2021
Age-dependent ataxia and neurodegeneration caused by an αII spectrin mutation with impaired regulation of its calpain sensitivity
Miazek A, Zalas M, Skrzymowska J, Bogin BA, Grzymajło K, Goszczynski TM, Levine ZA, Morrow JS, Stankewich MC. Age-dependent ataxia and neurodegeneration caused by an αII spectrin mutation with impaired regulation of its calpain sensitivity. Scientific Reports 2021, 11: 7312. PMID: 33790315, PMCID: PMC8012654, DOI: 10.1038/s41598-021-86470-1.Peer-Reviewed Original ResearchConceptsSpectrin cleavageCalpain cleavage sitesCalcium-activated proteaseGlobal neurodegenerationTraumatic encephalopathyC57BL/6J miceDendritic integrityExcessive activationNeuronal integrityProgressive ataxiaImpaired regulationCalpain activationCalpain sensitivityPhysiologic significanceNeurodegenerative diseasesNeuronal developmentCalpain proteolysisCalpain proteasesCalcium-dependent bindingAtaxiaNeurodegenerationCalpainActivated calpainSubstrate-level regulationCaM affinity
2017
βIII Spectrin Is Necessary for Formation of the Constricted Neck of Dendritic Spines and Regulation of Synaptic Activity in Neurons
Efimova N, Korobova F, Stankewich MC, Moberly AH, Stolz DB, Wang J, Kashina A, Ma M, Svitkina T. βIII Spectrin Is Necessary for Formation of the Constricted Neck of Dendritic Spines and Regulation of Synaptic Activity in Neurons. Journal Of Neuroscience 2017, 37: 6442-6459. PMID: 28576936, PMCID: PMC5511878, DOI: 10.1523/jneurosci.3520-16.2017.Peer-Reviewed Original ResearchConceptsDendritic spinesSynaptic activitySpinocerebellar ataxia type 5Spine neckAberrant spine morphologyMushroom-shaped spinesType 5Autosomal recessive cerebellar ataxia type 1Spiny synapsesPostsynaptic excitationMiniature EPSCsShaft synapsesDamage neuronsNeuronal pathologyCortical neuronsSomatodendritic compartmentPresynaptic terminalsNeurodegenerative syndromePostsynaptic structuresSpine headsNeuropsychiatric disordersSynaptic strengthType 1Spine morphologyBrain function
2014
A hierarchy of ankyrin-spectrin complexes clusters sodium channels at nodes of Ranvier
Ho TS, Zollinger DR, Chang KJ, Xu M, Cooper EC, Stankewich MC, Bennett V, Rasband MN. A hierarchy of ankyrin-spectrin complexes clusters sodium channels at nodes of Ranvier. Nature Neuroscience 2014, 17: 1664-1672. PMID: 25362473, PMCID: PMC4271271, DOI: 10.1038/nn.3859.Peer-Reviewed Original Research
2012
A Distal Axonal Cytoskeleton Forms an Intra-Axonal Boundary that Controls Axon Initial Segment Assembly
Galiano MR, Jha S, Ho TS, Zhang C, Ogawa Y, Chang KJ, Stankewich MC, Mohler PJ, Rasband MN. A Distal Axonal Cytoskeleton Forms an Intra-Axonal Boundary that Controls Axon Initial Segment Assembly. Cell 2012, 149: 1125-1139. PMID: 22632975, PMCID: PMC3361702, DOI: 10.1016/j.cell.2012.03.039.Peer-Reviewed Original Research
2011
Cell organization, growth, and neural and cardiac development require αII-spectrin
Stankewich MC, Cianci CD, Stabach PR, Ji L, Nath A, Morrow JS. Cell organization, growth, and neural and cardiac development require αII-spectrin. Journal Of Cell Science 2011, 124: 3956-3966. PMID: 22159418, PMCID: PMC3244980, DOI: 10.1242/jcs.080374.Peer-Reviewed Original ResearchMeSH KeywordsActinsAnimalsAnkyrinsAxonsBody PatterningCarrier ProteinsCell MembraneCell PolarityCell ProliferationCraniofacial AbnormalitiesEmbryo, MammalianEmbryonic DevelopmentFemaleFibroblastsGene DeletionHeart Defects, CongenitalMaleMiceMice, Inbred C57BLMicrofilament ProteinsNeural Tube DefectsNeuroepithelial CellsPhenotypeProtein StabilityPseudopodiaSpectrinConceptsΑII-spectrinSteady-state protein levelsΒIII spectrinEmbryonic day 12.5Tissue patterningRenal epithelial cellsEmbryonic lethalCortical actinOrgan developmentAnkyrin BExon trappingEmbryonic fibroblastsTranscriptional levelΒ-spectrinCardiac developmentCell organizationCell spreadingAxon formationNeural tubeHeterozygous animalsTargeted disruptionApical membraneNeuroepithelial cellsDay 12.5Cell morphologySchwann cell spectrins modulate peripheral nerve myelination
Susuki K, Raphael AR, Ogawa Y, Stankewich MC, Peles E, Talbot WS, Rasband MN. Schwann cell spectrins modulate peripheral nerve myelination. Proceedings Of The National Academy Of Sciences Of The United States Of America 2011, 108: 8009-8014. PMID: 21518878, PMCID: PMC3093478, DOI: 10.1073/pnas.1019600108.Peer-Reviewed Original ResearchConceptsSchwann cell cytoskeletonCell cytoskeletonΒII spectrinSubmembranous cytoskeletal proteinsNeuron-glia interactionsEfficient action potential propagationSchwann cellsMembrane proteinsCytoskeletal rearrangementsLoss of spectrinCytoskeletal proteinsNectin-like proteinsCell shapeContact sitesF-actinPeripheral nerve myelinationSpectrinPeripheral nerve developmentΑII-spectrinCytoskeletonProteinNerve myelinationNerve developmentMotor nervesCells
