2019
Low ambient humidity impairs barrier function and innate resistance against influenza infection
Kudo E, Song E, Yockey LJ, Rakib T, Wong PW, Homer RJ, Iwasaki A. Low ambient humidity impairs barrier function and innate resistance against influenza infection. Proceedings Of The National Academy Of Sciences Of The United States Of America 2019, 116: 10905-10910. PMID: 31085641, PMCID: PMC6561219, DOI: 10.1073/pnas.1902840116.Peer-Reviewed Original ResearchConceptsInfluenza infectionImpair barrier functionImpairs host defenseSeasonal influenza virusesInfluenza virus infectionLungs of miceImpairs mucociliary clearanceTissue repairInduction of IFNInnate antiviral defenseViral burdenMucociliary clearanceDisease outcomeRespiratory challengeVirus infectionSevere diseaseViral infectionCongenic miceHost responseViral transmissionHost defenseSingle-cell RNA sequencingInnate resistanceDisease pathologyInfluenza virus
2016
Exploiting Mucosal Immunity for Antiviral Vaccines
Iwasaki A. Exploiting Mucosal Immunity for Antiviral Vaccines. Annual Review Of Immunology 2016, 34: 575-608. PMID: 27168245, DOI: 10.1146/annurev-immunol-032414-112315.Peer-Reviewed Original ResearchConceptsMucosal immunityHuman immunodeficiency virusEffective immune protectionHost immune responseHerpes simplex virusImmunodeficiency virusMucosal vaccinesImmune protectionSuccessful vaccineImmune responseSimplex virusAntiviral vaccinesMucosal surfacesVaccine developmentVaccine designInfluenza virusFirst lineVaccineViral pathogensImmunityViral diseasesVirusDangerous pathogensPathogensDisease
2014
Innate immunity to influenza virus infection
Iwasaki A, Pillai PS. Innate immunity to influenza virus infection. Nature Reviews Immunology 2014, 14: 315-328. PMID: 24762827, PMCID: PMC4104278, DOI: 10.1038/nri3665.Peer-Reviewed Original ResearchConceptsInfluenza virus infectionToll-like receptor 7T cell responsesVirus infectionInterferon-stimulated genesIL-1βNLRP3 inflammasomeViral challengeB cellsCell responsesHigh-dose viral challengeInfluenza virusAntiviral B cellsMultiple pattern recognition receptorsPlasmacytoid dendritic cellsAdaptive immune responsesInfected cellsRetinoic acid-inducible gene IAirway epithelial cellsAcid-inducible gene IPattern recognition receptorsInfluenza virus-infected cellsVirus-infected cellsAntiviral defense genesDendritic cells
2013
Innate immunity
Iwasaki A, Peiris M. Innate immunity. 2013, 267-282. DOI: 10.1002/9781118636817.ch17.Peer-Reviewed Original ResearchToll-like receptorsNOD-like receptorsImmune responseVirus infectionInfluenza virusHundreds of IFNProtective host responseInfluenza virus infectionAdaptive immune responsesInnate immune responseType I IFNInfluenza virus replicationInnate immune systemDendritic cellsNK cellsInfluenza infectionIL-1βInnate sensorsAdaptive immunityLike receptorsDetrimental pathologyI IFNAlveolar macrophagesHost responseImmune systemIL-1R signaling in dendritic cells replaces pattern-recognition receptors in promoting CD8+ T cell responses to influenza A virus
Pang IK, Ichinohe T, Iwasaki A. IL-1R signaling in dendritic cells replaces pattern-recognition receptors in promoting CD8+ T cell responses to influenza A virus. Nature Immunology 2013, 14: 246-253. PMID: 23314004, PMCID: PMC3577947, DOI: 10.1038/ni.2514.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCD8-Positive T-LymphocytesCell DifferentiationCell MovementDendritic CellsInfluenza A virusInterleukin-1Lymphocyte ActivationMembrane GlycoproteinsMembrane ProteinsMiceMice, Inbred C57BLMice, KnockoutMyeloid Differentiation Factor 88Nerve Tissue ProteinsOrthomyxoviridae InfectionsReceptors, CCR7Receptors, Cell SurfaceReceptors, Interleukin-1Receptors, Pattern RecognitionSignal TransductionToll-Like Receptor 7
2010
Inflammasomes as mediators of immunity against influenza virus
Pang IK, Iwasaki A. Inflammasomes as mediators of immunity against influenza virus. Trends In Immunology 2010, 32: 34-41. PMID: 21147034, PMCID: PMC3017631, DOI: 10.1016/j.it.2010.11.004.Peer-Reviewed Original ResearchConceptsToll-like receptor 7Influenza virusMediators of immunityInfluenza virus infectionHuman respiratory infectionsRetinoic acid-inducible gene IAcid-inducible gene IInducible gene IInnate immune systemAdaptive immune defenseEndosomal recognitionInnate detectionRespiratory infectionsReceptor 7Virus infectionCytosolic recognitionInflammasome activationLike receptorsImmune systemImmune defenseMajor causeGene IVirusSuch activationMultiple mechanismsInfluenza virus activates inflammasomes via its intracellular M2 ion channel
