2021
Monitoring phagocytic uptake of amyloid β into glial cell lysosomes in real time
Prakash P, Jethava K, Korte N, Izquierdo P, Favuzzi E, Rose I, Guttenplan K, Manchanda P, Dutta S, Rochet J, Fishell G, Liddelow S, Attwell D, Chopra G. Monitoring phagocytic uptake of amyloid β into glial cell lysosomes in real time. Chemical Science 2021, 12: 10901-10918. PMID: 34476070, PMCID: PMC8372545, DOI: 10.1039/d1sc03486c.Peer-Reviewed Original ResearchAmyloid-bAlzheimer's diseaseStages of ADOrganelles of cellsRegulating brain functionPhagocytic mechanismsRemoved ABGlial cellsAcidic organellesCell lysosomesGreen fluorescenceExtracellular spaceImpaired phagocytic functionWorsened disease outcomeLive animalsPhagocytosisCellsPhagocytic functionNon-fluorescentLysosomesEnzymePhysiological pH.AlzheimerDisease outcomeMechanism
2013
High-speed laser microsurgery of alert fruit flies for fluorescence imaging of neural activity
Sinha S, Liang L, Ho ET, Urbanek KE, Luo L, Baer TM, Schnitzer MJ. High-speed laser microsurgery of alert fruit flies for fluorescence imaging of neural activity. Proceedings Of The National Academy Of Sciences Of The United States Of America 2013, 110: 18374-18379. PMID: 24167298, PMCID: PMC3832030, DOI: 10.1073/pnas.1216287110.Peer-Reviewed Original ResearchConceptsLive animalsCellular functionsFruit flySmall organismsMushroom bodiesKenyon cellsFliesAntennal lobeLive organismsOrganismsNeuronal plasticityHigh-throughput preparationMouse craniumExperimental throughputFluorescence imagingMicrosurgical openingLaser microsurgeryAntsVivo microscopy studiesNematodesAnimalsTwo-photon microscopySpeciesPhototaxisOptical experimentation
2012
High-throughput fluorescence-based isolation of live C. elegans larvae
Fernandez A, Bargmann B, Mis E, Edgley M, Birnbaum K, Piano F. High-throughput fluorescence-based isolation of live C. elegans larvae. Nature Protocols 2012, 7: 1502-1510. PMID: 22814389, PMCID: PMC5274720, DOI: 10.1038/nprot.2012.084.Peer-Reviewed Original ResearchConceptsGenetic interactionsChemical genetic screenNematode Caenorhabditis elegansFluorescence-activated cell sortingSelection of animalsTermination mutantsCaenorhabditis elegansSorting animalsGenetic screeningChemical screeningGFP expressionCell sortingLarval stageLive animalsHigh-speed sortingMutantsAnimalsFACSIsolatesGenotypesNematodesHomozygotes
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