2010
Targeted deletion of βIII spectrin impairs synaptogenesis and generates ataxic and seizure phenotypes
Stankewich MC, Gwynn B, Ardito T, Ji L, Kim J, Robledo RF, Lux SE, Peters LL, Morrow JS. Targeted deletion of βIII spectrin impairs synaptogenesis and generates ataxic and seizure phenotypes. Proceedings Of The National Academy Of Sciences Of The United States Of America 2010, 107: 6022-6027. PMID: 20231455, PMCID: PMC2851889, DOI: 10.1073/pnas.1001522107.Peer-Reviewed Original ResearchConceptsBetaIII spectrinSpinocerebellar ataxia type 5Spectrin membrane skeletonTargeted gene disruptionAmino acid transportersExcitatory amino acid transportersImpairs synaptogenesisProtein traffickingSeizure disorderGene disruptionDark Purkinje cellsMembrane skeletonAlphaII-spectrinAcid transportersMechanistic basisPurkinje cellsMembrane channelsGlutamate receptor deltaIntracellular pathwaysSpectrin bindsSynaptic proteinsSpectrinReceptor deltaProteinGolgi profiles
2005
HSP70 binding modulates detachment of Na-K-ATPase following energy deprivation in renal epithelial cells
Riordan M, Sreedharan R, Wang S, Thulin G, Mann A, Stankewich M, Van Why S, Kashgarian M, Siegel NJ. HSP70 binding modulates detachment of Na-K-ATPase following energy deprivation in renal epithelial cells. American Journal Of Physiology. Renal Physiology 2005, 288: f1236-f1242. PMID: 15701813, DOI: 10.1152/ajprenal.00438.2004.Peer-Reviewed Original ResearchConceptsRenal epithelial cellsATP depletionMolecular chaperone Hsp70Binding of Hsp70Na-K-ATPaseFundamental cellular mechanismsRenal epithelial polarityCultured renal epithelial cellsEpithelial cellsHeat shock protein 70Protein clathrinEpithelial polarityMolecular chaperonesOverexpression of HSP70Chaperone Hsp70Shock protein 70Energy deprivationLLC-PK1 cellsStress proteinsMolecular mechanismsHSP bindingHSP70Cell lysatesCellular mechanismsATP turnover
2002
Fragmentation of the Golgi Apparatus A ROLE FOR βIII SPECTRIN AND SYNTHESIS OF PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE*
Siddhanta A, Radulescu A, Stankewich MC, Morrow JS, Shields D. Fragmentation of the Golgi Apparatus A ROLE FOR βIII SPECTRIN AND SYNTHESIS OF PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE*. Journal Of Biological Chemistry 2002, 278: 1957-1965. PMID: 12411436, DOI: 10.1074/jbc.m209137200.Peer-Reviewed Original ResearchRegulation of TGF-β ligand and receptor expression in neonatal rat lungs exposed to chronic hypoxia
Vicencio AG, Eickelberg O, Stankewich MC, Kashgarian M, Haddad GG. Regulation of TGF-β ligand and receptor expression in neonatal rat lungs exposed to chronic hypoxia. Journal Of Applied Physiology 2002, 93: 1123-1130. PMID: 12183510, DOI: 10.1152/japplphysiol.00031.2002.Peer-Reviewed Original ResearchConceptsRat lungChronic hypoxiaArrest of alveolarizationTGF-beta levelsPostnatal day 14 ratsBronchoalveolar lavage fluidTGF-beta receptor INeonatal rat lungSprague-Dawley ratsPostnatal day 14Day 14 ratsTGF-beta activityWk of lifeTGF-beta systemEffects of hypoxiaHypoxic adultsWestern blot analysisLavage fluidReceptor expressionLong-term effectsAdult ratsAlveolar epitheliumImmunohistochemical analysisModulatory effectsDay 14
1996
Occludin is a functional component of the tight junction
McCarthy K, Skare I, Stankewich M, Furuse M, Tsukita S, Rogers R, Lynch R, Schneeberger E. Occludin is a functional component of the tight junction. Journal Of Cell Science 1996, 109: 2287-2298. PMID: 8886979, DOI: 10.1242/jcs.109.9.2287.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiological Transport, ActiveCadherinsCell LineCell Membrane PermeabilityChickensDNA, ComplementaryDogsElectric ImpedanceFreeze FracturingGene ExpressionImmunohistochemistryIsopropyl ThiogalactosideMannitolMembrane ProteinsOccludinPhosphoproteinsTight JunctionsTransfectionZonula Occludens-1 ProteinConceptsTight junction assemblyTight junction strandsJunction assemblyD-thiogalactosideSteady state transepithelial electrical resistanceLac repressor geneTight junctionsJunction strandsTransepithelial electrical resistanceZO-1Western blotRepressor geneNeoR clonesPlasma membraneConfocal laser scanning microscopyContact sitesFreeze-fracture analysisMDCK cellsVesicular structuresAqueous poresCell surfaceLaser scanning microscopyJunction activityCell membraneE-cadherin