2012
Purification of DNA-origami nanostructures by rate-zonal centrifugation
Lin C, Perrault SD, Kwak M, Graf F, Shih WM. Purification of DNA-origami nanostructures by rate-zonal centrifugation. Nucleic Acids Research 2012, 41: e40-e40. PMID: 23155067, PMCID: PMC3553994, DOI: 10.1093/nar/gks1070.Peer-Reviewed Original ResearchConceptsDNA origami nanostructuresDNA nanostructuresDNA origami structuresAgarose gel electrophoresisDNA origami constructsNanostructuresAqueous solutionFraction collectionSeparation resolutionGradient mixingPurification processHigh yieldsPurification approachConcentration stepRate zonal centrifugationCentrifuge tubeAgarose gelDyeAvailable equipmentDesaltingGelContaminantsPurificationPreparationSeparation
2007
Novel Approach to Performing Metabolite Identification in Drug Metabolism
Nassar A, Lee D. Novel Approach to Performing Metabolite Identification in Drug Metabolism. Journal Of Chromatographic Science 2007, 45: 113-119. PMID: 17462124, DOI: 10.1093/chromsci/45.3.113.Peer-Reviewed Original ResearchConceptsMetabolite identificationHuman liver microsomal incubationsMetabolite identification studiesDrug discoveryMS fragmentation patternsLiver microsomal incubationsLimit of detectionSite of metabolismDynamic-flow methodMass spectrometric analysisMultistage fragmentationMass spectrometerFragmentation patternsMicrosomal incubationsOnline separationChromatographic peaksMass spectrometryMinor metabolitesSpectrometric analysisFraction collectionO-dealkylationRadioactivity detectorMetabolite of dextromethorphanRadioactivity detectionColumn pressure
2002
Evaluation of Multidimensional Chromatography Coupled with Tandem Mass Spectrometry (LC/LC−MS/MS) for Large-Scale Protein Analysis: The Yeast Proteome
Peng J, Elias J, Thoreen C, Licklider L, Gygi S. Evaluation of Multidimensional Chromatography Coupled with Tandem Mass Spectrometry (LC/LC−MS/MS) for Large-Scale Protein Analysis: The Yeast Proteome. Journal Of Proteome Research 2002, 2: 43-50. PMID: 12643542, DOI: 10.1021/pr025556v.Peer-Reviewed Original ResearchConceptsStrong cation exchangeTandem mass spectrometryMass spectrometryMS/MS spectraComplex protein mixturesReversed-phase chromatographyTwo-dimensional separationMS/MS analysisMultidimensional chromatographyMS/MSMS spectraFraction collectionCation exchangeProtein mixturesSCX chromatographyRP-LCLiquid chromatographyMS analysisChromatographyPeptide identificationSEQUEST algorithmSpectrometryYeast protein databaseSingle analysisLarge-scale protein analysis
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