2019
Characterization of buffalo native pregnancy-associated glycoprotein: mass spectrometry-based glycan composition analysis, sugar-binding characteristics and proteolytic activity assay
Lotfan M, Choudhary S, Yadav M, Tripathi I, Bhan S, Bathla S, Rawat P, Kumar S, Mohanty T, Kaushik J, Mohanty A. Characterization of buffalo native pregnancy-associated glycoprotein: mass spectrometry-based glycan composition analysis, sugar-binding characteristics and proteolytic activity assay. Journal Of Proteins And Proteomics 2019, 10: 23-32. DOI: 10.1007/s42485-018-00003-5.Peer-Reviewed Original ResearchPregnancy-associated glycoproteinsSugar-binding characteristicsProteolytic activityHigh-resolution mass spectrometryN-glycan structuresO-glycan structuresProteolytic activity assaysGlycosylated secretory proteinsPresence of glycosylationInhibitor pepstatin AGlycan structuresSecretory proteinsGlycan analysisActive peptidaseEmission maximumAffinity chromatographyMass spectrometryFluorescence quenching assayFoetal cotyledonsPepstatin ASDS-PAGEAnion exchangeOuter epithelial cell layerActivity assayPH range
2008
In Vitro Selection and Characterization of Cellulose-Binding RNA Aptamers Using isothermal Amplification
Boese B, Corbino K, Breaker R. In Vitro Selection and Characterization of Cellulose-Binding RNA Aptamers Using isothermal Amplification. Nucleosides Nucleotides & Nucleic Acids 2008, 27: 949-966. PMID: 18696364, PMCID: PMC5360192, DOI: 10.1080/15257770802257903.Peer-Reviewed Original ResearchConceptsRNA aptamersRibozyme cleavage productsCellulose affinity chromatographyIsolation of RNARapid amplificationVitro SelectionRibozyme functionRibozyme sequenceFunctional nucleic acidsNucleic acid amplification protocolsRNARobust bindingAffinity chromatographyCleavage productsNucleic acidsSequence replicationBindingSignificant bindingAptamerAmplification protocolAmplification
2000
Matricellular Proteins as Modulators of Cell–Matrix Interactions: Adhesive Defect in Thrombospondin 2-null Fibroblasts is a Consequence of Increased Levels of Matrix Metalloproteinase-2
Yang Z, Kyriakides T, Bornstein P. Matricellular Proteins as Modulators of Cell–Matrix Interactions: Adhesive Defect in Thrombospondin 2-null Fibroblasts is a Consequence of Increased Levels of Matrix Metalloproteinase-2. Molecular Biology Of The Cell 2000, 11: 3353-3364. PMID: 11029041, PMCID: PMC14997, DOI: 10.1091/mbc.11.10.3353.Peer-Reviewed Original ResearchConceptsCell-matrix interactionsAbnormal cell-matrix interactionsWild-type cellsTSP2-null miceAdhesive defectsComplex phenotypesCell spreadingMolecular mechanismsMutant fibroblastsProtein resultsMatrix proteinsStable transfectionMatrix metalloproteinasesMetalloproteinase-2Matricellular proteinAltered expressionTHBS2 geneNull miceAffinity chromatographyGelatinolytic assaysThrombospondin-2TSP2Matrix metalloproteinase-2FibroblastsLevels of MMP2
1997
Calmodulin Activates Phosphatidylinositol 3-Kinase*
Joyal J, Burks D, Pons S, Matter W, Vlahos C, White M, Sacks D. Calmodulin Activates Phosphatidylinositol 3-Kinase*. Journal Of Biological Chemistry 1997, 272: 28183-28186. PMID: 9353264, DOI: 10.1074/jbc.272.45.28183.Peer-Reviewed Original ResearchConceptsSrc homology 2 domainIntact cellsPhosphorylation of phosphatidylinositolActivates PhosphatidylinositolVesicular traffickingEukaryotic cellsEffector proteinsRegulatory subunitCytoskeletal organizationUbiquitous Ca2PhosphatidylinositolIntracellular eventsNovel mechanismAffinity chromatographyGrowth factorCalmodulinCalmodulin antagonistsMultiple processesCellsCoimmunoprecipitationDirect linkPhosphorylationTraffickingSubunitsCa2Synapsin I interacts with c-Src and stimulates its tyrosine kinase activity