Ichinohe T, Pang IK, Iwasaki A. Influenza virus activates inflammasomes via its intracellular M2 ion channel. Nature Immunology 2010, 11: 404-410. PMID: 20383149, PMCID: PMC2857582, DOI: 10.1038/ni.1861.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCarrier ProteinsCells, CulturedCytokinesDendritic CellsGenetic EngineeringGolgi ApparatusHydrogen-Ion ConcentrationIon ChannelsMacrophagesMembrane GlycoproteinsMiceMice, Inbred C57BLMice, KnockoutMonensinNLR Family, Pyrin Domain-Containing 3 ProteinOncogene Proteins, ViralOrthomyxoviridaeOrthomyxoviridae InfectionsPotassium ChlorideProtein TransportProtonsSequence DeletionToll-Like Receptor 7Viral Matrix ProteinsVirus Replication
2009
Inflammasome recognition of influenza virus is essential for adaptive immune responses
Ichinohe T, Lee HK, Ogura Y, Flavell R, Iwasaki A. Inflammasome recognition of influenza virus is essential for adaptive immune responses. Journal Of Experimental Medicine 2009, 206: 79-87. PMID: 19139171, PMCID: PMC2626661, DOI: 10.1084/jem.20081667.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibody FormationApoptosis Regulatory ProteinsCalcium-Binding ProteinsCARD Signaling Adaptor ProteinsCarrier ProteinsCaspase 1CD4-Positive T-LymphocytesCD8-Positive T-LymphocytesCell MovementCytoskeletal ProteinsDendritic CellsImmunity, CellularImmunity, InnateImmunoglobulin IsotypesInterleukin-1betaLungMacrophages, AlveolarMiceMice, Inbred C57BLMice, KnockoutMultiprotein ComplexesNasal Lavage FluidNLR Family, Pyrin Domain-Containing 3 ProteinOrthomyxoviridaeOrthomyxoviridae InfectionsReceptors, Interleukin-1Survival AnalysisConceptsInfluenza virus infectionNOD-like receptorsInfluenza virusVirus infectionAdaptive immunityInflammasome activationRetinoic acid-inducible gene I.CD8 T cell responsesCaspase-1Influenza virus resultsMucosal IgA secretionProtective antiviral immunitySystemic IgG responseCD4 T cellsT cell responsesAdaptive immune responsesType I interferonInnate immune systemRespiratory infectionsIgG responsesProtective immunityTLR signalsIgA secretionReceptor 7T cells
2008
Innate sensors of influenza virus: clues to developing better intranasal vaccines
Ichinohe T, Iwasaki A, Hasegawa H. Innate sensors of influenza virus: clues to developing better intranasal vaccines. Expert Review Of Vaccines 2008, 7: 1435-1445. PMID: 18980544, PMCID: PMC2724183, DOI: 10.1586/14760584.7.9.1435.Peer-Reviewed Original ResearchConceptsInfluenza vaccineInnate sensorsVirus infectionImmune systemInfluenza virusIntranasal influenza vaccineVariant virus infectionNatural infectionEffective influenza vaccinesInfluenza virus infectionToll-like receptorsRetinoic acid-inducible geneNOD-like receptorsInnate immune systemPattern recognition receptorsAdaptive immune systemAcid-inducible geneParenteral immunizationIntranasal vaccineMucosal immunitySystemic immunityInactivated vaccinesRespiratory tractAdaptive immunityLike receptors
1999
Epitope-specific cytotoxic T lymphocyte induction by minigene DNA immunization
Iwasaki A, Dela Cruz C, Young A, Barber B. Epitope-specific cytotoxic T lymphocyte induction by minigene DNA immunization. Vaccine 1999, 17: 2081-2088. PMID: 10217610, DOI: 10.1016/s0264-410x(98)00411-3.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodies, ViralAntigen PresentationBiolisticsCytokinesEpitopes, T-LymphocyteFemaleGenes, ViralHistocompatibility Antigens Class IInjections, IntramuscularMiceMice, Inbred StrainsNucleocapsid ProteinsNucleoproteinsOrthomyxoviridaeOrthomyxoviridae InfectionsPeptide FragmentsPlasmidsProtein Sorting SignalsRecombinant ProteinsRNA-Binding ProteinsT-Lymphocytes, CytotoxicTumor Cells, CulturedVaccines, DNAViral Core ProteinsConceptsCTL responsesCytotoxic T lymphocyte inductionEpitope-specific CTL responsesT lymphocyte responsesT lymphocyte inductionPlasmid DNA vaccineFull-length antigenLymphocyte responsesIL-12Lymphocyte inductionAntibody responseDNA immunizationDNA vaccineGene gun bombardmentPotent antibodiesGM-CSFCytokine combinationsNeedle injectionInfluenza virusSkeletal muscleSkin epidermisNucleoproteinMinimal effectResponseImmunization