2023
Chemiexcited Neurotransmitters and Hormones Create DNA Photoproducts in the Dark
Gonçalves L, Angelé-Martínez C, Premi S, Palmatier M, Prado F, Di Mascio P, Bastos E, Brash D. Chemiexcited Neurotransmitters and Hormones Create DNA Photoproducts in the Dark. ACS Chemical Biology 2023, 18: 484-493. PMID: 36775999, PMCID: PMC10276651, DOI: 10.1021/acschembio.2c00787.Peer-Reviewed Original ResearchConceptsSinglet molecular oxygenOxidation of serotoninMolecular oxygenElectron excitationTriplet stateAdjacent pyrimidine basesAbsence of lightEnergy transferDark processPyrimidine basesSkin pigment melaninBiochemical reactionsMoleculesEnergy levelsCatecholamine neurotransmittersBiomoleculesCycloadditionUltravioletMammalian metabolismCyclobutane pyrimidine dimersOxidationAminesPigment melaninRadicalsPeroxynitriteChemiexcitation: Mammalian Photochemistry in the Dark†
Brash D, Goncalves L. Chemiexcitation: Mammalian Photochemistry in the Dark†. Photochemistry And Photobiology 2023, 99: 251-276. PMID: 36681894, PMCID: PMC10065968, DOI: 10.1111/php.13781.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsExcited statesMammalian cellsCyclobutane pyrimidine dimersEvolutionary selectionBond rearrangementUnoccupied orbitalsGround stateReaction productsPyrimidine dimersChemiexcitationRecent findingsBiologyPathogenic eventsRadicalsUltraviolet lightMoleculesDrug-induced deafnessPotential pathogenesisCellsMelaninAchilles heelMammalsBiomoleculesPhotochemistryDNA
2022
Cyclobutane Pyrimidine Dimer Hyperhotspots as Sensitive Indicators of Keratinocyte UV Exposure†
Garcia‐Ruiz A, Kornacker K, Brash DE. Cyclobutane Pyrimidine Dimer Hyperhotspots as Sensitive Indicators of Keratinocyte UV Exposure†. Photochemistry And Photobiology 2022, 98: 987-997. PMID: 35944237, PMCID: PMC9802031, DOI: 10.1111/php.13683.Peer-Reviewed Original ResearchConceptsCyclobutane pyrimidine dimersGenomic averageSequence motifsHigh-throughput DNA sequencing methodsETS family transcription factorsNucleotide resolution analysisRNA processing genesNeonatal human epidermal keratinocytesDNA sequencing methodsTranscription factorsCpG islandsSites hundredsCell physiologyProcessing genesPromoter regionCell deathDNA damageSequencing methodsBiological importanceHuman epidermal keratinocytesPyrimidine dimersGenesMotifEpidermal keratinocytesMelanocytes
2019
Acetyl zingerone: An efficacious multifunctional ingredient for continued protection against ongoing DNA damage in melanocytes after sun exposure ends
Chaudhuri RK, Meyer T, Premi S, Brash D. Acetyl zingerone: An efficacious multifunctional ingredient for continued protection against ongoing DNA damage in melanocytes after sun exposure ends. International Journal Of Cosmetic Science 2019, 42: 36-45. PMID: 31538664, PMCID: PMC7004018, DOI: 10.1111/ics.12582.Peer-Reviewed Original ResearchConceptsSun exposureSolar-simulated ultraviolet radiationReactive oxygen speciesIntracellular levelsCyclobutane pyrimidine dimersΑ-tocopherolCPD formationTraditional sunscreensScavenge peroxynitriteUVR exposureOngoing DNA damageAntioxidant α-tocopherolUltraviolet radiationUVA radiationMelanocytesROS formationExposureQuench singlet oxygenUse of AZEfficacyOxygen speciesKeratinocytesDNA damageFree radicalsHoursGenomic sites hypersensitive to ultraviolet radiation
