2015
Exome sequencing identifies recurrent mutations in NF1 and RASopathy genes in sun-exposed melanomas
Krauthammer M, Kong Y, Bacchiocchi A, Evans P, Pornputtapong N, Wu C, McCusker JP, Ma S, Cheng E, Straub R, Serin M, Bosenberg M, Ariyan S, Narayan D, Sznol M, Kluger HM, Mane S, Schlessinger J, Lifton RP, Halaban R. Exome sequencing identifies recurrent mutations in NF1 and RASopathy genes in sun-exposed melanomas. Nature Genetics 2015, 47: 996-1002. PMID: 26214590, PMCID: PMC4916843, DOI: 10.1038/ng.3361.Peer-Reviewed Original ResearchMeSH KeywordsAntineoplastic AgentsBenzimidazolesDNA Mutational AnalysisDrug Resistance, NeoplasmExomeGenetic Association StudiesGenetic Predisposition to DiseaseHumansInhibitory Concentration 50Kaplan-Meier EstimateLoss of HeterozygosityMaleMelanomaMutation, MissenseNeurofibromin 1Ras ProteinsSequence Analysis, RNASkin NeoplasmsSunlightTumor Cells, Cultured
2009
Genome-wide screen of promoter methylation identifies novel markers in melanoma
Koga Y, Pelizzola M, Cheng E, Krauthammer M, Sznol M, Ariyan S, Narayan D, Molinaro AM, Halaban R, Weissman SM. Genome-wide screen of promoter methylation identifies novel markers in melanoma. Genome Research 2009, 19: 1462-1470. PMID: 19491193, PMCID: PMC2720187, DOI: 10.1101/gr.091447.109.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAdultAgedBiomarkers, TumorCells, CulturedCluster AnalysisCollagenCollagen Type IDNA MethylationFemaleGene Expression ProfilingGenome, HumanGenome-Wide Association StudyHSP20 Heat-Shock ProteinsHumansInfant, NewbornMaleMelanomaMetallothioneinMiddle AgedMolecular ChaperonesNuclear ProteinsNucleoplasminsOligonucleotide Array Sequence AnalysisPhosphoproteinsPromoter Regions, GeneticProteinsReproducibility of ResultsReverse Transcriptase Polymerase Chain ReactionSequence Analysis, DNATumor Cells, CulturedConceptsDifferential gene expressionGene expressionPromoter methylationGenome-wide promoter methylationGenome-wide integrative analysisPromoter CpG contentMethylation markersGenome-wide screenSequencing of bisulfiteTranscription start siteMelanoma cell strainsCell strainsTranscriptional machineryNovel genesEpigenetic modificationsDNA methylationIdentifies novel markersStart siteSnap-frozen tissuesCpG contentAdult melanocytesIntegrative analysisReal-time reverse transcriptase PCRHuman diseasesMethylation
2002
Abnormal Acidification of Melanoma Cells Induces Tyrosinase Retention in the Early Secretory Pathway*
Halaban R, Patton RS, Cheng E, Svedine S, Trombetta ES, Wahl ML, Ariyan S, Hebert DN. Abnormal Acidification of Melanoma Cells Induces Tyrosinase Retention in the Early Secretory Pathway*. Journal Of Biological Chemistry 2002, 277: 14821-14828. PMID: 11812790, DOI: 10.1074/jbc.m111497200.Peer-Reviewed Original Research
2000
Deregulated E2f Transcriptional Activity in Autonomously Growing Melanoma Cells
Halaban R, Cheng E, Smicun Y, Germino J. Deregulated E2f Transcriptional Activity in Autonomously Growing Melanoma Cells. Journal Of Experimental Medicine 2000, 191: 1005-1016. PMID: 10727462, PMCID: PMC2193116, DOI: 10.1084/jem.191.6.1005.Peer-Reviewed Original ResearchMeSH KeywordsCarrier ProteinsCell Cycle ProteinsCell DivisionCells, CulturedCellular SenescenceCulture Media, ConditionedCyclin-Dependent KinasesCyclinsDNA-Binding ProteinsDown-RegulationDrosophila ProteinsE2F Transcription FactorsE2F1 Transcription FactorE2F2 Transcription FactorE2F3 Transcription FactorE2F4 Transcription FactorE2F5 Transcription FactorE2F6 Transcription FactorEnzyme InhibitorsFlavonoidsHumansMelanocytesMelanomaPhosphorylationPiperidinesProtein BindingRetinoblastoma-Binding Protein 1Trans-ActivatorsTranscription