Barbara Ehrlich, PhD
Professor of Pharmacology and of Cellular And Molecular Physiology
Research & Publications
Biography
News
Research Summary
The Laboratory of Molecular Hermeneutics is interested in how cells regulate their intracellular calcium concentration. Cells use changes in calcium as a trigger for many cellular events, including cell growth, secretion, contraction, and neurotransmission. We have focused on one aspect of this process, the release of calcium from intracellular stores. We use calcium imaging combined with electrophysiological, biochemical, and molecular techniques to study the classes of calcium release channels known to exist inside virtually all cells: the InsP3-gated channel, the ryanodine receptor, and polycystin-2. Our first goal is to understand the basic question: how is the function of these channels regulated. Our second goal is to use the answers to the first question to understand the loss of calcium regulation observed in disease states as seen in cells from patients with polycystic kidney disease or leading to drug-induced neuropathy. We hypothesize that these abnormalities in function are consequences, at least in part, of altered intracellular calcium homeostasis and that our studies will lead to suitable treatment regimens.
Specialized Terms: Intracellular calcium regulation
Coauthors
Research Interests
Calcium; Polycystic Kidney Diseases; Pharmacology; Physiology; Drug-Related Side Effects and Adverse Reactions; Hermeneutics
Selected Publications
- Investigating Polycystin‐2‐mediated mitophagy as a stress response through its role in NAFLDBrill A, Kuo I, Ehrlich B. Investigating Polycystin‐2‐mediated mitophagy as a stress response through its role in NAFLD The FASEB Journal 2020, 34: 1-1. DOI: 10.1096/fasebj.2020.34.s1.04144.
- Neuroprotective Effects of Lithium in Chemotherapy‐induced Cognitive ImpairmentsNguyen L, Fischer T, Ehrlich B. Neuroprotective Effects of Lithium in Chemotherapy‐induced Cognitive Impairments The FASEB Journal 2020, 34: 1-1. DOI: 10.1096/fasebj.2020.34.s1.00558.
- Neuronal Calcium Sensor 1 (NCS1) as a Potential Drug Target for Treatment of Wolfram SyndromeFischer T, Nguyen L, Ehrlich B. Neuronal Calcium Sensor 1 (NCS1) as a Potential Drug Target for Treatment of Wolfram Syndrome The FASEB Journal 2020, 34: 1-1. DOI: 10.1096/fasebj.2020.34.s1.00556.
- Mechanisms by Which Glucagon Acutely Stimulates Hepatic Mitochondrial Oxidation and GluconeogenesisPERRY R, WANG Y, BRILL A, PENG L, ZHANG D, DUFOUR S, ZHANG Y, ZHANG X, NOZAKI Y, CLINE G, EHRLICH B, PETERSEN K, SHULMAN G. Mechanisms by Which Glucagon Acutely Stimulates Hepatic Mitochondrial Oxidation and Gluconeogenesis Diabetes 2018, 67 DOI: 10.2337/db18-146-or.
- Role of the mechanosensitive ion channel Piezo1 in Autosomal Dominant Polycystic Kidney Disease (ADPKD)Chebib F, Beyder A, Wang X, Alcaino C, Ehrlich B, Torres V. Role of the mechanosensitive ion channel Piezo1 in Autosomal Dominant Polycystic Kidney Disease (ADPKD) The FASEB Journal 2018, 32: 868.2-868.2. DOI: 10.1096/fasebj.2018.32.1_supplement.868.2.
- Polycystin 2 expression alters calcium‐contraction couplingKuo I, Kwaczala A, Nguyen L, Campbell S, Ehrlich B. Polycystin 2 expression alters calcium‐contraction coupling The FASEB Journal 2015, 29 DOI: 10.1096/fasebj.29.1_supplement.946.1.
- Persistent cAMP Signaling via Vasopressin V2 Receptors Imaged in 3D Cultures Using Lightsheet MicroscopyJiang J, Kuo I, Abi‐Jaoude J, Lemos F, Yang Y, Curci S, Ehrlich B, Hofer A. Persistent cAMP Signaling via Vasopressin V2 Receptors Imaged in 3D Cultures Using Lightsheet Microscopy The FASEB Journal 2015, 29 DOI: 10.1096/fasebj.29.1_supplement.lb632.
