Enhanced and prolonged cross‐presentation following endosomal escape of exogenous antigens encapsulated in biodegradable nanoparticles
Shen H, Ackerman AL, Cody V, Giodini A, Hinson ER, Cresswell P, Edelson RL, Saltzman WM, Hanlon DJ. Enhanced and prolonged cross‐presentation following endosomal escape of exogenous antigens encapsulated in biodegradable nanoparticles. Immunology 2005, 117: 78-88. PMID: 16423043, PMCID: PMC1782199, DOI: 10.1111/j.1365-2567.2005.02268.x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigen PresentationBiocompatible MaterialsBiodegradation, EnvironmentalB-LymphocytesCell LineCross-PrimingDendritic CellsEndosomesHistocompatibility Antigens Class IIHumansLactic AcidLymphocyte ActivationMiceMice, Inbred C57BLNanostructuresOvalbuminPolyglycolic AcidPolylactic Acid-Polyglycolic Acid CopolymerPolymersSerum Albumin, BovineT-LymphocytesConceptsBone marrow-derived dendritic cellsMHC class I presentationAntigen-presenting cellsClass I presentationMHC class IExogenous antigensDendritic cellsClass IAntigen deliveryPrimary mouse bone marrow-derived dendritic cellsSoluble antigenMouse bone marrow-derived dendritic cellsMarrow-derived dendritic cellsProfessional antigen-presenting cellsMajor histocompatibility complex class IProtein-based vaccinationT cell responsesClassic MHC class IExogenous antigen presentationHistocompatibility complex class IAntigen-coated latex beadsCell-associated antigensInterleukin-2 secretionComplex class IEfficiency of presentationGeneration of dendritic cells from rabbit bone marrow mononuclear cell cultures supplemented with hGM-CSF and hIL-4
Cody V, Shen H, Shlyankevich M, Tigelaar RE, Brandsma JL, Hanlon DJ. Generation of dendritic cells from rabbit bone marrow mononuclear cell cultures supplemented with hGM-CSF and hIL-4. Veterinary Immunology And Immunopathology 2005, 103: 163-172. PMID: 15621303, DOI: 10.1016/j.vetimm.2004.08.022.Peer-Reviewed Original ResearchConceptsBone marrow mononuclear cellsDendritic cellsDC vaccinesAllogeneic T cell proliferationCottontail rabbit papillomavirus modelDownregulation of CD14Therapeutic vaccination strategiesUseful preclinical dataMononuclear cell culturesT cell proliferationMHC class IIRabbit bone marrow mononuclear cellsMarrow mononuclear cellsRelevant animal modelsCytokines GM-CSFDC vaccinationCervical cancerLymphocyte reactionHuman papillomavirusMononuclear cellsPreclinical dataIL-4Vaccination strategiesNon-specific esterase activityAnimal models