2020
Deficit in voluntary wheel running in chronic inflammatory and neuropathic pain models in mice: Impact of sex and genotype
Contreras KM, Caillaud M, Neddenriep B, Bagdas D, Roberts JL, Ulker E, White AB, Aboulhosn R, Toma W, Khalefa T, Adel A, Mann JA, Damaj MI. Deficit in voluntary wheel running in chronic inflammatory and neuropathic pain models in mice: Impact of sex and genotype. Behavioural Brain Research 2020, 399: 113009. PMID: 33181181, PMCID: PMC8961431, DOI: 10.1016/j.bbr.2020.113009.Peer-Reviewed Original ResearchConceptsNeuropathic pain modelChronic constriction injuryMechanical withdrawal thresholdPain modelStrains of miceWithdrawal thresholdDBA/2J miceVoluntary wheelChronic neuropathic pain modelPaclitaxel-treated miceVehicle-treated miceUnilateral intraplantar injectionLower mechanical thresholdsChronic pain reportQuality of lifeChemotherapy agent paclitaxelImpact of sexDifferent mouse strainsCCI miceCCI surgeryConstriction injuryIntraplantar injectionSham surgeryMale C57BL/6JNovel analgesicsImpact of menthol on nicotine intake and preference in mice: Concentration, sex, and age differences
Bagdas D, Jackson A, Carper M, Chen RY, Akinola LS, Damaj MI. Impact of menthol on nicotine intake and preference in mice: Concentration, sex, and age differences. Neuropharmacology 2020, 179: 108274. PMID: 32827516, PMCID: PMC7572603, DOI: 10.1016/j.neuropharm.2020.108274.Peer-Reviewed Original ResearchConceptsOral nicotine consumptionEffect of mentholImpact of mentholNicotine consumptionFemale miceNicotine intakeΑ7 nicotinic acetylcholine receptorMenthol concentrationNicotine solutionHigher nicotine intakeAdolescent female miceMale C57BL/6J miceTwo-bottle choice paradigmWild-type miceNicotinic acetylcholine receptorsConcentration-dependent mannerOral nicotineC57BL/6J miceKO miceMale miceType miceMouse modelAcetylcholine receptorsHigh menthol concentrationAdult counterparts
2019
Pharmacological mechanisms of alcohol analgesic-like properties in mouse models of acute and chronic pain
Neddenriep B, Bagdas D, Contreras K, Ditre J, Wolstenholme J, Miles M, Damaj M. Pharmacological mechanisms of alcohol analgesic-like properties in mouse models of acute and chronic pain. Neuropharmacology 2019, 160: 107793. PMID: 31562845, PMCID: PMC6924270, DOI: 10.1016/j.neuropharm.2019.107793.Peer-Reviewed Original ResearchConceptsAnalgesic-like propertiesComplete Freund's adjuvantAnti-nociceptive propertiesChronic painNeuropathic painChronic constriction injury modelAlcohol useMu-opioid systemOral ethanol administrationPain-like statePeripheral neuropathic painNeuropathic pain modelKappa-selective antagonistAcute alcohol useAnalgesic-like effectsDose-dependent reversalMechanical hypersensitivityAnalgesic effectNerve injuryPain modelAcute modelOpioid systemAcute alcoholFreund's adjuvantChronic useNeuropathic insult increases the responsiveness to acetic acid in mice.