Factor DP1Transcription FactorsTumor Cells, CulturedConceptsExternal growth factorsE2F DNAPocket proteinsCyclin-dependent kinase 4Normal melanocytesTranscriptional activityRetinoblastoma tumor suppressor proteinMelanoma cellsMalignant human melanocytesE2F transcription factorsE2F family membersE2F transcriptional activityTumor suppressor proteinGel shift analysisCell cycle progressionForm of pRBGrowth factorContinuous high expressionE2F activityOnly family memberTranscription factorsProtein DNASuppressor proteinFamily membersMolecular basis
1997
Suppression of autocrine cell proliferation and tumorigenesis of human melanoma cells and fibroblast growth factor transformed fibroblasts by a kinase-deficient FGF receptor 1: evidence for the involvement of Src-family kinases
Yayon A, Ma Y, Safran M, Klagsbrun M, Halaban R. Suppression of autocrine cell proliferation and tumorigenesis of human melanoma cells and fibroblast growth factor transformed fibroblasts by a kinase-deficient FGF receptor 1: evidence for the involvement of Src-family kinases. Oncogene 1997, 14: 2999-3009. PMID: 9223663, DOI: 10.1038/sj.onc.1201159.Peer-Reviewed Original ResearchMeSH Keywords3T3 CellsAnimalsCell DivisionCell Line, TransformedFibroblast Growth Factor 2FibroblastsFilaggrin ProteinsGenes, DominantHumansMelanomaMicePhenotypeProtein-Tyrosine KinasesReceptor Protein-Tyrosine KinasesReceptor, Fibroblast Growth Factor, Type 1Receptors, Fibroblast Growth FactorRecombinant ProteinsSrc Homology DomainsTumor Cells, CulturedConceptsSrc family kinasesFGF receptor 1FGF receptorsTyrosyl-phosphorylated proteinsHuman melanoma cellsMelanoma cellsImmune kinase assayIntracellular kinase domainDominant negative mutantActivated FGF receptorCell proliferationMetastatic melanoma cellsMelanoma tumor progressionGrowth factorTransmembrane domainKinase assaysSrc familyKinase domainDownstream targetsReceptor 1Fibroblast growth factorBasic fibroblast growth factorGrowth advantageNormal melanocytesAutocrine activationAberrant retention of tyrosinase in the endoplasmic reticulum mediates accelerated degradation of the enzyme and contributes to the dedifferentiated phenotype of amelanotic melanoma cells
Halaban R, Cheng E, Zhang Y, Moellmann G, Hanlon D, Michalak M, Setaluri V, Hebert D. Aberrant retention of tyrosinase in the endoplasmic reticulum mediates accelerated degradation of the enzyme and contributes to the dedifferentiated phenotype of amelanotic melanoma cells. Proceedings Of The National Academy Of Sciences Of The United States Of America 1997, 94: 6210-6215. PMID: 9177196, PMCID: PMC21028, DOI: 10.1073/pnas.94.12.6210.Peer-Reviewed Original ResearchMeSH KeywordsAdultCalcium-Binding ProteinsCalnexinCalreticulinCell DifferentiationCells, CulturedCoculture TechniquesEndoplasmic ReticulumEnzyme PrecursorsHumansInfant, NewbornKineticsMelanocytesMelanomaMolecular ChaperonesMolecular WeightMonophenol MonooxygenasePhenotypeRibonucleoproteinsSkin NeoplasmsTime FactorsTumor Cells, CulturedConceptsEndoplasmic reticulumNormal melanocytesER chaperone calnexinMelanin synthesisMalignant melanocytesMelanoma cellsChaperone bindingAberrant retentionChaperone calnexinGolgi compartmentSubcellular localizationAmelanotic melanoma cell lineKey enzymeMelanoma cell linesMaturation of tyrosinaseAmelanotic melanoma cellsKinetics of synthesisInhibitor sensitivityDedifferentiated phenotypeProteolytic degradationCell linesProteasome inhibitorsEnzymeProteasomeImmature formsRecognition of activated CSF-1 receptor in breast carcinomas by a tyrosine 723 phosphospecific antibody
