2025
Precision multiplexed base editing in human cells using Cas12a-derived base editors
Schweitzer A, Adams E, Nguyen M, Lek M, Isaacs F. Precision multiplexed base editing in human cells using Cas12a-derived base editors. Nature Communications 2025, 16: 5061. PMID: 40449999, PMCID: PMC12126522, DOI: 10.1038/s41467-025-59653-x.Peer-Reviewed Original ResearchConceptsMultiplex base editingBase editingBase editorsBase editor variantsMammalian genome engineeringBase editing technologyGenome engineering technologiesDNA double strand breaksBase editing systemMammalian genomesDouble strand breaksMultiple lociPolygenic phenotypesHuman cell linesHuman genomeGenome engineeringTarget nucleotideEditing outcomesMutation rateMultiplex editingMultiple gRNAsHuman cellsExpression cassetteEditing technologyGenome
2024
Expanding RNA editing toolkit using an IDR-based strategy
Di M, Lv J, Jing Z, Yang Y, Yan K, Wu J, Ge J, Rauch S, Dickinson B, Chi T. Expanding RNA editing toolkit using an IDR-based strategy. Molecular Therapy - Nucleic Acids 2024, 35: 102190. PMID: 38721279, PMCID: PMC11077028, DOI: 10.1016/j.omtn.2024.102190.Peer-Reviewed Original ResearchPositive selection CRISPR screens reveal a druggable pocket in an oligosaccharyltransferase required for inflammatory signaling to NF-κB
Lampson B, Ramίrez A, Baro M, He L, Hegde M, Koduri V, Pfaff J, Hanna R, Kowal J, Shirole N, He Y, Doench J, Contessa J, Locher K, Kaelin W. Positive selection CRISPR screens reveal a druggable pocket in an oligosaccharyltransferase required for inflammatory signaling to NF-κB. Cell 2024, 187: 2209-2223.e16. PMID: 38670073, PMCID: PMC11149550, DOI: 10.1016/j.cell.2024.03.022.Peer-Reviewed Original ResearchConceptsWhole-genome CRISPR-Cas9 screenCRISPR-Cas9 screensCryoelectron microscopy studiesCell surface localizationLipopolysaccharide receptor Toll-like receptor 4OST complexToll-like receptor 4CRISPR screensNF-kBCatalytic subunitN-glycosylationActivate NF-kBBase editorsUncompetitive inhibition mechanismNGI-1Molecular mechanismsCatalytic siteLPS-treated cellsOligosaccharyltransferaseDruggable pocketSTT3AReceptor Toll-like receptor 4Drug mechanism of actionStructural studiesInflammatory signaling
2019
APOBEC3A Loop 1 Is a Determinant for Single-Stranded DNA Binding and Deamination
Ziegler SJ, Hu Y, Devarkar SC, Xiong Y. APOBEC3A Loop 1 Is a Determinant for Single-Stranded DNA Binding and Deamination. Biochemistry 2019, 58: 3838-3847. PMID: 31448897, PMCID: PMC7211764, DOI: 10.1021/acs.biochem.9b00394.Peer-Reviewed Original ResearchConceptsSubstrate specificityLoop 1A3 proteinsRecent structural studiesBase editing technologyEnzyme catalytic polypeptideProtein functionSubstrate recognitionDNA bindingEditing technologySsDNA recognitionSubstrate selectionNovel CRISPRDeamination activityApolipoprotein B mRNACatalytic polypeptideBiochemical levelBase editorsLoop regionInnate immune systemProteinA3ADeaminase activityA3GA3 family
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