2017
Aging impairs both primary and secondary RIG-I signaling for interferon induction in human monocytes
Molony RD, Nguyen JT, Kong Y, Montgomery RR, Shaw AC, Iwasaki A. Aging impairs both primary and secondary RIG-I signaling for interferon induction in human monocytes. Science Signaling 2017, 10 PMID: 29233916, PMCID: PMC6429941, DOI: 10.1126/scisignal.aan2392.Peer-Reviewed Original ResearchConceptsType I IFNsI IFNsI interferonOlder adultsIFN inductionRetinoic acid-inducible gene IAcid-inducible gene IHealthy human donorsType I interferonRespiratory influenzaProinflammatory cytokinesVirus infectionType I IFN genesAdult monocytesAntiviral resistanceTranscription factor IRF8IFN responseHuman donorsMonocytesIncreased proteasomal degradationHuman monocytesYoung adultsIRF8 expressionIAV RNAInfected cellsReduced dynamic range of antiviral innate immune responses in aging
Molony RD, Malawista A, Montgomery RR. Reduced dynamic range of antiviral innate immune responses in aging. Experimental Gerontology 2017, 107: 130-135. PMID: 28822811, PMCID: PMC5815956, DOI: 10.1016/j.exger.2017.08.019.Peer-Reviewed Original ResearchMeSH KeywordsAgedAgingCytokinesDendritic CellsHumansImmunity, InnateInflammasomesMonocytesSignal TransductionConceptsInnate immune responseImmune responseAntiviral innate immune responseKey pattern recognition receptorsAltered cytokine responsePattern recognition receptorsAntiviral interferon responseAge-related changesInflammatory mediatorsCytokine responsesChronic inflammationImmune functionNotable impairmentViral infectionInnate immunityRecognition receptorsInterferon responseProgressive declineViral pathogensAverage life spanResponseWorldwide populationParadoxical stateLife spanInflammation
2016
Mx1 reveals innate pathways to antiviral resistance and lethal influenza disease
Pillai PS, Molony RD, Martinod K, Dong H, Pang IK, Tal MC, Solis AG, Bielecki P, Mohanty S, Trentalange M, Homer RJ, Flavell RA, Wagner DD, Montgomery RR, Shaw AC, Staeheli P, Iwasaki A. Mx1 reveals innate pathways to antiviral resistance and lethal influenza disease. Science 2016, 352: 463-466. PMID: 27102485, PMCID: PMC5465864, DOI: 10.1126/science.aaf3926.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAdultAgedAged, 80 and overAnimalsBacterial InfectionsCaspase 1CaspasesCaspases, InitiatorFemaleHumansImmunity, InnateInfluenza A virusInfluenza, HumanInterferon-betaMaleMembrane GlycoproteinsMiceMonocytesMyxovirus Resistance ProteinsNeutrophilsOrthomyxoviridae InfectionsRespiratory Tract InfectionsToll-Like Receptor 7Viral LoadYoung AdultConceptsBacterial burdenAntiviral resistanceNeutrophil-dependent tissue damageMyD88-dependent signalingAntiviral interferon productionCaspase-1/11IAV diseaseViral loadInfluenza diseaseOlder humansTissue damageInterferon productionInflammasome responseOlder adultsTLR7Vivo consequencesDiseaseMiceIAVBurdenMx geneHumansMonocytesMortalityInfluenza
2014
CyTOF supports efficient detection of immune cell subsets from small samples
Yao Y, Liu R, Shin MS, Trentalange M, Allore H, Nassar A, Kang I, Pober JS, Montgomery RR. CyTOF supports efficient detection of immune cell subsets from small samples. Journal Of Immunological Methods 2014, 415: 1-5. PMID: 25450003, PMCID: PMC4269324, DOI: 10.1016/j.jim.2014.10.010.Peer-Reviewed Original ResearchConceptsImmune cell subsetsCell subsetsImmune cell statesPatient biopsiesTranslational investigationsFlow cytometryClinical researchCellular analysisMass cytometryMultiple cell populationsCell populationsCytometryCyTOFSingle-cell analysisMultiparameter single cell analysisFluorescence cytometryFluorescence-based flow cytometryCell statesHuman diseasesMarkersTremendous detailBiopsyPathogenesisHuman monocytes have increased IFN-γ-mediated IL-15 production with age alongside altered IFN-γ receptor signaling
Lee N, Shin MS, Kang KS, Yoo SA, Mohanty S, Montgomery RR, Shaw AC, Kang I. Human monocytes have increased IFN-γ-mediated IL-15 production with age alongside altered IFN-γ receptor signaling. Clinical Immunology 2014, 152: 101-110. PMID: 24657713, PMCID: PMC4018768, DOI: 10.1016/j.clim.2014.03.003.Peer-Reviewed Original Research
2012
Quantitative imaging of lineage-specific Toll-like receptor-mediated signaling in monocytes and dendritic cells from small samples of human blood.