Gurdap C, Markwalter P, Neddenriep B, Bagdas D, Damaj M. Neuropathic insult increases the responsiveness to acetic acid in mice. Behavioural Pharmacology 2019, 30: 534-537. PMID: 31033524, PMCID: PMC6684379, DOI: 10.1097/fbp.0000000000000486.Peer-Reviewed Original ResearchConceptsChronic constriction injuryChronic neuropathic painNeuropathic painPlace aversionAcute visceral painVehicle-treated micePeripheral nerve injuryPlace aversion (CPA) testPaclitaxel-treated animalsMillions of patientsCCI miceConstriction injuryVisceral painCCI animalsSham miceAcute painNerve injuryControl miceSciatic nerveNeuropathic insultMale miceNociceptive modelsPaclitaxel treatmentPainPlace conditioningAdolescent but not adult ethanol binge drinking modulates ethanol behavioral effects in mice later in life
Younis R, Wolstenholme J, Bagdas D, Bettinger J, Miles M, Damaj M. Adolescent but not adult ethanol binge drinking modulates ethanol behavioral effects in mice later in life. Pharmacology Biochemistry And Behavior 2019, 184: 172740. PMID: 31326461, PMCID: PMC6697373, DOI: 10.1016/j.pbb.2019.172740.Peer-Reviewed Original ResearchConceptsEthanol intakeEthanol drinkingEthanol's behavioral effectsEarly exposureAdolescent ethanol exposureEnhanced ethanol intakeAlcohol use disorderAnxiety-related behaviorAdolescence altersDrug-taking behaviorAlcohol intakeC57BL/6J miceEthanol exposureWithdrawal stateLong-term effectsNeurochemical mechanismsMouse modelBinge drinkingAdult miceUse disordersSerious illnessAbuse liabilityDrinking paradigmAdult exposureAdolescent populationThe α7 nicotinic receptor silent agonist R-47 prevents and reverses paclitaxel-induced peripheral neuropathy in mice without tolerance or altering nicotine reward and withdrawal
Toma W, Kyte SL, Bagdas D, Jackson A, Meade JA, Rahman F, Chen ZJ, Del Fabbro E, Cantwell L, Kulkarni A, Thakur GA, Papke RL, Bigbee JW, Gewirtz DA, Damaj MI. The α7 nicotinic receptor silent agonist R-47 prevents and reverses paclitaxel-induced peripheral neuropathy in mice without tolerance or altering nicotine reward and withdrawal. Experimental Neurology 2019, 320: 113010. PMID: 31299179, PMCID: PMC6708482, DOI: 10.1016/j.expneurol.2019.113010.Peer-Reviewed Original ResearchConceptsChemotherapy-induced peripheral neuropathyPeripheral neuropathyNicotine rewardPaclitaxel treatmentRewarding effectsTreatment of CIPNPaclitaxel-induced mechanical hypersensitivityTumor-bearing NSG micePaclitaxel-induced peripheral neuropathyNon-small cell lung cancer cell linesCell lung cancer cell linesA549 non-small cell lung cancer cell lineMecamylamine-precipitated withdrawalAntitumor activityIntraepidermal nerve fibersLung cancer cell linesLung tumor growthNSCLC cell viabilityTumor-bearing miceIntrinsic rewarding effectsPlace preference testCancer cell linesConditioned place preference testMechanical hypersensitivityAgonist RC57BL/6 substrain differences in inflammatory and neuropathic nociception and genetic mapping of a major quantitative trait locus underlying acute thermal nociception
Bryant C, Bagdas D, Goldberg L, Khalefa T, Reed E, Kirkpatrick S, Kelliher J, Chen M, Johnson W, Mulligan M, Damaj M. C57BL/6 substrain differences in inflammatory and neuropathic nociception and genetic mapping of a major quantitative trait locus underlying acute thermal nociception. Molecular Pain 2019, 15: 1744806918825046. PMID: 30632432, PMCID: PMC6365993, DOI: 10.1177/1744806918825046.Peer-Reviewed Original ResearchConceptsAcute thermal nociceptionThermal nociceptionNerve injuryNociceptive behaviorPain modalitiesB6N miceAdjuvant modelComplete Freund's adjuvant (CFA) modelChronic constrictive nerve injuryBaseline mechanical thresholdInflammatory nociceptive behaviorsInflammatory nociceptive stimulusStrain differencesFreund's adjuvant modelDifferent pain modalitiesHot plate testPreclinical pain modelsHot plate sensitivityNeuropathic nociceptionNociceptive markersPaw diameterMechanical hypersensitivityInflammatory painNeuropathic painPain model
2018
Blockade of nicotinic acetylcholine receptor enhances the responsiveness to bupropion in the mouse forced swim test
