In-Hyun Park PhD
Assistant Professor of Genetics; Yale Stem Cell Center
Reprogramming; iPS cells; Neurodevelopment; Epigenetics
- Investigation of genetic and epigenetic regulation of reprogramming
- in vitro model of human neurodevelopmental disease
Overexpression of four factors (Oct4, Sox2, Klf4, Myc, or
Oct4, Sox2, Nanog, Lin28) reprogram somatic cells to become induced pluripotent
stem (iPS) cells. Reprogramming accompanies genetic and epigenetic changes, and
its molecular mechanism is still unknown. We recently showed that in iPS cells
the global DNA methylation status is close to that of human embryonic stem
(hES) cells, suggesting the epigenetic resetting during reprogramming.
Furthermore, we have showed the possible dissection of stages in reprogramming
through live cell imaging analysis.
We will investigate the molecular mechanism of genetic and
epigenetic change during reprogramming. iPS cells show similar characteristics
as hES cells, such as self-renewal and pluripotency, and provide an incredible
resource for cell-based therapy, in vitro disease model and screening drugs.
- Kim, K.-Y., E. Hysolli, Y. Tanaka, B. Wang, Y.-W. Jung, X. Pan, S. M. Weissman, and I.-H. Park (2014) X Chromosome of Female Cells Shows Dynamic Changes in Status during Human Somatic Cell Reprogramming. Stem Cell Reports, 2:896-909.
- Tanaka, Y., K. Y. Kim, M. Zhong, X. Pan, S. M. Weissman, and I. H. Park (2014) Transcriptional regulation in pluripotent stem cells by methyl CpG-binding protein 2 (MeCP2). Hum Mol Genet, 23:1045-55.
- Tulpule, A., J. M. Kelley, M. W. Lensch, J. McPherson, I. H. Park, O. Hartung, T. Nakamura, T. M. Schlaeger, A. Shimamura and G. Q. Daley (2013). Pluripotent stem cell models of shwachman-diamond syndrome reveal a common mechanism for pancreatic and hematopoietic dysfunction. Cell Stem Cell 12: 727-736.
- Kim, K. Y., Y. W. Jung, G. J. Sullivan, L. Chung and I. H. Park (2012). Cellular reprogramming: a novel tool for investigating autism spectrum disorders. Trends Mol Med 18: 463-471.
- Kun-Yong Kim, Eriona Hysolli, In-Hyun Park. Neuronal maturation defect in induced pluripotent stem cells from Rett syndrome patients. Proc. Natl. Acad. Sci. 2011;108:14169-14174. (PMC3161557)
- Agarwal, S., Y. H. Loh, E. M. McLoughlin, J. Huang, I. H. Park, J. D. Miller, H. Huo, M. Okuka, R. M. Dos Reis, S. Loewer, H. H. Ng, D. L. Keefe, et al. (2010). Telomere elongation in induced pluripotent stem cells from dyskeratosis congenita patients. Nature 464: 292-296.
- Elayne M Chan*, Sutheera Ratanasirintrawoot*, In-Hyun Park*, Hongguang Huo, Philip D Manos, Junsung Rho, Yuin-Han Loh George Q. Daley and Thorsten M. Schlaeger (2009) Live cell imaging distinguishes bona fide human iPS cells from partially reprogrammed cells. Nature Biotechnology. 27:1033-1037. (* contributed equally)
- Akiko Doi*, In-Hyun Park*, Bo Wen*, Peter Murakami, Dr. Rafael Irizarry, Brian Herb, Sabine Loewer, Thorsten Schlaeger, Justine Miller, Junsung Rho, George Q. Daley, Andrew P. Feinberg (2009), Hypo- and Hypermethylation of Tissue- and Cancer-related CpG Island Shores in Human Induced Pluripotent Stem (iPS) Cells. Nature Genetics. 41:1350-1353. (* contributed equally)
- In-Hyun Park, Natasha Arora , Hongguang Huo, Nimet Maherali, Tim Ahfeldt, Akiko Shimamura, M. William Lensch, Chad Cowan, Konrad Hochedlinger and George Q Daley (2008) Disease-specific induced pluripotent stem cells. Cell, 134:877-886.