2004
Qualitatively differential regulation of T cell activation and apoptosis by T cell receptor ζ chain ITAMs and their tyrosine residues
Chae WJ, Lee HK, Han JH, Kim SW, Bothwell AL, Morio T, Lee SK. Qualitatively differential regulation of T cell activation and apoptosis by T cell receptor ζ chain ITAMs and their tyrosine residues. International Immunology 2004, 16: 1225-1236. PMID: 15302845, DOI: 10.1093/intimm/dxh120.Peer-Reviewed Original ResearchConceptsMitogen-activated protein kinaseTCR zeta chainTyrosine phosphorylated proteinsTyrosine residuesZeta chainPhosphorylated proteinsT cell activationCell deathFirst tyrosine residueCell activationInfluence of mutationsActivation-induced cell deathProtein kinaseDistinctive regulationIntracellular signalsDifferential regulationITAMMAPK phosphorylationTCR stimulationStepwise deletionJurkat transfectantsDistinct signalsActivation signalsMutationsResiduesEvidence for a dual pathway of activation in CD43-stimulated Th2 cells: differential requirement for the Lck tyrosine kinase
Fernandez-Cabezudo MJ, Vijayasarathy C, Pflugh DL, Bothwell AL, al-Ramadi BK. Evidence for a dual pathway of activation in CD43-stimulated Th2 cells: differential requirement for the Lck tyrosine kinase. International Immunology 2004, 16: 1215-1223. PMID: 15237112, DOI: 10.1093/intimm/dxh124.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAnimalsAntigens, CDAntigens, Differentiation, T-LymphocyteClone CellsHumansInterleukin-4Lectins, C-TypeLeukosialinLymphocyte ActivationLymphocyte Specific Protein Tyrosine Kinase p56(lck)MiceMultiprotein ComplexesPhosphoproteinsPhosphorylationReceptors, Interleukin-2Shc Signaling Adaptor ProteinsSialoglycoproteinsSignal TransductionSrc Homology 2 Domain-Containing, Transforming Protein 1Th2 CellsConceptsCD43 ligationSLP-76Adaptor protein SLP-76Tyrosine kinaseLck-deficient cellsTyrosine phosphorylated proteinsLck tyrosine kinaseMulti-component complexesT cell biologyNaive splenic T cellsPhosphorylated proteinsMajor protein bandsTyrosine phosphorylationCell biologyPathway componentsDifferential requirementExact functionDifferent effector functionsPhosphorylationT cell moleculesCellular proliferationProtein bandsCell moleculesLckKinaseInterferon α but Not Interleukin 12 Activates STAT4 Signaling in Human Vascular Endothelial Cells*
Torpey N, Maher SE, Bothwell AL, Pober JS. Interferon α but Not Interleukin 12 Activates STAT4 Signaling in Human Vascular Endothelial Cells*. Journal Of Biological Chemistry 2004, 279: 26789-26796. PMID: 15087447, DOI: 10.1074/jbc.m401517200.Peer-Reviewed Original ResearchMeSH KeywordsCell Line, TumorCells, CulturedChemokine CCL2DNADNA, ComplementaryDNA-Binding ProteinsDose-Response Relationship, DrugEndothelium, VascularEnzyme-Linked Immunosorbent AssayFlow CytometryHumansImmunoblottingInflammationInterferon-alphaInterleukin-12Oligonucleotide Array Sequence AnalysisPhosphorylationPrecipitin TestsProtein Structure, TertiaryRecombinant ProteinsRepressor ProteinsRetroviridaeReverse Transcriptase Polymerase Chain ReactionRNARNA, MessengerSignal TransductionSTAT4 Transcription FactorSuppressor of Cytokine Signaling 3 ProteinSuppressor of Cytokine Signaling ProteinsTime FactorsTrans-ActivatorsTranscription FactorsUmbilical