Flick M, Sapi E, Perrotta P, Maher M, Halaban R, Carter D, Kacinski B. Recognition of activated CSF-1 receptor in breast carcinomas by a tyrosine 723 phosphospecific antibody. Oncogene 1997, 14: 2553-2561. PMID: 9191055, DOI: 10.1038/sj.onc.1201092.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAntibodiesAntibody SpecificityBreast NeoplasmsCarcinoma in SituCells, CulturedCross ReactionsEpitopesFibroblastsGenes, fmsHumansImmunohistochemistryMacrophagesMiceMice, Inbred BALB CPhosphopeptidesPhosphorylationPhosphotyrosineProto-Oncogene MasReceptor, Macrophage Colony-Stimulating FactorTumor Cells, CulturedConceptsCSF-1RMultiple signal transduction pathwaysC-fms proto-oncogenePhosphorylation state-specific antibodiesSignal transduction pathwaysCSF-1 receptorAnchorage-independent growthImmunoblots of lysatesActivation/phosphorylationEffector proteinsVivo phosphorylationCytoplasmic domainDifferentiation of macrophagesPhosphospecific antibodiesTransduction pathwaysCellular phenotypesInvasive human breast tumoursSpecific phosphopeptidesC-fms oncogeneCSF-1Proto-oncogeneHuman breast carcinomaFirst direct evidenceNormal proliferationFactor receptor
1995
VEGF121, a Vascular Endothelial Growth Factor (VEGF) Isoform Lacking Heparin Binding Ability, Requires Cell-surface Heparan Sulfates for Efficient Binding to the VEGF Receptors of Human Melanoma Cells*
Cohen T, Gitay-Goren H, Sharon R, Shibuya M, Halaban R, Levi B, Neufeld G. VEGF121, a Vascular Endothelial Growth Factor (VEGF) Isoform Lacking Heparin Binding Ability, Requires Cell-surface Heparan Sulfates for Efficient Binding to the VEGF Receptors of Human Melanoma Cells*. Journal Of Biological Chemistry 1995, 270: 11322-11326. PMID: 7744769, DOI: 10.1074/jbc.270.19.11322.Peer-Reviewed Original ResearchMeSH KeywordsAlternative SplicingBinding SitesCell DivisionCell LineCell MembraneCross-Linking ReagentsEndothelial Growth FactorsGene ExpressionGenetic VariationHeparinHeparitin SulfateHumansKineticsLymphokinesMelanomaReceptor Protein-Tyrosine KinasesReceptors, Growth FactorReceptors, MitogenReceptors, Vascular Endothelial Growth FactorRNA, MessengerTumor Cells, CulturedVascular Endothelial Growth Factor AVascular Endothelial Growth FactorsConceptsHuman melanoma cellsVEGF receptorsMelanoma cellsCell surface heparan sulfateVEGF receptor Flt-1VEGF receptor KDR/flkVascular endothelial growth factor isoformsHeparan sulfateReceptor KDR/flkReceptor Flt-1Growth factor isoformsCell surface heparin-like moleculesKDR/FlkVascular endothelial growth factor (VEGF) splice variantsHeparin-like moleculesAddition of heparin
1993
KIT ligand (mast cell growth factor) inhibits the growth of KIT-expressing melanoma cells.
Zakut R, Perlis R, Eliyahu S, Yarden Y, Givol D, Lyman S, Halaban R. KIT ligand (mast cell growth factor) inhibits the growth of KIT-expressing melanoma cells. Oncogene 1993, 8: 2221-9. PMID: 7687762.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceCell DivisionHematopoietic Cell Growth FactorsHumansMelanomaMolecular Sequence DataNeoplasm ProteinsPhosphorylationProtein-Tyrosine KinasesProto-Oncogene MasProto-Oncogene ProteinsProto-Oncogene Proteins c-kitRNA, MessengerRNA, NeoplasmStem Cell FactorTumor Cells, CulturedConceptsMast cell growth factorMelanoma cell linesNormal melanocyte developmentMelanoma cellsAberrant signal transductionMetastatic melanoma cell linesCell linesActivation of KITMelanocyte developmentSignal transductionKIT proto-oncogeneKinase activationKIT mRNAKit ligandNormal melanocytesProto-oncogeneKIT kinaseCell growth factorBiological responsesGrowth factorHuman melanomaMelanocytesCellsKIT expressionNeoplastic melanocytesA transcriptional inhibitor induced in human melanoma cells upon ultraviolet irradiation.
