2016
Adult human megakaryocyte-erythroid progenitors are in the CD34+CD38mid fraction
Sanada C, Xavier-Ferrucio J, Lu YC, Min E, Zhang PX, Zou S, Kang E, Zhang M, Zerafati G, Gallagher PG, Krause DS. Adult human megakaryocyte-erythroid progenitors are in the CD34+CD38mid fraction. Blood 2016, 128: 923-933. PMID: 27268089, PMCID: PMC4990855, DOI: 10.1182/blood-2016-01-693705.Peer-Reviewed Original ResearchConceptsMegakaryocyte/erythroid progenitorsComparative expression analysisNovel enrichment strategyMegakaryocyte-erythroid progenitorsPurification strategySingle-cell levelShort hairpin RNAFate decisionsE lineageNovel purification strategyLineage fateLineage commitmentGranulocyte colony-stimulating factor-mobilized peripheral bloodMK lineageExpression analysisE progenitorsErythroid lineageFactor-mobilized peripheral bloodDifferential expressionES cellsErythroid progenitorsMYB knockdownHairpin RNALineagesColony-forming unitsSetd1a and NURF mediate chromatin dynamics and gene regulation during erythroid lineage commitment and differentiation
Li Y, Schulz VP, Deng C, Li G, Shen Y, Tusi BK, Ma G, Stees J, Qiu Y, Steiner LA, Zhou L, Zhao K, Bungert J, Gallagher PG, Huang S. Setd1a and NURF mediate chromatin dynamics and gene regulation during erythroid lineage commitment and differentiation. Nucleic Acids Research 2016, 44: 7173-7188. PMID: 27141965, PMCID: PMC5009724, DOI: 10.1093/nar/gkw327.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigens, NuclearCell LineageCells, CulturedChromatinChromatin Assembly and DisassemblyChromatin ImmunoprecipitationErythroblastsErythrocyte CountErythrocytesErythropoiesisFemaleGene Expression RegulationHemoglobinsHistone-Lysine N-MethyltransferaseHistonesHumansLysineMaleMethylationMiceMice, KnockoutMicrococcal NucleaseMultiprotein ComplexesNerve Tissue ProteinsPromoter Regions, GeneticSpleenTranscription FactorsUpstream Stimulatory FactorsConceptsNURF complexChromatin dynamicsErythroid genesLineage commitmentAdult β-globin geneErythroid gene promotersErythroid lineage differentiationCell context-dependent mannerErythroid lineage commitmentChromatin structural alterationsContext-dependent mannerΒ-globin geneChromatin architectureEnhancer accessibilityChromatin accessibilityNucleosome repositioningTranscription regulationChromatin structureH3K4 methylationGene regulationComplex occupancyMammalian cellsGene activationGene transcriptionLineage differentiation
2013
USF1 and hSET1A Mediated Epigenetic Modifications Regulate Lineage Differentiation and HoxB4 Transcription
Deng C, Li Y, Liang S, Cui K, Salz T, Yang H, Tang Z, Gallagher PG, Qiu Y, Roeder R, Zhao K, Bungert J, Huang S. USF1 and hSET1A Mediated Epigenetic Modifications Regulate Lineage Differentiation and HoxB4 Transcription. PLOS Genetics 2013, 9: e1003524. PMID: 23754954, PMCID: PMC3675019, DOI: 10.1371/journal.pgen.1003524.Peer-Reviewed Original ResearchMeSH KeywordsBasic Helix-Loop-Helix Transcription FactorsCell DifferentiationCell LineageEmbryonic Stem CellsEpigenesis, GeneticGene Expression RegulationHistone-Lysine N-MethyltransferaseHomeodomain ProteinsHumansK562 CellsMesodermMethyltransferasesProto-Oncogene ProteinsT-Cell Acute Lymphocytic Leukemia Protein 1Transcription FactorsTranscriptional ActivationUpstream Stimulatory FactorsConceptsEmbryonic stem cellsLineage differentiationTrithorax complexesHoxb4 genesMethyltransferase complexTranscription