- Decreased Polycystin 2 Expression Alters Calcium-Contraction Coupling and Changes Beta-Adrenergic Signaling PathwaysKuo I, Kwaczala A, Nguyen L, Campbell S, Ehrlich B. Decreased Polycystin 2 Expression Alters Calcium-Contraction Coupling and Changes Beta-Adrenergic Signaling Pathways Biophysical Journal 2015, 108: 177a. DOI: 10.1016/j.bpj.2014.11.980.
- Characterization of PC2 Cterm Calcium-Binding Interaction and its Structural ImplicationsYang Y, Keeler C, Kuo I, Lolis E, Hodsdon M, Ehrlich B. Characterization of PC2 Cterm Calcium-Binding Interaction and its Structural Implications Biophysical Journal 2015, 108: 215a. DOI: 10.1016/j.bpj.2014.11.1186.
- The prevention of chemotherapy induced peripheral neuropathy by concurrent treatment with drugs used for bipolar disease: a retrospective chart analysis in human cancer patientsWadia R, Stolar M, Grens C, Ehrlich B, Chao H. The prevention of chemotherapy induced peripheral neuropathy by concurrent treatment with drugs used for bipolar disease: a retrospective chart analysis in human cancer patients Oncotarget 2010, 5: 2-2. DOI: 10.18632/oncotarget.23441.
- Polycystin-2 Contains an Unpaired EF-hand Motif which May Serve as a Ca2+-Sensitive Regulator of Polycystin-2 Channel ActivityCelic A, Petri E, Hodsdon M, Boggon T, Ehrlich B. Polycystin-2 Contains an Unpaired EF-hand Motif which May Serve as a Ca2+-Sensitive Regulator of Polycystin-2 Channel Activity Biophysical Journal 2010, 98: 532a-533a. DOI: 10.1016/j.bpj.2009.12.2890.
- Calcium Induced Conformational Changes In The Cytoplasmic Tail Of Polycystin-2Petri E, Celic A, Boggon T, Ehrlich B. Calcium Induced Conformational Changes In The Cytoplasmic Tail Of Polycystin-2 Biophysical Journal 2009, 96: 119a. DOI: 10.1016/j.bpj.2008.12.526.
- The Amplification Of InsP3R Activity By NCS-1 Is Attenuated By Medications Used In The Treatment Of Bipolar DisorderSchulze C, Olofsson J, Ehrlich B. The Amplification Of InsP3R Activity By NCS-1 Is Attenuated By Medications Used In The Treatment Of Bipolar Disorder Biophysical Journal 2009, 96: 96a. DOI: 10.1016/j.bpj.2008.12.407.
- Regulation Of Inositol 1,4,5-Trisphosphate Receptor Isoforms By O-Linked GlycosylationBimboese P, Gibson C, Schmidt S, Paavola J, Ehrlich B. Regulation Of Inositol 1,4,5-Trisphosphate Receptor Isoforms By O-Linked Glycosylation Biophysical Journal 2009, 96: 97a-98a. DOI: 10.1016/j.bpj.2008.12.412.
- A C-terminal Fragment of Chromogranin B Amplifies Inostiol (1,4,5)-Trisphohsphate Receptor Mediated SignalingSchmidt S, Heidrich F, Mo M, Ehrlich B. A C-terminal Fragment of Chromogranin B Amplifies Inostiol (1,4,5)-Trisphohsphate Receptor Mediated Signaling Biophysical Journal 2009, 96: 97a. DOI: 10.1016/j.bpj.2008.12.409.
- Discrete Proteolysis Of Neuronal Calcium Sensor 1 By μ-calpain Disrupts Calcium BindingBlachford C, Celic A, Petri E, Ehrlich B. Discrete Proteolysis Of Neuronal Calcium Sensor 1 By μ-calpain Disrupts Calcium Binding Biophysical Journal 2009, 96: 121a. DOI: 10.1016/j.bpj.2008.12.534.
- Abstract 1371: NCS-1 is a Potential Therapeutic Target for Avoiding Bradycardia and Optimizing Cancer Chemotherapy with PaclitaxelZhang K, Heidrich F, Ehrlich B. Abstract 1371: NCS-1 is a Potential Therapeutic Target for Avoiding Bradycardia and Optimizing Cancer Chemotherapy with Paclitaxel Circulation 2007, 116 DOI: 10.1161/circ.116.suppl_16.ii_281-c.
- Corrigendum to “Calcium release from ryanodine receptors in the nucleoplasmic reticulum” [Cell Calcium 39 (2006) 65–73]Marius P, Guerra M, Nathanson M, Ehrlich B, Leite M. Corrigendum to “Calcium release from ryanodine receptors in the nucleoplasmic reticulum” [Cell Calcium 39 (2006) 65–73] Cell Calcium 2006, 39: 551. DOI: 10.1016/j.ceca.2006.02.001.
