2024
Utility of Cerebrospinal Fluid Unstimulated Interferon-Gamma (IRISA-TB) as a Same-Day Test for Tuberculous Meningitis in a Tuberculosis-Endemic, Resource-Poor Setting
Randall P, Mutsvangwa J, Nliwasa M, Wilson L, Makamure B, Makambwa E, Meldau R, Dheda K, Munyati S, Siddiqi O, Corbett E, Esmail A. Utility of Cerebrospinal Fluid Unstimulated Interferon-Gamma (IRISA-TB) as a Same-Day Test for Tuberculous Meningitis in a Tuberculosis-Endemic, Resource-Poor Setting. Open Forum Infectious Diseases 2024, 11: ofae496. PMID: 39286031, PMCID: PMC11403475, DOI: 10.1093/ofid/ofae496.Peer-Reviewed Original ResearchUnstimulated interferon-gammaTuberculous meningitisPolymerase chain reaction productsCerebrospinal fluidXpert UltraDNA-based assaysInterferon-gammaHuman immunodeficiency virus (HIV)-infectedSuspected TBM patientsRule-in testPositive predictive valueGeneXpert UltraStage 2 evaluationTBM patientsSame-day testingNon-TBMZambian samplesUninfected participantsDiagnostic performanceAfrican samplesImmunodiagnostic assaysXpertMicrobiological resultsAssayBiobank samples
2021
Multi-frequency impedance sensing for detection and sizing of DNA fragments
Sui J, Gandotra N, Xie P, Lin Z, Scharfe C, Javanmard M. Multi-frequency impedance sensing for detection and sizing of DNA fragments. Scientific Reports 2021, 11: 6490. PMID: 33753781, PMCID: PMC7985362, DOI: 10.1038/s41598-021-85755-9.Peer-Reviewed Original ResearchConceptsTarget molecule bindsComplex biological samplesElectronic biosensorsNon-target materialsProbe selectivityAnalytical performanceMolecules bindBiological samplesReaction productsDifferent lengthsDNA moleculesDNA detectionMicrofluidic chipNonspecific bindingFmolElectronic signalsParamagnetic beadsImpedance sensorBeadsBiosensorDNA concentrationDNA fragmentsSelectivityProbePolymerase chain reaction products
2014
The molecular genetic analysis of the expanding pachyonychia congenita case collection
Wilson N, O'Toole E, Milstone L, Hansen C, Shepherd A, Al‐Asadi E, Schwartz M, McLean W, Sprecher E, Smith F. The molecular genetic analysis of the expanding pachyonychia congenita case collection. British Journal Of Dermatology 2014, 171: 343-355. PMID: 24611874, PMCID: PMC4282083, DOI: 10.1111/bjd.12958.Peer-Reviewed Original ResearchConceptsPachyonychia congenitaInternational Pachyonychia Congenita Research RegistryClinical diagnosisPeripheral blood leucocytesFollicular keratosisResearch RegistryBlood leucocytesOral leucokeratosisNail dystrophyKeratin mutationsUnreported mutationsPalmoplantar keratodermaChain reaction productsCase collectionDiagnosisKRT6CKRT6APolymerase chain reaction productsMolecular genetic analysisKRT6BKRT16KRT17Keratin genesMutationsTotal number
2005
Mitochondrial DNA depletion analysis by pseudogene ratioing
Swerdlow R, Redpath G, Binder D, Davis J, VandenBerg S. Mitochondrial DNA depletion analysis by pseudogene ratioing. Journal Of Neuroscience Methods 2005, 150: 265-271. PMID: 16118020, DOI: 10.1016/j.jneumeth.2005.06.023.Peer-Reviewed Original ResearchConceptsPlasmid clonesCell linesTreated with ethidium bromideCopies of mtDNAPolymerase chain reactionMtDNA copy numberPCR-based analysisPolymerase chain reaction productsU251 human glioma cell lineMtDNA pseudogenesAmplified mtDNAMitochondrial DNAMtDNAMtDNA depletionGenomic DNACopy numberHuman glioma cell linesPseudogenesGlioma cell linesEthidium bromidePrimersPlasmidClonesDNAChain reactionAmplification of human DNA by primers targeted to Leishmania kinetoplast DNA and post-genome considerations in the detection of parasites by a polymerase chain reaction.
Vergel C, Walker J, SARAVIA NG. Amplification of human DNA by primers targeted to Leishmania kinetoplast DNA and post-genome considerations in the detection of parasites by a polymerase chain reaction. American Journal Of Tropical Medicine And Hygiene 2005, 72: 423-9. PMID: 15827280, DOI: 10.4269/ajtmh.2005.72.423.Peer-Reviewed Original ResearchConceptsMolecular amplification techniquesPerformance of primersPolymerase chain reaction productsAmplification techniquesMonocyte DNADNA probesLeishmania kinetoplast DNANew applicationsChain reaction productsDNA amplificationHuman DNA sequencesDNA sequencesDetection of parasitesPolymerase chain reaction
1998
Analyses of Cross Species Polymerase Chain Reaction Products to Infer the Ancestral State of Human Polymorphisms
Iyengar S, Seaman M, Deinard A, Rosenbaum H, Sirugo G, Castiglione C, Kidd J, Kidd K. Analyses of Cross Species Polymerase Chain Reaction Products to Infer the Ancestral State of Human Polymorphisms. Mitochondrial DNA Part A 1998, 8: 317-327. PMID: 10993602, DOI: 10.3109/10425179809034076.Peer-Reviewed Original ResearchConceptsSingle-strand conformational polymorphismSequence dataUntranslated regionHuman polymorphismsSingle ancestral allelePolymorphic allelesRestriction enzyme sitesAncestral stateExtant populationsGenomic regionsAncestral statusAncestral onesAncestral alleleHuman sequenceHuman allelesStrand conformational polymorphismPCR-RFLPsRestriction fragment length analysisPolymorphic sitesNumerous populationsEnzyme sitesAllelesSpeciesConformational polymorphismPolymerase chain reaction products
1995
Acid incubation increases NHE-3 mRNA abundance in OKP cells
Amemiya M, Yamaji Y, Cano A, Moe OW, Alpern RJ. Acid incubation increases NHE-3 mRNA abundance in OKP cells. American Journal Of Physiology 1995, 269: c126-c133. PMID: 7631739, DOI: 10.1152/ajpcell.1995.269.1.c126.Peer-Reviewed Original Research
1990
Activation of c-Ki-ras in human gastrointestinal dysplasias determined by direct sequencing of polymerase chain reaction products.
Meltzer S, Mane S, Wood P, Resau J, Newkirk C, Terzakis J, Korelitz B, Weinstein W, Needleman S. Activation of c-Ki-ras in human gastrointestinal dysplasias determined by direct sequencing of polymerase chain reaction products. Cancer Research 1990, 50: 3627-30. PMID: 2187599.Peer-Reviewed Original ResearchConceptsC-Ki-rasUlcerative colitisBarrett's esophagusChain reaction productsPolymerase chain reaction productsSporadic colon cancerGastrointestinal lesionsPolymerase chain reactionEsophageal adenocarcinomaColonic specimensCodon 13Direct dideoxy sequencingColon cancerUncommon eventMalignant progressionCodon 12C-Ha-rasEsophagusPoint mutationsDysplasiaDisease statesChain reactionColitisDirect sequencingCancer
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