Onofri F, Giovedì S, Vaccaro P, Czernik A, Valtorta F, De Camilli P, Greengard P, Benfenati F. Synapsin I interacts with c-Src and stimulates its tyrosine kinase activity. Proceedings Of The National Academy Of Sciences Of The United States Of America 1997, 94: 12168-12173. PMID: 9342381, PMCID: PMC23739, DOI: 10.1073/pnas.94.22.12168.Peer-Reviewed Original ResearchConceptsTyrosine kinase activityC-SrcKinase activitySynapsin ISrc homology 3 domainNonreceptor tyrosine kinase c-SrcSynaptic vesicle-associated phosphoproteinsTyrosine kinase c-SrcSynaptic vesiclesC-Src SH3 domainKinase c-SrcEndogenous tyrosine kinase activityTotal cellular poolStoichiometric phosphorylationSynaptic vesicle preparationsCoprecipitation assaysSignal transductionProtein speciesCellular poolRegulatory sitesBinding proteinVesicle preparationsDetergent extractsSeveralfold stimulationAffinity chromatography
1996
Characterization of the Reductase Domain of Rat Neuronal Nitric Oxide Synthase Generated in the Methylotrophic Yeast Pichia pastoris CALMODULIN RESPONSE IS COMPLETE WITHIN THE REDUCTASE DOMAIN ITSELF*
Gachhui R, Presta A, Bentley D, Abu-Soud H, McArthur R, Brudvig G, Ghosh D, Stuehr D. Characterization of the Reductase Domain of Rat Neuronal Nitric Oxide Synthase Generated in the Methylotrophic Yeast Pichia pastoris CALMODULIN RESPONSE IS COMPLETE WITHIN THE REDUCTASE DOMAIN ITSELF*. Journal Of Biological Chemistry 1996, 271: 20594-20602. PMID: 8702805, DOI: 10.1074/jbc.271.34.20594.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceCalmodulinCalmodulin-Binding ProteinsDNA PrimersElectron Spin Resonance SpectroscopyFlavinsFlavoproteinsIsoenzymesMolecular Sequence DataNADH DehydrogenaseNeuronsNitric Oxide SynthaseOxidation-ReductionPichiaRatsRecombinant ProteinsSpectrometry, FluorescenceTryptophanConceptsElectron transferFlavin semiquinoneReductase domainNADPH-dependent flavin reductionArtificial electron acceptorsADP affinity chromatographyHeme-containing oxygenase domainCalmodulin responseNNOS reductase domainAnaerobic titrationFlavin reductionElectron acceptorNNOS reductaseFlavin-containing reductase domainReductase proteinSemiquinoneFlavinFlavin fluorescenceOxygenase domainAffinity chromatographyCytochrome c.Pure proteinCytochrome cTransferAcceptor
1994
Inactivation of a Cdk2 Inhibitor during Interleukin 2-Induced Proliferation of Human T Lymphocytes
Firpo E, Koff A, Solomon M, Roberts J. Inactivation of a Cdk2 Inhibitor during Interleukin 2-Induced Proliferation of Human T Lymphocytes. Molecular And Cellular Biology 1994, 14: 4889-4901. DOI: 10.1128/mcb.14.7.4889-4901.1994.Peer-Reviewed Original ResearchCyclin-dependent kinasesMitogenic signalingPromoter activityActivation of cell cycle proteinsCyclin-Cdk inhibitorCell cycle commitmentCyclin-CDK complexesCyclin-cdk2 complexesCell cycle proteinsG1 cyclin-CDK complexesCyclin-dependent kinase inhibitorNegative growth signalsT cell antigen receptorRegulating cell proliferationCell proliferationCDK2 inhibitorsAntigen receptor stimulationInhibitor of CDK2CDK2 activityCell cycleCycle proteinsRestriction pointGrowth signalsAffinity chromatographyMitogenic growth factorsp27Kip1, a cyclin-Cdk inhibitor, links transforming growth factor-beta and contact inhibition to cell cycle arrest.