Premi S, Han L, Mehta S, Knight J, Zhao D, Palmatier MA, Kornacker K, Brash DE. Genomic sites hypersensitive to ultraviolet radiation. Proceedings Of The National Academy Of Sciences Of The United States Of America 2019, 116: 24196-24205. PMID: 31723047, PMCID: PMC6883822, DOI: 10.1073/pnas.1907860116.Peer-Reviewed Original ResearchMeSH Keywords5' Untranslated RegionsCells, CulturedDNA DamageFibroblastsGene Expression RegulationGenome, HumanHigh-Throughput Nucleotide SequencingHumansMelanocytesMelanomaMutationPromoter Regions, GeneticProtein BiosynthesisPyrimidine DimersPyrimidine NucleotidesSkin NeoplasmsTOR Serine-Threonine KinasesUltraviolet RaysConceptsCyclobutane pyrimidine dimersETS family transcription factorsIndividual gene promotersFamily transcription factorsRNA-binding proteinPrimary human melanocytesSingle-base resolutionEpigenetic marksGenomic averageTranslation regulationGenomic sitesMotif locationsTranscription factorsCell physiologyGene promoterCancer driversGenomeHuman melanocytesCell typesTumor evolutionCell pathwaysRare mutationsUV targetPyrimidine dimersApurinic sites
2018
Chemiexcitation and Its Implications for Disease
Brash DE, Goncalves LCP, Bechara EJH, Group T. Chemiexcitation and Its Implications for Disease. Trends In Molecular Medicine 2018, 24: 527-541. PMID: 29751974, PMCID: PMC5975183, DOI: 10.1016/j.molmed.2018.04.004.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsHigh-energy processesQuantum mechanicsMolecular orbitalsTransfer energyHighest energy molecular orbitalChemiexcitationUltraviolet lightMutagenic cyclobutane pyrimidine dimersNumerous human diseasesPhotonsElectronsCyclobutane pyrimidine dimersOrbitalsHuman diseasesUpstream eventsPigment melaninEnergy
1995
Intragenic Domains of Strand-specific Repair inEscherichia coli
Kunala S, Brash D. Intragenic Domains of Strand-specific Repair inEscherichia coli. Journal Of Molecular Biology 1995, 246: 264-272. PMID: 7869378, DOI: 10.1006/jmbi.1994.0082.Peer-Reviewed Original ResearchConceptsTranscription-repair coupling factorTranscription start siteStart siteEscherichia coli genesUV-induced cyclobutane pyrimidine dimersStrand-specific repairMfd mutationExcision repair mechanismChromatin domainsIntragenic domainsGenomic organizationMfd geneColi geneCyclobutane pyrimidine dimersLacZ transcriptionDNA repairInEscherichia coliDownstream domainIndividual nucleotidesRepair mechanismsGenesTranscriptionRepair systemDNA strandsInduced levels
1988
UV MUTAGENIC PHOTOPRODUCTS IN Escherichia coli AND HUMAN CELLS: A MOLECULAR GENETICS PERSPECTIVE ON HUMAN SKIN CANCER*
Brash D. UV MUTAGENIC PHOTOPRODUCTS IN Escherichia coli AND HUMAN CELLS: A MOLECULAR GENETICS PERSPECTIVE ON HUMAN SKIN CANCER*. Photochemistry And Photobiology 1988, 48: 59-66. PMID: 3064116, DOI: 10.1111/j.1751-1097.1988.tb02786.x.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus Statements
1985
Escherichia coli DNA photolyase reverses cyclobutane pyrimidine dimers but not pyrimidine-pyrimidone (6-4) photoproducts.
Brash DE, Franklin WA, Sancar GB, Sancar A, Haseltine WA. Escherichia coli DNA photolyase reverses cyclobutane pyrimidine dimers but not pyrimidine-pyrimidone (6-4) photoproducts. Journal Of Biological Chemistry 1985, 260: 11438-11441. PMID: 3900062, DOI: 10.1016/s0021-9258(17)39046-4.Peer-Reviewed Original ResearchConceptsPyrimidine (6-4) pyrimidone photoproductsE. coli DNA photolyaseDNA photolyaseEscherichia coli DNA photolyasePhr geneCyclobutane pyrimidine dimersEnd-labeled DNAMolecular lesionsPhotoreactivation of Escherichia coli reverses umuC induction by UV light
Brash DE, Haseltine WA. Photoreactivation of Escherichia coli reverses umuC induction by UV light. Journal Of Bacteriology 1985, 163: 460-463. PMID: 2991189, PMCID: PMC219144, DOI: 10.1128/jb.163.2.460-463.1985.Peer-Reviewed Original ResearchConceptsCyclobutane pyrimidine dimersUmuC inductionPyrimidine dimersUV mutagenesisE. coli lacZ geneOperon fusionsGene transcriptionGene productsSOS responseLesionsLacZ gene