Bagdas D, AlSharari S, Roni M, Campbell V, Muldoon P, Carroll F, Damaj M. Blockade of nicotinic acetylcholine receptor enhances the responsiveness to bupropion in the mouse forced swim test. Behavioural Brain Research 2018, 360: 262-269. PMID: 30552947, PMCID: PMC6324988, DOI: 10.1016/j.bbr.2018.12.027.Peer-Reviewed Original ResearchConceptsAntidepressant-like effectsImmobility timeWT miceDecreased immobility timeDihydro-β-erythroidineAdult male miceWild-type miceEffects of β2Nicotinic acetylcholine receptorsBaseline immobilityAntidepressant effectsPresent studyJ miceKO miceSwim testMale miceWT littermatesRole of α4Bupropion administrationBupropionAcute dosesFST behaviorTransgenic miceAcetylcholine receptorsNAChR subunitsKnockout of alpha 5 nicotinic acetylcholine receptors subunit alters ethanol-mediated behavioral effects and reward in mice
Dawson A, Wolstenholme JT, Roni MA, Campbell VC, Jackson A, Slater C, Bagdas D, Perez EE, Bettinger JC, De Biasi M, Miles MF, Damaj MI. Knockout of alpha 5 nicotinic acetylcholine receptors subunit alters ethanol-mediated behavioral effects and reward in mice. Neuropharmacology 2018, 138: 341-348. PMID: 29944862, PMCID: PMC6400055, DOI: 10.1016/j.neuropharm.2018.06.031.Peer-Reviewed Original ResearchConceptsΑ5 nAChREthanol consumptionΑ5 nicotinic acetylcholine receptor (nAChR) subunitsAlcohol dependenceBehavioral effectsImportance of nAChRsΑ5 nAChR subunitΑ5-KO miceNicotinic acetylcholine receptor subunitsEthanol-induced hypothermiaAnxiolytic-like responseEthanol drinking behaviorVoluntary ethanol consumptionNicotinic acetylcholine receptorsΑ5 knockout miceAcetylcholine receptor subunitsTwo-bottle choiceDecreases ethanol intakePlace preference assayEthanol-induced behaviorsWild-type controlsKO miceRestraint stressDID paradigmEthanol intakeNew insights on the effects of varenicline on nicotine reward, withdrawal and hyperalgesia in mice
Bagdas D, Alkhlaif Y, Jackson A, Carroll FI, Ditre JW, Damaj MI. New insights on the effects of varenicline on nicotine reward, withdrawal and hyperalgesia in mice. Neuropharmacology 2018, 138: 72-79. PMID: 29860196, PMCID: PMC6054891, DOI: 10.1016/j.neuropharm.2018.05.025.Peer-Reviewed Original ResearchConceptsEffects of vareniclineNicotine withdrawal signsNicotine rewardΑ5 nAChRWithdrawal signsHigh doseKnockout miceΒ2-nAChRsNicotine withdrawal-induced hyperalgesiaAdministration of vareniclineWithdrawal-induced hyperalgesiaΑ7 knockout miceDose-related mannerNicotinic acetylcholine receptorsΑ5 knockout micePlace preference testVarenicline doseCessation treatmentNicotine withdrawalSomatic signsVareniclineΑ7 nAChRsMouse modelCPP testNicotinic subtypes
2017
Nicotine prevents and reverses paclitaxel-induced mechanical allodynia in a mouse model of CIPN
Kyte S, Toma W, Bagdas D, Meade J, Schurman L, Lichtman A, Chen Z, Del Fabbro E, Fang X, Bigbee J, Damaj M, Gewirtz D. Nicotine prevents and reverses paclitaxel-induced mechanical allodynia in a mouse model of CIPN. Journal Of Pharmacology And Experimental Therapeutics 2017, 364: jpet.117.243972. PMID: 29042416, PMCID: PMC5738719, DOI: 10.1124/jpet.117.243972.Peer-Reviewed Original ResearchConceptsChemotherapy-induced peripheral neuropathyPaclitaxel-induced mechanical allodyniaMechanical allodyniaPeripheral neuropathyMouse modelTreatment of CIPNLewis lung carcinoma tumor growthIntraepidermal nerve fiber lossPaclitaxel-induced peripheral neuropathyH460 non-small cell lung cancer cellsNon-small cell lung cancer cellsLung tumor cell proliferationNerve fiber dysfunctionNicotinic acetylcholine receptor subtypesCell lung cancer cellsChronic nicotine administrationNerve fiber lossChronic nicotine treatmentMale C57BL/6J miceAcetylcholine receptor subtypesLung cancer cellsProliferation of A549Receptor-mediated pathwayTumor cell proliferationCIPN treatmentAssessment of nicotine withdrawal-induced changes in sucrose preference in mice
Alkhlaif Y, Bagdas D, Jackson A, Park AJ, Damaj IM. Assessment of nicotine withdrawal-induced changes in sucrose preference in mice. Pharmacology Biochemistry And Behavior 2017, 161: 47-52. PMID: 28919072, PMCID: PMC6408212, DOI: 10.1016/j.pbb.2017.08.013.Peer-Reviewed Original ResearchMeSH KeywordsAnhedoniaAnimalsMaleMiceMice, Inbred C57BLMice, KnockoutNicotineNicotinic AgonistsSubstance Withdrawal SyndromeSucroseTobacco Use DisorderConceptsSucrose preference testNicotine withdrawalSpontaneous nicotine withdrawalLight-dark box testDark box testPositive affective stimuliSucrose preferenceAffective signsSmoking relapse ratesΑ6 KO miceSubcutaneous osmotic minipumpsWithdrawal-induced changesUnderlying neurobiological factorsPreference testRelapse rateOsmotic minipumpsKO miceTobacco useNicotine dependenceKnockout miceAnimal modelsNicotinic subunitsDay 15MiceDifferent dosesEffects of paclitaxel on the development of neuropathy and affective behaviors in the mouse