- Ryanodine Receptor Function in InflammationBuck E, Ehrlich B. Ryanodine Receptor Function in Inflammation 2005, 254: 169-178. DOI: 10.1007/0-387-23188-9_17.
- Regulation of Ins(1,4,5)P3 receptor isoforms by endogenous modulatorsThrower E, Hagar R, Ehrlich B. Regulation of Ins(1,4,5)P3 receptor isoforms by endogenous modulators Trends In Pharmacological Sciences 2001, 22: 580-586. PMID: 11698102, DOI: 10.1016/s0165-6147(00)01809-5.
- Human disease: Calcium signaling in polycystic kidney diseaseSomlo S, Ehrlich B. Human disease: Calcium signaling in polycystic kidney disease Current Biology 2001, 11: r356-r360. PMID: 11369247, DOI: 10.1016/s0960-9822(01)00193-2.
- Conservation of localization patterns of IP3 receptor type 1 in cerebellar Purkinje cells across vertebrate speciesKoulen P, Janowitz T, Johnston L, Ehrlich B. Conservation of localization patterns of IP3 receptor type 1 in cerebellar Purkinje cells across vertebrate species Journal Of Neuroscience Research 2000, 61: 493-499. PMID: 10956418, DOI: 10.1002/1097-4547(20000901)61:5<493::aid-jnr3>3.0.co;2-9.
- Regulation of the Type III InsP3 Receptor by InsP3 and ATPHagar R, Ehrlich B. Regulation of the Type III InsP3 Receptor by InsP3 and ATP Biophysical Journal 2000, 79: 271-278. PMID: 10866953, PMCID: PMC1300931, DOI: 10.1016/s0006-3495(00)76289-8.
- Reversible Block of the Calcium Release Channel/Ryanodine Receptor by Protamine, a Heparin AntidoteKoulen P, Ehrlich B. Reversible Block of the Calcium Release Channel/Ryanodine Receptor by Protamine, a Heparin Antidote Molecular Biology Of The Cell 2000, 11: 2213-2219. PMID: 10888663, PMCID: PMC14914, DOI: 10.1091/mbc.11.7.2213.
- Calcium signaling molecules in human cerebellum at midgestation and in ataxiaZecevic N, Milosevic A, Ehrlich B. Calcium signaling molecules in human cerebellum at midgestation and in ataxia Early Human Development 1999, 54: 103-116. PMID: 10213289, DOI: 10.1016/s0378-3782(98)00090-5.
- Type III InsP3 receptor channel stays open in the presence of increased calciumHagar R, Burgstahler A, Nathanson M, Ehrlich B. Type III InsP3 receptor channel stays open in the presence of increased calcium Nature 1998, 396: 81-84. PMID: 9817204, PMCID: PMC2825878, DOI: 10.1038/23954.
- Characterization of the ryanodine receptor/channel of invertebrate muscleQuinn K, Castellani L, Ondrias K, Ehrlich B. Characterization of the ryanodine receptor/channel of invertebrate muscle American Journal Of Physiology 1998, 274: r494-r502. PMID: 9486309, DOI: 10.1152/ajpregu.1998.274.2.r494.
- Inositol 1,4,5-Trisphosphate (InsP3) and Calcium Interact to Increase the Dynamic Range of InsP3 Receptor-dependent Calcium SignalingKaftan E, Ehrlich B, Watras J. Inositol 1,4,5-Trisphosphate (InsP3) and Calcium Interact to Increase the Dynamic Range of InsP3 Receptor-dependent Calcium Signaling The Journal Of General Physiology 1997, 110: 529-538. PMID: 9348325, PMCID: PMC2229389, DOI: 10.1085/jgp.110.5.529.
- Regulation of Intracellular Calcium Release Channel Function by Arachidonic Acid and Leukotriene B4Striggow F, Ehrlich B. Regulation of Intracellular Calcium Release Channel Function by Arachidonic Acid and Leukotriene B4 Biochemical And Biophysical Research Communications 1997, 237: 413-418. PMID: 9268725, DOI: 10.1006/bbrc.1997.7152.
- Methanethiosulfonate Derivatives Inhibit Current through the Ryanodine Receptor/ChannelQuinn K, Ehrlich B. Methanethiosulfonate Derivatives Inhibit Current through the Ryanodine Receptor/Channel The Journal Of General Physiology 1997, 109: 255-264. PMID: 9041453, PMCID: PMC2220055, DOI: 10.1085/jgp.109.2.255.