Polyak K, Kato J, Solomon M, Sherr C, Massague J, Roberts J, Koff A. p27Kip1, a cyclin-Cdk inhibitor, links transforming growth factor-beta and contact inhibition to cell cycle arrest. Genes & Development 1994, 8: 9-22. PMID: 8288131, DOI: 10.1101/gad.8.1.9.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCDC2-CDC28 KinasesCell CommunicationCell CycleCell LineCyclin-Dependent Kinase 2Cyclin-Dependent Kinase-Activating KinaseCyclin-Dependent KinasesCyclinsMinkProtein Kinase InhibitorsProtein KinasesProtein Serine-Threonine KinasesProteinsProtein-Tyrosine KinasesSignal TransductionTransforming Growth Factor betaConceptsCdk2 complexesCyclin ECyclin D2-Cdk4 complexesCyclin-Cdk inhibitorCell-cell contactGrowth inhibitory signalsCell cycle arrestCatalytic subunitG1 progressionG1 cyclinsCell cycleCdk2 activationCycle arrestCDK2 inhibitionContact inhibitionCyclin E levelsActive complexAffinity chromatographyEpithelial cellsCDK2Inhibitory signalsCellsP27ComplexesActivation
1989
Purification and characterization of human lysosomal membrane glycoproteins
Mane S, Marzella L, Bainton D, Holt V, Cha Y, Hildreth J, August J. Purification and characterization of human lysosomal membrane glycoproteins. Archives Of Biochemistry And Biophysics 1989, 268: 360-378. PMID: 2912382, DOI: 10.1016/0003-9861(89)90597-3.Peer-Reviewed Original ResearchConceptsLysosomal membrane glycoproteinsCell surface expressionHL-60 cellsPlasma membraneU937 myelomonocytic leukemia cellsMembrane glycoproteinsHuman lysosomal membrane glycoproteinsU937 cellsAddition of AsnDetergent-extractable proteinTissue culture cellsNH2-terminal sequence analysisHLAMP-2Mouse cellsMembrane flowSequence analysisMyelomonocytic leukemia cellsAntibody affinity chromatographyCell typesCulture cellsPeroxidase-positive granulesProteinHLAMP-1Lysosomal structuresAffinity chromatography
1987
Purification and properties of a multifunctional calcium/calmodulin-dependent protein kinase from rat pancreas
Cohn J, Kinder B, Jamieson J, Delahunt N, Gorelick F. Purification and properties of a multifunctional calcium/calmodulin-dependent protein kinase from rat pancreas. Biochimica Et Biophysica Acta 1987, 928: 320-331. PMID: 3105599, DOI: 10.1016/0167-4889(87)90192-3.Peer-Reviewed Original ResearchConceptsCalmodulin protein kinaseCalcium/calmodulin-dependent protein kinaseCalmodulin-dependent protein kinaseProtein kinaseMultifunctional calcium/calmodulin-dependent protein kinaseTwo-dimensional gel electrophoresisRibosomal proteinsThreonine residuesLarge subunitCellular functionsRibosomal substratesSubstrate specificityKinaseProteolytic degradationSubunitsGel electrophoresisHydrophobic chromatographyAffinity chromatographyPolyacrylamide gelsCalmodulinPurification procedureGel filtrationEnzyme preparationAutophosphorylationTissue
1977
Human B cell alloantigens: separation from other membrane molecules by affinity chromatography
Cresswell P. Human B cell alloantigens: separation from other membrane molecules by affinity chromatography. European Journal Of Immunology 1977, 7: 636-639. PMID: 303569, DOI: 10.1002/eji.1830070911.Peer-Reviewed Original ResearchConceptsMolecular weight 35 000Sodium dodecyl sulfate gel electrophoresisDodecyl sulfate gel electrophoresisAffinity chromatographySulfate gel electrophoresisSoluble productsB-lymphoblastoid cell linesChromatographyMoleculesSodium deoxycholateLymphoblastoid cell linesGel electrophoresisAgarose columnIa-like alloantigensDeoxycholateCell linesHuman B cellsSeparationSpecific rabbit antibodiesB cellsColumnSerological activityElectrophoresisRabbit antibodiesMaterials
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