2023
Chemiexcited Neurotransmitters and Hormones Create DNA Photoproducts in the Dark
Gonçalves L, Angelé-Martínez C, Premi S, Palmatier M, Prado F, Di Mascio P, Bastos E, Brash D. Chemiexcited Neurotransmitters and Hormones Create DNA Photoproducts in the Dark. ACS Chemical Biology 2023, 18: 484-493. PMID: 36775999, PMCID: PMC10276651, DOI: 10.1021/acschembio.2c00787.Peer-Reviewed Original ResearchConceptsSinglet molecular oxygenOxidation of serotoninMolecular oxygenElectron excitationTriplet stateAdjacent pyrimidine basesAbsence of lightEnergy transferDark processPyrimidine basesSkin pigment melaninBiochemical reactionsMoleculesEnergy levelsCatecholamine neurotransmittersBiomoleculesCycloadditionUltravioletMammalian metabolismCyclobutane pyrimidine dimersOxidationAminesPigment melaninRadicalsPeroxynitrite
2019
Defective postreplication repair of UV photoproducts in melanoma: a mutator phenotype
Brash DE, Seidman MM. Defective postreplication repair of UV photoproducts in melanoma: a mutator phenotype. Molecular Oncology 2019, 14: 5-7. PMID: 31821728, PMCID: PMC6944110, DOI: 10.1002/1878-0261.12612.Commentaries, Editorials and Letters
2015
Chemiexcitation of melanin derivatives induces DNA photoproducts long after UV exposure
Premi S, Wallisch S, Mano CM, Weiner AB, Bacchiocchi A, Wakamatsu K, Bechara EJ, Halaban R, Douki T, Brash DE. Chemiexcitation of melanin derivatives induces DNA photoproducts long after UV exposure. Science 2015, 347: 842-847. PMID: 25700512, PMCID: PMC4432913, DOI: 10.1126/science.1256022.Peer-Reviewed Original ResearchConceptsDark cyclobutane pyrimidine dimersExcited electronic statesUltraviolet photonsUV photonsElectronic statesTriplet stateSunlight-induced melanomaCytosine-containing cyclobutane pyrimidine dimersEnergy transferPhotonsPicosecondsElectronsUV exposureRadiationChemiexcitationEnergyStatePhotoproducts
2014
Enhancing the detection of barcoded reads in high throughput DNA sequencing data by controlling the false discovery rate
Buschmann T, Zhang R, Brash DE, Bystrykh LV. Enhancing the detection of barcoded reads in high throughput DNA sequencing data by controlling the false discovery rate. BMC Bioinformatics 2014, 15: 264. PMID: 25099007, PMCID: PMC4133078, DOI: 10.1186/1471-2105-15-264.Peer-Reviewed Original Research
2010
Human Telomeres Are Hypersensitive to UV-Induced DNA Damage and Refractory to Repair
Rochette PJ, Brash DE. Human Telomeres Are Hypersensitive to UV-Induced DNA Damage and Refractory to Repair. PLOS Genetics 2010, 6: e1000926. PMID: 20442874, PMCID: PMC2861706, DOI: 10.1371/journal.pgen.1000926.Peer-Reviewed Original ResearchConceptsUV-induced DNA damageDNA damageTelomeric repeatsHuman telomeresExcision repairTelomeric repeat unitsNucleotide excision repairDouble-strand breaksUV-induced CPDGenome integrityGenomic integrityExcision repair sitesMitochondrial DNARegion of p53Opposite strandTelomeresCPD removalUnrepaired lesionsRepeatsPreeminent risk factorUV sensitivityPyrimidine dimersCancer developmentRepeat unitsCritical role
2004
Donor DNA in a renal cell carcinoma metastasis from a bone marrow transplant recipient
Chakraborty A, Lazova R, Davies S, Bäckvall H, Ponten F, Brash D, Pawelek J. Donor DNA in a renal cell carcinoma metastasis from a bone marrow transplant recipient. Bone Marrow Transplantation 2004, 34: 183-186. PMID: 15195072, DOI: 10.1038/sj.bmt.1704547.Peer-Reviewed Original Research
2001
The DNA Damage Signal for Mdm2 Regulation, Trp53 Induction, and Sunburn Cell Formation In Vivo Originates from Actively Transcribed Genes
Brash D, Wikonkal N, Remenyik E, van der Horst G, Friedberg E, Cheo D, van Steeg H, Westerman A, van Kranen H. The DNA Damage Signal for Mdm2 Regulation, Trp53 Induction, and Sunburn Cell Formation In Vivo Originates from Actively Transcribed Genes. Journal Of Investigative Dermatology 2001, 117: 1234-1240. PMID: 11710938, DOI: 10.1046/j.0022-202x.2001.01554.x.Peer-Reviewed Original ResearchConceptsDNA photoproductsDNA damage signalsUnrepaired DNA lesionsCell formationSpecific genome regionsTumor suppressor proteinCsb-/- miceUltraviolet-induced apoptosisNucleotide excision repair genesApoptosis signal pathwayExcision repair genesActive genesMutant cellsGenome regionsDNA repairSuppressor proteinDamage signalsMDM2 regulationWild typeDNA lesionsPrevents cellsHomozygous inactivationGenesRepair genesDNA signals
1999
Ultraviolet Radiation Induced Signature Mutations in Photocarcinogenesis