- Ligand-gated calcium channels inside and outStriggow F, Ehrlich B. Ligand-gated calcium channels inside and out Current Opinion In Cell Biology 1996, 8: 490-495. PMID: 8791458, DOI: 10.1016/s0955-0674(96)80025-1.
- Effects of Rapamycin on Ryanodine Receptor/Ca2+-Release Channels From Cardiac MuscleKaftan E, Marks A, Ehrlich B. Effects of Rapamycin on Ryanodine Receptor/Ca2+-Release Channels From Cardiac Muscle Circulation Research 1996, 78: 990-997. PMID: 8635249, DOI: 10.1161/01.res.78.6.990.
- Functional properties of intracellular calcium-release channelsEhrlich B. Functional properties of intracellular calcium-release channels Current Opinion In Neurobiology 1995, 5: 304-309. PMID: 7580152, DOI: 10.1016/0959-4388(95)80042-5.
- The pharmacology of intracellular Ca2+-release channelsEhrlich B, Kaftan E, Bezprozvannaya S, Bezprozvanny I. The pharmacology of intracellular Ca2+-release channels Trends In Pharmacological Sciences 1994, 15: 145-149. PMID: 7754532, DOI: 10.1016/0165-6147(94)90074-4.
- Stabilization of calcium release channel (ryanodine receptor) function by FK506-binding proteinBrillantes A, Ondrias K, Scott A, Kobrinsky E, Ondriašová E, Moschella M, Jayaraman T, Landers M, Ehrlich B, Marks A. Stabilization of calcium release channel (ryanodine receptor) function by FK506-binding protein Cell 1994, 77: 513-523. PMID: 7514503, DOI: 10.1016/0092-8674(94)90214-3.
- CHAPTER 33 InsP3 Receptor: Functional Properties and RegulationBEZPROZVANNY I, EHRLICH B. CHAPTER 33 InsP3 Receptor: Functional Properties and Regulation 1994, 511-526. DOI: 10.1016/b978-0-12-550640-3.50038-1.
- ATP modulates the function of inositol 1,4,5-trisphosphate-gated channels at two sitesBezprozvanny I, Ehrlich B. ATP modulates the function of inositol 1,4,5-trisphosphate-gated channels at two sites Neuron 1993, 10: 1175-1184. PMID: 7686381, DOI: 10.1016/0896-6273(93)90065-y.
- [31] Planar lipid bilayers on patch pipettes: Bilayer formation and ion channel incorporationEhrlich B. [31] Planar lipid bilayers on patch pipettes: Bilayer formation and ion channel incorporation 1992, 207: 463-470. PMID: 1382197, DOI: 10.1016/0076-6879(92)07033-k.
- Inositol 1,4,5-trisphosphate-gated channels in cerebellum: presence of multiple conductance statesWatras J, Bezprozvanny I, Ehrlich B. Inositol 1,4,5-trisphosphate-gated channels in cerebellum: presence of multiple conductance states Journal Of Neuroscience 1991, 11: 3239-3245. PMID: 1719158, PMCID: PMC6575433, DOI: 10.1523/jneurosci.11-10-03239.1991.
- Bell-shaped calcium-response curves of lns(l,4,5)P3- and calcium-gated channels from endoplasmic reticulum of cerebellumBezprozvanny L, Watras J, Ehrlich B. Bell-shaped calcium-response curves of lns(l,4,5)P3- and calcium-gated channels from endoplasmic reticulum of cerebellum Nature 1991, 351: 751-754. PMID: 1648178, DOI: 10.1038/351751a0.
- Comparison of calcium release from sarcoplasmic reticulum of slow and fast twitch musclesLee Y, Ondrias K, Duhl A, Ehrlich B, Kim D. Comparison of calcium release from sarcoplasmic reticulum of slow and fast twitch muscles The Journal Of Membrane Biology 1991, 122: 155-163. PMID: 1716686, DOI: 10.1007/bf01872638.
- Doxorubicin has biphasic effects on cardiac sarcoplasmic reticulum calcium channels in planar lipid bilayersBorgatta L, Ondrias K, Do Han Kim, Ehrlich B. Doxorubicin has biphasic effects on cardiac sarcoplasmic reticulum calcium channels in planar lipid bilayers Journal Of Molecular And Cellular Cardiology 1990, 22: s29. DOI: 10.1016/0022-2828(90)91394-m.