Toma W, Kyte S, Bagdas D, Alkhlaif Y, Alsharari S, Lichtman A, Chen Z, Del Fabbro E, Bigbee J, Gewirtz D, Damaj M. Effects of paclitaxel on the development of neuropathy and affective behaviors in the mouse. Neuropharmacology 2017, 117: 305-315. PMID: 28237807, PMCID: PMC5489229, DOI: 10.1016/j.neuropharm.2017.02.020.Peer-Reviewed Original ResearchConceptsCancer chemotherapeutic drugsCancer patientsPreclinical modelsSide effectsAffective symptomsMajor dose-limiting side effectDose-limiting side effectChemotherapeutic drugsLight/dark box testNerve fiber dysfunctionPaclitaxel-treated miceProgression-free survivalSucrose preference testDepression-like behaviorOvarian cancer patientsSevere side effectsAnxiety-like behaviorPotential therapeutic interventionsEffect of paclitaxelAnhedonia-like stateDark box testCold allodyniaNeuropathic painNegative affective symptomsNociceptive effects
2016
The α7 nicotinic receptor dual allosteric agonist and positive allosteric modulator GAT107 reverses nociception in mouse models of inflammatory and neuropathic pain
Bagdas D, Wilkerson J, Kulkarni A, Toma W, AlSharari S, Gul Z, Lichtman A, Papke R, Thakur G, Damaj M. The α7 nicotinic receptor dual allosteric agonist and positive allosteric modulator GAT107 reverses nociception in mouse models of inflammatory and neuropathic pain. British Journal Of Pharmacology 2016, 173: 2506-2520. PMID: 27243753, PMCID: PMC4959951, DOI: 10.1111/bph.13528.Peer-Reviewed Original ResearchConceptsPositive allosteric modulatorsNeuropathic painPain modelAntinociceptive effectSpinal cordTail flickChronic constriction injury (CCI) neuropathic pain modelAllosteric agonistDose-dependent antinociceptive effectΑ7 nicotinic ACh receptorsGlial fibrillary acidic proteinNeuropathic pain modelAstrocyte-specific glial fibrillary acidic proteinInflammatory pain modelAcetic acid injectionHot-plate assayEffective pharmacological strategiesNicotinic ACh receptorsNovel therapeutic approachesFibrillary acidic proteinDorsal hornFormalin testPain modulationSubchronic administrationLocus of actionDiacylglycerol lipase β inhibition reverses nociceptive behaviour in mouse models of inflammatory and neuropathic pain
Wilkerson J, Ghosh S, Bagdas D, Mason B, Crowe M, Hsu K, Wise L, Kinsey S, Damaj M, Cravatt B, Lichtman A. Diacylglycerol lipase β inhibition reverses nociceptive behaviour in mouse models of inflammatory and neuropathic pain. British Journal Of Pharmacology 2016, 173: 1678-1692. PMID: 26915789, PMCID: PMC4842918, DOI: 10.1111/bph.13469.Peer-Reviewed Original ResearchConceptsChronic constrictive injuryNeuropathic pain modelWild-type miceAllodynic responsesInflammatory painPain modelLPS modelSide effectsExpression of LPSDiscernible side effectsUntoward side effectsPro-inflammatory responseEvidence of toleranceSites of inflammationConstrictive injuryMechanical allodyniaIntraplantar injectionNeuropathic painPathological painInflammatory mediatorsMouse peritoneal macrophagesLocus of actionNociceptive behaviorSciatic nerveAllodynia
2015
The role of alpha5 nicotinic acetylcholine receptors in mouse models of chronic inflammatory and neuropathic pain
Bagdas D, AlSharari S, Freitas K, Tracy M, Damaj M. The role of alpha5 nicotinic acetylcholine receptors in mouse models of chronic inflammatory and neuropathic pain. Biochemical Pharmacology 2015, 97: 590-600. PMID: 25931144, PMCID: PMC4600420, DOI: 10.1016/j.bcp.2015.04.013.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCarrageenanFormaldehydeFreund's AdjuvantInflammationMaleMiceMice, Inbred C57BLMice, KnockoutNeuralgiaReceptors, NicotinicConceptsΑ5-KO miceΑ5 nAChRInflammatory painFormalin testPain modelChronic painMouse modelTumor necrosis factor-alpha levelsLong-term inflammatory painNecrosis factor-alpha levelsPaw-licking timeTonic inflammatory painChronic pain modelsDevelopment of hyperalgesiaInflammatory pain modelWild-type miceNicotinic acetylcholine receptorsAllodynic responsesMechanical allodyniaNicotine reversalCFA injectionNeuropathic painAntinociceptive effectNociceptive behaviorPain