascularized human skin equivalent
Schechner JS, Crane SK, Wang F, Szeglin AM, Tellides G, Lorber MI, Bothwell AL, Pober JS. Engraftment of a vascularized human skin equivalent. The FASEB Journal 2003, 17: 2250-2256. PMID: 14656987, DOI: 10.1096/fj.03-0257com.Peer-Reviewed Original ResearchConceptsHuman umbilical vein endothelial cellsHuman skin equivalentsAcellular dermis graftsSkin equivalentsBcl-2-transduced human umbilical vein endothelial cellsUmbilical vein endothelial cellsEndothelial cell transplantationVein endothelial cellsCultured human keratinocytesCell transplantationSurvival gene bcl-2Orthotopic implantationSuccessful transplantationDermis graftImmunodeficient miceClinical utilityGraft vascularizationImpaired angiogenesisGene Bcl-2Bcl-2-transduced cellsAcellular dermisEndothelial cellsClinical performanceGraftBcl-2
2002
Porcine Endothelial Cells, Unlike Human Endothelial Cells, Can Be Killed by Human CTL Via Fas Ligand and Cannot Be Protected by Bcl-2
Zheng L, Ben LH, Pober JS, Bothwell AL. Porcine Endothelial Cells, Unlike Human Endothelial Cells, Can Be Killed by Human CTL Via Fas Ligand and Cannot Be Protected by Bcl-2. The Journal Of Immunology 2002, 169: 6850-6855. PMID: 12471117, DOI: 10.4049/jimmunol.169.12.6850.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigens, HeterophileApoptosisCD8-Positive T-LymphocytesCell LineCell Line, TransformedCoculture TechniquesCytotoxicity Tests, ImmunologicCytotoxicity, ImmunologicEndothelium, VascularEpitopes, T-LymphocyteFas Ligand ProteinFas ReceptorGenetic VectorsGranzymesHistocompatibility Antigens Class IHumansImmunity, InnateIsoantigensLigandsMembrane GlycoproteinsPerforinPore Forming Cytotoxic ProteinsProto-Oncogene Proteins c-bcl-2Serine EndopeptidasesSwineT-Lymphocytes, CytotoxicConceptsHuman endothelial cellsPorcine endothelial cellsEndothelial cellsBcl-2MHC class I restrictionClass I restrictionPorcine aortic endothelial cellsTarget cell recognitionAnti-Fas ligand AbAortic endothelial cellsHost CTLAcute rejectionCTL responsesDrug-induced apoptosisHuman CTLPorcine targetsTarget cell typeHuman xenotransplantationFas expressionFas ligandPorcine cellsCTLMajor effectorCell recognitionMajor targetInterferon-γ Augments CD95(APO-1/Fas) and Pro-Caspase-8 Expression and Sensitizes Human Vascular Endothelial Cells to CD95-Mediated Apoptosis
Li JH, Kluger MS, Madge LA, Zheng L, Bothwell AL, Pober JS. Interferon-γ Augments CD95(APO-1/Fas) and Pro-Caspase-8 Expression and Sensitizes Human Vascular Endothelial Cells to CD95-Mediated Apoptosis. American Journal Of Pathology 2002, 161: 1485-1495. PMID: 12368221, PMCID: PMC1867313, DOI: 10.1016/s0002-9440(10)64424-0.Peer-Reviewed Original ResearchAmino Acid Chloromethyl KetonesAntigens, CDApoptosisCaspase 8Caspase 9CaspasesCell DeathCells, CulturedEndothelium, VascularEnzyme InhibitorsEnzyme PrecursorsFas Ligand ProteinFas ReceptorGene Expression RegulationGenetic VectorsHumansInterferon-gammaMembrane GlycoproteinsMicrocirculationRetroviridaeReverse Transcriptase Polymerase Chain ReactionSkinTransfectionUmbilical VeinsCutting Edge: Internalization of Transduced E-Selectin by Cultured Human Endothelial Cells: Comparison of Dermal Microvascular and Umbilical Vein Cells and Identification of a Phosphoserine-Type Di-leucine Motif
Kluger MS, Shiao SL, Bothwell AL, Pober JS. Cutting Edge: Internalization of Transduced E-Selectin by Cultured Human Endothelial Cells: Comparison of Dermal Microvascular and Umbilical Vein Cells and Identification of a Phosphoserine-Type Di-leucine Motif. The Journal Of Immunology 2002, 168: 2091-2095. PMID: 11859093, DOI: 10.4049/jimmunol.168.5.2091.Peer-Reviewed Original ResearchConceptsHuman dermal microvascular endothelial cellsRate of internalizationAspartic acid substitutionSurface protein expressionEndothelial cell behaviorLeucine motifProtein internalizationCytoplasmic regionSurface expression levelsType motifSelectin surface expressionEndothelial cellsMutational analysisHuman endothelial cellsUmbilical vein cellsAcid substitutionsDermal microvascular endothelial cellsCell behaviorCultured human endothelial cellsCytokine inductionExpression levelsVein cellsInternalization processProtein expressionMotif
1996
Porcine endothelial CD86 is a major costimulator of xenogeneic human T cells: cloning, sequencing, and functional expression in human endothelial cells.
Maher SE, Karmann K, Min W, Hughes CC, Pober JS, Bothwell AL. Porcine endothelial CD86 is a major costimulator of xenogeneic human T cells: cloning, sequencing, and functional expression in human endothelial cells. The Journal Of Immunology 1996, 157: 3838-44. PMID: 8892613, DOI: 10.4049/jimmunol.157.9.3838.Peer-Reviewed Original ResearchAbataceptAmino Acid SequenceAnimalsAntigens, CDAntigens, DifferentiationAortaB7-2 AntigenBase SequenceCells, CulturedCHO CellsCloning, MolecularCricetinaeCricetulusCTLA-4 AntigenDNA, ComplementaryEndothelium, VascularHumansImmunoconjugatesInterleukin-2Lymphocyte ActivationMembrane GlycoproteinsMolecular Sequence DataSequence AlignmentSequence Homology, Amino AcidSpecies SpecificitySwineT-LymphocytesTransfectionUmbilical Veins