7b. Two Group T-test Comparisons
Generate two separate .msb files for each group as seen in the Multisubject Manual Page.
- Choose dualmultisubject from the BioImageSuite main menu. Three windows will appear: a Transform Viewer, a Reference Viewer and a BrainRegister menu bar. On the BrainRegister menu bar choose the first (Multisubject | Base). A new MultiSubject Tool window will appear.
- On the new MultiSubject Tool window choose (Setup | Load Setup).
- Choose your generated .msb file for your first group and click Open.
- On the MultiSubject Tool window choose the Results Tab. Choose your task of interest from the list of tasks then click Change Current Task.
- On the MultiSubject Tool window click (Images | Load All Images).
- On the MultiSubject Tool window click Compute Average Functional.
- On the BrainRegister menu bar choose the second (Multisubject2 | Base). A second MultiSubject Tool window will appear.
- On the second MultiSubject Tool window choose (Setup | Load Setup).
- Choose your generated .msb file for your second group and click Open.
- On the MultiSubject Tool window choose the Results Tab. Choose your task of interest from the list of tasks then click Change Current Task.
- On the MultiSubject Tool window click (Images | Load All Images).
- On the MultiSubject Tool window click Compute Average Functional.
- On either Multisubject window click the Base tab and under the Reference window click Display Reference.
- On either Multisubject window under Comparisons click Show Compare Tool. A new Registration/Overlay Tool window will appear and it will be on the Compare Image tab. The filename boxes will be empty.
- From the first Multisubject window (Group 1) under Comparisons click Send Mean Functional to Compare Tool as Set1. This will fill in the first filename box on the Registration/Overlay Tool window.
- From the second Multisubject window (Group 2) under Comparisons click Send Mean Functional to Compare Tool as Set2. This will fill in the second filename box on the Registration/Overlay Tool window.
- On the Registration/Overly Tool window click Compute Tmap. The computed functional image will be displayed in the transform window.
- On the Registration/Overlay Tool window click the Functional Overlay tab.
- Choose your threshold and enter it into the Low Threshold box. Refer to instructions for 5a (steps 4-8) for an explanation on how to choose a threshold.
- On the Registration/Overlay Tool window click Create Overlay.