- Comparison of calcium release channels in reticular membranes from smooth muscle, skeletal muscle and cerebellumWatras J, Adler P, Ondrias K, Ehrlich B. Comparison of calcium release channels in reticular membranes from smooth muscle, skeletal muscle and cerebellum Journal Of Molecular And Cellular Cardiology 1990, 22: s9. DOI: 10.1016/0022-2828(90)91336-6.
- Calcium Channels Incorporated into Planar Lipid Bilayers: Phenomenology, Pharmacology, and PhylogenyEhrlich B, Forte M. Calcium Channels Incorporated into Planar Lipid Bilayers: Phenomenology, Pharmacology, and Phylogeny 1988, 33-41. DOI: 10.1007/978-1-4613-0975-8_3.
- Lithium transport across squid axon membraneEhrlich B, Russell J. Lithium transport across squid axon membrane Brain Research 1984, 311: 141-143. PMID: 6091848, DOI: 10.1016/0006-8993(84)91406-9.
- Voltage-Dependent Calcium Channels from Paramecium Cilia Incorporated into Planar Lipid BilayersEhrlich B, Finkelstein A, Forte M, Kung C. Voltage-Dependent Calcium Channels from Paramecium Cilia Incorporated into Planar Lipid Bilayers Science 1984, 225: 427-428. PMID: 6330895, DOI: 10.1126/science.6330895.
- Lithium concentration in the muscle compartment of manic-depressive patients during lithium therapyEhrlich B, Clausen C, Gosenfeld L, Diamond J. Lithium concentration in the muscle compartment of manic-depressive patients during lithium therapy Journal Of Psychiatric Research 1984, 18: 139-148. PMID: 6747912, DOI: 10.1016/0022-3956(84)90005-0.
- Lithium's inhibition of erythrocyte cation countertransport involves a slow process in the erythrocyteEhrlich B, Diamond J, Fry V, Meier K. Lithium's inhibition of erythrocyte cation countertransport involves a slow process in the erythrocyte The Journal Of Membrane Biology 1983, 75: 233-240. PMID: 6631944, DOI: 10.1007/bf01871954.
- Lithium absorption in tight and leaky segments of intestineDiamond J, Ehrlich B, Morawski S, Santa Ana C, Dordtran J. Lithium absorption in tight and leaky segments of intestine The Journal Of Membrane Biology 1983, 72: 153-159. PMID: 6854620, DOI: 10.1007/bf01870322.
- Choline and PAH transport across blood-CSF barriers: The effect of lithiumEhrlich B, Wright E. Choline and PAH transport across blood-CSF barriers: The effect of lithium Brain Research 1982, 250: 245-250. PMID: 6293641, DOI: 10.1016/0006-8993(82)90418-8.
- Lithium-induced changes in sodium-lithium countertransportEhrlich B, Diamond J, Gosenfeld L. Lithium-induced changes in sodium-lithium countertransport Biochemical Pharmacology 1981, 30: 2539-2543. PMID: 7306206, DOI: 10.1016/0006-2952(81)90580-3.
- Lithium, membranes, and manic-depressive illnessEhrlich B, Diamond J. Lithium, membranes, and manic-depressive illness The Journal Of Membrane Biology 1980, 52: 187-200. PMID: 6991708, DOI: 10.1007/bf01869189.
- Lithium pharmacokinetics: Single-dose experiments and analysis using a physiological modelEhrlich B, Clausen C, Diamond J. Lithium pharmacokinetics: Single-dose experiments and analysis using a physiological model Journal Of Pharmacokinetics And Pharmacodynamics 1980, 8: 439-461. PMID: 7252790, DOI: 10.1007/bf01059545.
- Effects of lithium on blood-brain barrier transport of the neurotransmitter precursors choline, tyrosine and tryptophanEhrlich B, Diamond J, Braun L, Cornford E, Oldendorf W. Effects of lithium on blood-brain barrier transport of the neurotransmitter precursors choline, tyrosine and tryptophan Brain Research 1980, 193: 604-607. PMID: 7388615, DOI: 10.1016/0006-8993(80)90198-5.
- An ultramicro method for analysis of lithium and other biologically important cationsEhrlich B, Diamond J. An ultramicro method for analysis of lithium and other biologically important cations Biochimica Et Biophysica Acta 1978, 543: 264-268. PMID: 728464, DOI: 10.1016/0304-4165(78)90072-7.