Wikonkal N, Brash D. Ultraviolet Radiation Induced Signature Mutations in Photocarcinogenesis. Journal Of Investigative Dermatology Symposium Proceedings 1999, 4: 6-10. PMID: 10537000, DOI: 10.1038/sj.jidsp.5640173.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsSignature mutationsSkin cancerNon-melanoma skin cancerUV-signature mutationsClinical dataSubstantial burdenSkin carcinogenesisMurine epidermisNormal individualsNormal humansCancerCancer developmentTumor suppressor geneClonal expansionTumor promoterTP53Suppressor geneGenetic eventsMutationsCells
1990
Rapid repair kinetics of pyrimidine(6–4)pyrimidone photoproducts in human cells are due to excision rather than conformational change
Mitchell D, Brash D, Nairn R. Rapid repair kinetics of pyrimidine(6–4)pyrimidone photoproducts in human cells are due to excision rather than conformational change. Nucleic Acids Research 1990, 18: 963-971. PMID: 2315046, PMCID: PMC330351, DOI: 10.1093/nar/18.4.963.Peer-Reviewed Original ResearchDefective DNA Repair in Humans: Clinical and Molecular Studies of Xeroderma Pigmentosum
Kraemer K, Seetharam S, Seidman M, Bredberg A, Brash D, Waters H, Protić-Sablijć M, Peck G, DiGiovanna J, Moshell A, Tarone R, Jones G, Parshad R, Sanford K. Defective DNA Repair in Humans: Clinical and Molecular Studies of Xeroderma Pigmentosum. Basic Life Sciences 1990, 53: 95-104. PMID: 2282051, DOI: 10.1007/978-1-4613-0637-5_7.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus Statements
1986
The C-C (6-4) UV photoproduct is mutagenic in Escherichia coli.
Glickman BW, Schaaper RM, Haseltine WA, Dunn RL, Brash DE. The C-C (6-4) UV photoproduct is mutagenic in Escherichia coli. Proceedings Of The National Academy Of Sciences Of The United States Of America 1986, 83: 6945-6949. PMID: 3529093, PMCID: PMC386627, DOI: 10.1073/pnas.83.18.6945.Peer-Reviewed Original Research
1985
Escherichia coli DNA photolyase reverses cyclobutane pyrimidine dimers but not pyrimidine-pyrimidone (6-4) photoproducts.
Brash DE, Franklin WA, Sancar GB, Sancar A, Haseltine WA. Escherichia coli DNA photolyase reverses cyclobutane pyrimidine dimers but not pyrimidine-pyrimidone (6-4) photoproducts. Journal Of Biological Chemistry 1985, 260: 11438-11441. PMID: 3900062, DOI: 10.1016/s0021-9258(17)39046-4.Peer-Reviewed Original Research
1984
Longevity-dependent organ-specific accumulation of DNA damage in two closely related murine species
Su C, Brash D, Turturro A, Hart R. Longevity-dependent organ-specific accumulation of DNA damage in two closely related murine species. Mechanisms Of Ageing And Development 1984, 27: 239-247. PMID: 6492898, DOI: 10.1016/0047-6374(84)90049-6.Peer-Reviewed Original Research
1983
Induction of single-strand breaks plus alkali-labile bonds by N-nitrosoureas in rat tissues in vivo: Ethylnitrosourea versus benzylnitrosourea
Su CM, Brash DE, Chang M, Hart RW, D'Ambrosio SM. Induction of single-strand breaks plus alkali-labile bonds by N-nitrosoureas in rat tissues in vivo: Ethylnitrosourea versus benzylnitrosourea. Mutation Research/Fundamental And Molecular Mechanisms Of Mutagenesis 1983, 108: 1-12. PMID: 6835219, DOI: 10.1016/0027-5107(83)90105-7.Peer-Reviewed Original Research
1982
Determination of DNA superhelicity and extremely low levels of DNA strand breaks in low numbers of nonradiolabeled cells by DNA-4′,6-diamidino-2-phenylindole fluorescence in nucleoid gradients
Lipetz P, Brash D, Joseph L, Jewett H, Lisle D, Lantry L, Hart R, Stephens R. Determination of DNA superhelicity and extremely low levels of DNA strand breaks in low numbers of nonradiolabeled cells by DNA-4′,6-diamidino-2-phenylindole fluorescence in nucleoid gradients. Analytical Biochemistry 1982, 121: 339-348. PMID: 7103066, DOI: 10.1016/0003-2697(82)90491-2.Peer-Reviewed Original ResearchNew Approaches to DNA Damage and Repair: The Ultraviolet Light Example
Haseltine W, Gordon L, Lindan C, Lippke J, Brash D, Lo K, Royer-Pokora B. New Approaches to DNA Damage and Repair: The Ultraviolet Light Example. 1982, 20: 315-332. PMID: 7115270, DOI: 10.1007/978-1-4613-3476-7_21.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus Statements
1981
Distribution of UV light-induced damage in a defined sequence of human DNA: detection of alkaline-sensitive lesions at pyrimidine nucleoside-cytidine sequences.
Lippke JA, Gordon LK, Brash DE, Haseltine WA. Distribution of UV light-induced damage in a defined sequence of human DNA: detection of alkaline-sensitive lesions at pyrimidine nucleoside-cytidine sequences. Proceedings Of The National Academy Of Sciences Of The United States Of America 1981, 78: 3388-3392. PMID: 6943547, PMCID: PMC319573, DOI: 10.1073/pnas.78.6.3388.Peer-Reviewed Original Research