Characterization of buffalo native pregnancy-associated glycoprotein: mass spectrometry-based glycan composition analysis, sugar-binding characteristics and proteolytic activity assay
Lotfan M, Choudhary S, Yadav M, Tripathi I, Bhan S, Bathla S, Rawat P, Kumar S, Mohanty T, Kaushik J, Mohanty A. Characterization of buffalo native pregnancy-associated glycoprotein: mass spectrometry-based glycan composition analysis, sugar-binding characteristics and proteolytic activity assay. Journal Of Proteins And Proteomics 2019, 10: 23-32. DOI: 10.1007/s42485-018-00003-5.Peer-Reviewed Original ResearchPregnancy-associated glycoproteinsSugar-binding characteristicsProteolytic activityHigh-resolution mass spectrometryN-glycan structuresO-glycan structuresProteolytic activity assaysGlycosylated secretory proteinsPresence of glycosylationInhibitor pepstatin AGlycan structuresSecretory proteinsGlycan analysisActive peptidaseEmission maximumAffinity chromatographyMass spectrometryFluorescence quenching assayFoetal cotyledonsPepstatin ASDS-PAGEAnion exchangeOuter epithelial cell layerActivity assayPH range
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