2018
Regulation of human T cell responses by dNP2-ctCTLA-4 inhibits human skin and microvessel graft rejection
Lim S, Kirkiles-Smith NC, Pober JS, Bothwell ALM, Choi JM. Regulation of human T cell responses by dNP2-ctCTLA-4 inhibits human skin and microvessel graft rejection. Biomaterials 2018, 183: 128-138. PMID: 30165256, PMCID: PMC6141312, DOI: 10.1016/j.biomaterials.2018.08.049.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCD4-Positive T-LymphocytesCD8-Positive T-LymphocytesCell ProliferationCell-Penetrating PeptidesCTLA-4 AntigenCytokinesEndothelial CellsFemaleGraft RejectionHuman Umbilical Vein Endothelial CellsHumansLymphocyte ActivationMice, Inbred BALB CMice, KnockoutMice, SCIDMicrovesselsReceptors, ChemokineSkinSkin TransplantationT-LymphocytesConceptsT cell responsesHuman T cell responsesT cell infiltrationHuman T cellsT cellsCell responsesGraft rejectionCell infiltrationSCID/beige miceCell-permeable peptideBlood cytokine levelsT cell alloresponsesCD8 T cellsChemokine receptor expressionGranzyme B expressionAlloreactive T cellsSignificant side effectsDouble knockout miceHuman T cell activationBcl-2-transduced human umbilical vein endothelial cellsT cell activationHuman umbilical vein endothelial cellsUmbilical vein endothelial cellsSystemic immunosuppressantsAllograft rejection
2010
An Implantable Vascularized Protein Gel Construct That Supports Human Fetal Hepatoblast Survival and Infection by Hepatitis C Virus in Mice
Harding MJ, Lepus CM, Gibson TF, Shepherd BR, Gerber SA, Graham M, Paturzo FX, Rahner C, Madri JA, Bothwell AL, Lindenbach BD, Pober JS. An Implantable Vascularized Protein Gel Construct That Supports Human Fetal Hepatoblast Survival and Infection by Hepatitis C Virus in Mice. PLOS ONE 2010, 5: e9987. PMID: 20376322, PMCID: PMC2848675, DOI: 10.1371/journal.pone.0009987.Peer-Reviewed Original ResearchConceptsHepatitis C virusHuman fetal hepatoblastsSmall animal modelsC virusAnimal modelsAccessible small animal modelsHuh-7.5 hepatoma cellsRobust small animal modelHuman hepatocyte engraftmentHuman albumin levelsBcl-2-transduced human umbilical vein endothelial cellsHuman umbilical vein endothelial cellsHepatocyte growth factorUmbilical vein endothelial cellsHCV infectionVein endothelial cellsAlbumin levelsHepatocyte engraftmentBeige miceImmunodeficient miceHistological appearanceImmunoelectron microscopic analysisMRNA expressionViral adsorptionHepatic epithelial cells
2003
Engraftment of a vascularized human skin equivalent
Schechner JS, Crane SK, Wang F, Szeglin AM, Tellides G, Lorber MI, Bothwell AL, Pober JS. Engraftment of a vascularized human skin equivalent. The FASEB Journal 2003, 17: 2250-2256. PMID: 14656987, DOI: 10.1096/fj.03-0257com.Peer-Reviewed Original ResearchConceptsHuman umbilical vein endothelial cellsHuman skin equivalentsAcellular dermis graftsSkin equivalentsBcl-2-transduced human umbilical vein endothelial cellsUmbilical vein endothelial cellsEndothelial cell transplantationVein endothelial cellsCultured human keratinocytesCell transplantationSurvival gene bcl-2Orthotopic implantationSuccessful transplantationDermis graftImmunodeficient miceClinical utilityGraft vascularizationImpaired angiogenesisGene Bcl-2Bcl-2-transduced cellsAcellular dermisEndothelial cellsClinical performanceGraftBcl-2