Qian F, Montgomery RR. Quantitative imaging of lineage-specific Toll-like receptor-mediated signaling in monocytes and dendritic cells from small samples of human blood. Journal Of Visualized Experiments 2012 PMID: 22525943, PMCID: PMC3466655, DOI: 10.3791/3741.Peer-Reviewed Original ResearchConceptsToll-like receptorsDendritic cellsImmune responseToll-like receptor-mediated signalingCell-mediated immune responsesCertain Toll-like receptorsAntigen-presenting cellsPeripheral blood monocytesHealthy human donorsNF-κB pathwayInnate immune systemReceptor-mediated signalingQuantitative flow cytometryTLR levelsWest Nile virusPatient susceptibilityUnderstanding of immunosenescenceImmune responsivenessElderly donorsLarge cohortOlder donorsAdaptive immunityBlood monocytesNF-κBTherapeutic interventions
2011
Age‐associated elevation in TLR5 leads to increased inflammatory responses in the elderly
Qian F, Wang X, Zhang L, Chen S, Piecychna M, Allore H, Bockenstedt L, Malawista S, Bucala R, Shaw AC, Fikrig E, Montgomery RR. Age‐associated elevation in TLR5 leads to increased inflammatory responses in the elderly. Aging Cell 2011, 11: 104-110. PMID: 22023165, PMCID: PMC3257374, DOI: 10.1111/j.1474-9726.2011.00759.x.Peer-Reviewed Original ResearchMeSH KeywordsAdultAgedAged, 80 and overAgingExtracellular Signal-Regulated MAP KinasesFemaleHumansInflammationInterleukin-8MaleMiddle AgedMonocytesMultivariate AnalysisNF-kappa BP38 Mitogen-Activated Protein KinasesPhosphorylationProtein TransportRNA, MessengerSignal TransductionToll-Like Receptor 5Tumor Necrosis Factor-alphaConceptsToll-like receptorsIL-8Multivariable mixed-effects modelsOlder individualsElevated IL-8Levels of TLR5Expression of TLR5Production of TNFAge-associated elevationAge-related decreaseDendritic cellsImmune responsivenessElderly donorsInflammatory responseImmune functionNF-κBTLR5Progressive declineMonocytesMixed effects modelsMAPK p38Significant increaseEffects modelAssociated increaseCritical mechanism
2010
Circulating monocytes from systemic sclerosis patients with interstitial lung disease show an enhanced profibrotic phenotype
Mathai SK, Gulati M, Peng X, Russell TR, Shaw AC, Rubinowitz AN, Murray LA, Siner JM, Antin-Ozerkis DE, Montgomery RR, Reilkoff RA, Bucala RJ, Herzog EL. Circulating monocytes from systemic sclerosis patients with interstitial lung disease show an enhanced profibrotic phenotype. Laboratory Investigation 2010, 90: 812-823. PMID: 20404807, PMCID: PMC3682419, DOI: 10.1038/labinvest.2010.73.Peer-Reviewed Original ResearchConceptsInterstitial lung diseaseSSc-ILD patientsSSc-ILDIL-10Normal controlsProfibrotic cellsSystemic sclerosisLung diseaseCollagen-producing cellsMCP-1Profibrotic phenotypeSSc-related interstitial lung diseaseFlow cytometryPeripheral blood profilesSSc-ILD cohortsIL-10 secretionSystemic sclerosis patientsExpression of CD163Blood of patientsHealthy aged controlsCultured CD14Profibrotic characteristicsProfibrotic mediatorsTNF levelsSclerosis patients
2002
Human phagocytic cells in the early innate immune response to Borrelia burgdorferi
Montgomery RR, Lusitani D, de Boisfleury Chevance A, Malawista SE. Human phagocytic cells in the early innate immune response to Borrelia burgdorferi. The Journal Of Infectious Diseases 2002, 185: 1773-1779. PMID: 12085324, DOI: 10.1086/340826.Peer-Reviewed Original ResearchConceptsPolymorphonuclear leukocytesImmune responseEarly innate immune responseKilling of spirochetesSpecific antibodiesBorrelia burgdorferiSecondary immune responseInnate immune responseInnate immune systemHuman phagocytic cellsSpirochete clearanceMononuclear cellsImmune systemLyme diseasePhagocytic cellsNatural infectionMature macrophagesSpirochetesIntracellular colocalizationAntibodiesBurgdorferiFirst cellsLimited uptakeCellsMonocytes
1986
Effects of reagent and cell-generated hydrogen peroxide on the properties of low density lipoprotein.
Montgomery RR, Nathan CF, Cohn ZA. Effects of reagent and cell-generated hydrogen peroxide on the properties of low density lipoprotein. Proceedings Of The National Academy Of Sciences Of The United States Of America 1986, 83: 6631-6635. PMID: 3018740, PMCID: PMC386558, DOI: 10.1073/pnas.83.17.6631.Peer-Reviewed Original ResearchConceptsLow-density lipoproteinDensity lipoproteinHuman monocytesModification of LDLUntreated low-density lipoproteinOxidation of LDLThiobarbituric acidAortic endothelial cell linePhorbol myristate acetateEndothelial cell lineReactive oxygen intermediatesCell line monolayersRespiratory burstMyristate acetateMacrophagesMouse macrophagesReagent H2O2Cell linesOxygen intermediatesMonocytesLipoproteinTBA reactivityHuman plasmaMaximal oxidation