VeinsUp-RegulationConceptsHuman umbilical vein ECMonocyte chemoattractant protein-1Quantitative RT-PCRVascular endothelial cellsEndothelial cellsChemokine monocyte chemoattractant protein-1Chemoattractant protein-1Cultured human umbilical vein endothelial cellsPro-inflammatory behaviorIFNalpha responseHuman vascular endothelial cellsHuman umbilical vein endothelial cellsUmbilical vein endothelial cellsNeuroblastoma cell linesSuppressor of cytokineVein endothelial cellsEffector cellsInterleukin-12T cellsSTAT4 pathwayInterferon αIL12 receptorCytokinesU3A cellsRT-PCR
2003
Receptor Activator of NF-κB Ligand Stimulates Recruitment of SHP-1 to the Complex Containing TNFR-Associated Factor 6 That Regulates Osteoclastogenesis
Zhang Z, Jimi E, Bothwell AL. Receptor Activator of NF-κB Ligand Stimulates Recruitment of SHP-1 to the Complex Containing TNFR-Associated Factor 6 That Regulates Osteoclastogenesis. The Journal Of Immunology 2003, 171: 3620-3626. PMID: 14500659, DOI: 10.4049/jimmunol.171.7.3620.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCarrier ProteinsCell DifferentiationCell LineCysteineGenetic VectorsGlycoproteinsIntracellular Signaling Peptides and ProteinsLigandsMacrophagesMembrane GlycoproteinsMiceMice, Inbred C57BLMice, Mutant StrainsNF-kappa BOsteoclastsOsteoprotegerinPhosphatidylinositol 3-KinasesPhosphorylationProtein Phosphatase 1Protein Serine-Threonine KinasesProtein SubunitsProtein TransportProtein Tyrosine Phosphatase, Non-Receptor Type 6Protein Tyrosine PhosphatasesProteinsProto-Oncogene ProteinsProto-Oncogene Proteins c-aktRANK LigandReceptor Activator of Nuclear Factor-kappa BReceptors, Cytoplasmic and NuclearReceptors, Tumor Necrosis FactorRetroviridaeSerineSrc Homology DomainsTNF Receptor-Associated Factor 6Transduction, GeneticTyrosineUp-RegulationConceptsSrc homology 2 domain-containing phosphatase 1Mitogen-activated protein kinaseExpression of SHPProtein kinaseBone marrow macrophagesSrc homology 2 domainTyrosine phosphatase SHP-1P38 mitogen-activated protein kinaseExtracellular signal-regulated kinaseC-Jun N-terminal kinasePhosphatase SHP-1Phosphatidylinositol-3 kinaseMarrow macrophagesAssociation of TRAF6Signal-regulated kinaseN-terminal kinaseMultinuclear osteoclast-like cellsPhosphorylation of AktP85 subunitPhosphatase 1Tyrosine phosphorylationPathways downstreamRANKL-induced phosphorylationRAW264.7 cellsKinase
2002
Immunosuppressive effects of tautomycetin in vivo and in vitro via T cell-specific apoptosis induction
Shim JH, Lee HK, Chang EJ, Chae WJ, Han JH, Han DJ, Morio T, Yang JJ, Bothwell A, Lee SK. Immunosuppressive effects of tautomycetin in vivo and in vitro via T cell-specific apoptosis induction. Proceedings Of The National Academy Of Sciences Of The United States Of America 2002, 99: 10617-10622. PMID: 12149481, PMCID: PMC124991, DOI: 10.1073/pnas.162522099.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntifungal AgentsApoptosisCaspase 3Caspase 9CaspasesCell DivisionCyclosporineFuransHeart TransplantationHeLa CellsHumansImmunosuppressive AgentsJurkat CellsLipidsLymphocyte ActivationMolecular StructurePhosphorylationPoly(ADP-ribose) PolymerasesProto-Oncogene Proteins c-bcl-2RatsRats, Inbred LewRats, WistarReceptor-CD3 Complex, Antigen, T-CellSignal TransductionT-LymphocytesTyrosine