2018
CLOCK phosphorylation by AKT regulates its nuclear accumulation and circadian gene expression in peripheral tissues
Luciano AK, Zhou W, Santana JM, Kyriakides C, Velazquez H, Sessa WC. CLOCK phosphorylation by AKT regulates its nuclear accumulation and circadian gene expression in peripheral tissues. Journal Of Biological Chemistry 2018, 293: 9126-9136. PMID: 29588368, PMCID: PMC5995495, DOI: 10.1074/jbc.ra117.000773.Peer-Reviewed Original ResearchConceptsCircadian locomotor output cycles kaputCLOCK phosphorylationCircadian gene expressionPhosphorylation sitesGene expressionNovel phosphorylation sitesClock-controlled genesCRISPR/Cas9 technologyNovel Akt substrateE-box elementsCore circadian genesAkt substrateTranscription factorsE-boxCentral circadian rhythmNuclear localizationNegative regulatorCas9 technologyNuclear accumulationCircadian genesInsulin-sensitive tissuesNuclear translocationPhosphorylationExpression levelsSer-845
2017
Application of CRISPR/Cas9 to the study of brain development and neuropsychiatric disease
Powell S, Gregory J, Akbarian S, Brennand K. Application of CRISPR/Cas9 to the study of brain development and neuropsychiatric disease. Molecular And Cellular Neuroscience 2017, 82: 157-166. PMID: 28549865, PMCID: PMC5516945, DOI: 10.1016/j.mcn.2017.05.007.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsCRISPR/Cas9 technologyPluripotent stem cellsTranscriptional regulatorsManipulation of DNAEpigenetic pathwaysGenomic editingSpecific lociCRISPR/Basic biologyCas9 technologyGene expressionStem cellsTargeted localizationEnzyme activityBrain developmentEpigenomeNeuropsychiatric diseasesGenomeCRISPRRepressionLociBiologyRegulatorEffectorsDNA
2016
Rab8 directs furrow ingression and membrane addition during epithelial formation in Drosophila melanogaster
Mavor L, Miao H, Zuo Z, Holly R, Xie Y, Loerke D, Blankenship J. Rab8 directs furrow ingression and membrane addition during epithelial formation in Drosophila melanogaster. Development 2016, 143: 892-903. PMID: 26839362, PMCID: PMC4813336, DOI: 10.1242/dev.128876.Peer-Reviewed Original ResearchMeSH KeywordsActinsAnimalsAnimals, Genetically ModifiedCell MembraneCRISPR-Cas SystemsCrosses, GeneticCytoplasmDrosophila melanogasterDrosophila ProteinsEmbryo, NonmammalianEpitheliumExocytosisFemaleGene Expression Regulation, DevelopmentalGolgi ApparatusGTP PhosphohydrolasesGuanosine TriphosphateMaleMembrane ProteinsMicroscopy, ConfocalProtein Structure, Tertiaryrab GTP-Binding ProteinsConceptsFurrow ingressionMembrane additionPlasma membranePlasma membrane furrowsLarge cytoplasmic aggregatesCRISPR/Cas9 technologyIntracellular trafficking pathwaysMembrane furrowsRab8 functionDrosophila embryosDrosophila melanogasterTrafficking pathwaysMembrane compartmentsEndogenous localizationProtein Rab11Early embryosCytoplasmic aggregatesCas9 technologyRab8Membrane storesCell surfaceEpithelial sheetsRab11Cell morphologyCompartmental behavior
This site is protected by hCaptcha and its Privacy Policy and Terms of Service apply