2012
Gel-free multiplexed reduced representation bisulfite sequencing for large-scale DNA methylation profiling
Boyle P, Clement K, Gu H, Smith ZD, Ziller M, Fostel JL, Holmes L, Meldrim J, Kelley F, Gnirke A, Meissner A. Gel-free multiplexed reduced representation bisulfite sequencing for large-scale DNA methylation profiling. Genome Biology 2012, 13: r92. PMID: 23034176, PMCID: PMC3491420, DOI: 10.1186/gb-2012-13-10-r92.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCpG IslandsDNA MethylationGenomeHumansMiceMolecular Sequence DataNIH 3T3 CellsSequence Analysis, DNASulfites
2011
Preparation of reduced representation bisulfite sequencing libraries for genome-scale DNA methylation profiling
Gu H, Smith ZD, Bock C, Boyle P, Gnirke A, Meissner A. Preparation of reduced representation bisulfite sequencing libraries for genome-scale DNA methylation profiling. Nature Protocols 2011, 6: 468-481. PMID: 21412275, DOI: 10.1038/nprot.2010.190.Peer-Reviewed Original ResearchMeSH KeywordsCpG IslandsDNA MethylationGene LibraryGenomicsPolymerase Chain ReactionSequence Analysis, DNASulfitesConceptsSingle-nucleotide resolutionGenome-scale DNA methylation profilingCpG-rich DNA fragmentsGenome-wide mappingGenome-wide scaleStandard molecular biology laboratoryMajority of promotersRelevant genomic regionsIllumina Genome AnalyzerNext-generation sequencing approachDNA methylation profilingDNA methylation measurementsRestriction enzyme MspIReduced representationMolecular biology laboratoryGenomic regionsRRBS librariesGenome AnalyzerSequencing librariesMethylation profilingSequencing approachEnzyme MspIAmount of sequencingLow input requirementsGenomic DNA
2009
High-throughput bisulfite sequencing in mammalian genomes
Smith ZD, Gu H, Bock C, Gnirke A, Meissner A. High-throughput bisulfite sequencing in mammalian genomes. Methods 2009, 48: 226-232. PMID: 19442738, PMCID: PMC2864123, DOI: 10.1016/j.ymeth.2009.05.003.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceCpG IslandsDNA MethylationDNA-Cytosine MethylasesGenomic LibraryGenomicsHumansMiceSequence Analysis, DNASulfitesConceptsDNA methylationHigh-throughput bisulfite sequencingCritical epigenetic markCpG-dense regionsGenome-wide distributionArray-based technologiesMammalian genomesEpigenetic marksMammalian developmentGenomic regionsRRBS librariesBisulfite sequencingMethylome profilingSequencing librariesRRBS librarySequence analysisMethylationRestriction digestionSequencingGenomeLibraryRelative coverageMspIProfilingSample throughput