Yang Y, Rutberg S, Luo F, Spratt T, Halaban R, Ferrone S, Ronai Z. A transcriptional inhibitor induced in human melanoma cells upon ultraviolet irradiation. Molecular Cancer Research 1993, 4: 595-602. PMID: 8398900.Peer-Reviewed Original Research
1992
Scatter factor and hepatocyte growth factor: activities, properties, and mechanism.
Bhargava M, Joseph A, Knesel J, Halaban R, Li Y, Pang S, Goldberg I, Setter E, Donovan M, Zarnegar R. Scatter factor and hepatocyte growth factor: activities, properties, and mechanism. Molecular Cancer Research 1992, 3: 11-20. PMID: 1534687.Peer-Reviewed Original ResearchConceptsHepatocyte growth factorScatter factorPartial amino acid sequence dataAmino acid sequence dataAmino acid sequenceFibroblast-derived proteinHuman smooth muscle cellsVascular endothelial cell typesEndothelial cell typesGrowth factorSignificant homologyPosttranslational modificationsSequence dataRapid phosphorylationAcid sequenceBiological activityTyrosine residuesProtein productsCohesive coloniesCommon antigenic determinantsCell typesSpecies-related differencesProteinSerum-derived proteinsHuman hepatocyte growth factor
1988
bFGF as an autocrine growth factor for human melanomas.
Halaban R, Kwon BS, Ghosh S, Delli Bovi P, Baird A. bFGF as an autocrine growth factor for human melanomas. Oncogene Research 1988, 3: 177-86. PMID: 3226725.Peer-Reviewed Original ResearchMeSH KeywordsCell Transformation, NeoplasticFibroblast Growth FactorsHumansMelanocytesMelanomaMitogensTumor Cells, CulturedConceptsBasic fibroblast growth factorNormal human melanocytesBFGF gene transcriptsHuman melanocytesGrowth factorAutocrine growth factorGene transcriptsHuman metastatic melanomaMolecular mechanismsFibroblast growth factorNormal melanocytesCyclic adenosine monophosphateSerum-containing mediumAnti-bFGF antibodyMelanoma cellsAbnormal expressionAdenosine monophosphateHuman melanomaMelanocytesRegular culture mediumDibutyryl cyclic adenosine monophosphateMitogenic activityCulture mediumCellsTranscripts
1987
A melanocyte-specific complementary DNA clone whose expression is inducible by melanotropin and isobutylmethyl xanthine.
Kwon B, Halaban R, Kim G, Usack L, Pomerantz S, Haq A. A melanocyte-specific complementary DNA clone whose expression is inducible by melanotropin and isobutylmethyl xanthine. Molecular Biology & Medicine 1987, 4: 339-55. PMID: 2449595.Peer-Reviewed Original ResearchMeSH Keywords1-Methyl-3-isobutylxanthineAnimalsAntibodies, MonoclonalCatechol OxidaseDNAGene Expression RegulationGlycoproteinsHumansMelaninsMelanocyte-Stimulating HormonesMelanocytesMelanomaMelanoma, ExperimentalMiceMonophenol MonooxygenaseNeoplasm ProteinsPigmentationSpecies SpecificityTheophyllineTumor Cells, CulturedConceptsPmel 17CDNA clonesLambda gt11 cDNA libraryComplementary DNA cloneHuman tyrosinase geneNormal human melanocytesSingle geneDNA clonesCDNA libraryStimulation of humanMRNA speciesTyrosinase geneMurine melanocytesMurine DNAMurine melanoma cellsRestriction fragmentsHuman melanocytesRepresentative clonesGenesClonesImmunological homologyCDNAMelanoma cellsMelanocytesSpecies