preinitiation complex assemblyHistone H3K4 methyltransferase complexRNA interference-mediated knockdownBivalent chromatin domainsH3K4 methyltransferase complexUndifferentiated embryonic stem cellsPreinitiation complex assemblyHistone methyltransferase complexTranscription factor USF1H3K4 methyltransferase activityHematopoietic stem/progenitor cellsHematopoietic cell populationsStem/progenitor cellsDPY-30HOXB4 promoterMesoderm specificationChromatin domainsMesoderm differentiationH3K4me3 modificationHematopoietic transition
2012
Altered subcellular localization of transcription factor TEAD4 regulates first mammalian cell lineage commitment
Home P, Saha B, Ray S, Dutta D, Gunewardena S, Yoo B, Pal A, Vivian JL, Larson M, Petroff M, Gallagher PG, Schulz VP, White KL, Golos TG, Behr B, Paul S. Altered subcellular localization of transcription factor TEAD4 regulates first mammalian cell lineage commitment. Proceedings Of The National Academy Of Sciences Of The United States Of America 2012, 109: 7362-7367. PMID: 22529382, PMCID: PMC3358889, DOI: 10.1073/pnas.1201595109.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBlastocystBlastocyst Inner Cell MassBlastomeresBlotting, WesternCattleCDX2 Transcription FactorCell LineageCell NucleusCells, CulturedDNA-Binding ProteinsEmbryonic Stem CellsGATA3 Transcription FactorGene Expression Regulation, DevelopmentalGreen Fluorescent ProteinsHEK293 CellsHomeodomain ProteinsHumansMacaca mulattaMiceMice, TransgenicMuscle ProteinsRatsReverse Transcriptase Polymerase Chain ReactionRNA InterferenceTEA Domain Transcription FactorsTranscription FactorsConceptsInner cell massTranscriptional programsICM lineagesSubcellular localizationNuclear localizationInner blastomeresCell fate specificationSpecific transcriptional programsCell lineage commitmentAltered subcellular localizationTranscription factor TEAD4Preimplantation mouse embryosFate specificationLineage commitmentTarget genesMouse embryosCell lineagesTEAD4LineagesBlastomeresBlastocyst formationCell massDifferential functionGenesLocalization
2011
Single-lineage transcriptome analysis reveals key regulatory pathways in primitive erythroid progenitors in the mouse embryo
Isern J, He Z, Fraser ST, Nowotschin S, Ferrer-Vaquer A, Moore R, Hadjantonakis AK, Schulz V, Tuck D, Gallagher PG, Baron MH. Single-lineage transcriptome analysis reveals key regulatory pathways in primitive erythroid progenitors in the mouse embryo. Blood 2011, 117: 4924-4934. PMID: 21263157, PMCID: PMC3100699, DOI: 10.1182/blood-2010-10-313676.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceCell LineageCytokinesDNA PrimersEpsilon-GlobinsErythroid Precursor CellsErythropoiesisFemaleGene Expression ProfilingGene Expression Regulation, DevelopmentalGene Regulatory NetworksGlycolysisGreen Fluorescent ProteinsGrowth SubstancesMaleMiceMice, Inbred ICRMice, TransgenicOxygenPregnancyRecombinant Fusion ProteinsRNA, MessengerSignal TransductionConceptsPrimitive erythroid progenitorsMouse embryosErythroid progenitorsGlobal expression profilesEmbryonic day 7.5Critical regulatory factorKey regulatory pathwaysOnset of circulationFirst transcriptomeRemarkable proliferative capacityTranscript diversityTransgenic reporterTranscriptome analysisFirst cell typeRegulatory pathwaysHematopoietic lineagesExpression profilesRegulatory factorsCell typesDay 7.5EmbryosProgenitorsYolk sacBlood progenitorsGlycolytic profile