2014
Piezo Proteins: Regulators of Mechanosensation and Other Cellular Processes*
Bagriantsev SN, Gracheva EO, Gallagher PG. Piezo Proteins: Regulators of Mechanosensation and Other Cellular Processes*. Journal Of Biological Chemistry 2014, 289: 31673-31681. PMID: 25305018, PMCID: PMC4231648, DOI: 10.1074/jbc.r114.612697.Peer-Reviewed Original ResearchConceptsPiezo proteinsCellular processesMammalian cellsCellular developmentMechanosensory transductionCellular migrationIon channelsHereditary xerocytosisVolume regulationProteinBiologic processesRegulationImportant insightsTransductionMechanosensationRegulatorMutationsXerocytosisProliferationCellsMechanoVariety of disordersElongationMigrationProminent feature
2013
Disorders of red cell volume regulation
Gallagher PG. Disorders of red cell volume regulation. Current Opinion In Hematology 2013, 20: 201-207. PMID: 23519154, DOI: 10.1097/moh.0b013e32835f6870.Peer-Reviewed Original ResearchConceptsVolume regulationCassette family memberErythrocyte volume homeostasisErythrocyte hydrationCell volume regulationMechanosensory proteinsGenetic basisSolute homeostasisMechanotransduction pathwaysGlucose transporterNew therapeutic targetsErythrocyte volume regulationNovel mechanismCellular waterCellular dehydrationRecent insightsCellular volumeHomeostasisRegulationAnion transportersTherapeutic targetTransportersPathwayVolume homeostasisRecent studies
2009
Sites of Regulated Phosphorylation that Control K-Cl Cotransporter Activity
Rinehart J, Maksimova Y, Tanis J, Stone K, Hodson C, Zhang J, Risinger M, Pan W, Wu D, Colangelo C, Forbush B, Joiner C, Gulcicek E, Gallagher P, Lifton R. Sites of Regulated Phosphorylation that Control K-Cl Cotransporter Activity. Journal Of End-to-End-testing 2009, 138: 525-536. DOI: 10.1016/s9999-9994(09)20441-7.Peer-Reviewed Original ResearchIntrinsic transport activityK-Cl cotransporterTransport activityCell volume regulationRegulated phosphorylationRNA interferenceAlanine substitutionsCultured cellsHomologous sitesKCC activityWNK1 expressionNeonatal mouse brainVolume regulationNeuronal functionHypotonic conditionsActive cotransportPhosphorylationIntracellular chloride concentrationCotransporter activityKCC3Human red blood cellsKCC2 activationFundamental roleMouse brainRegulationSites of Regulated Phosphorylation that Control K-Cl Cotransporter Activity
Rinehart J, Maksimova YD, Tanis JE, Stone KL, Hodson CA, Zhang J, Risinger M, Pan W, Wu D, Colangelo CM, Forbush B, Joiner CH, Gulcicek EE, Gallagher PG, Lifton RP. Sites of Regulated Phosphorylation that Control K-Cl Cotransporter Activity. Cell 2009, 138: 525-536. PMID: 19665974, PMCID: PMC2811214, DOI: 10.1016/j.cell.2009.05.031.Peer-Reviewed Original ResearchConceptsIntrinsic transport activityK-Cl cotransporterTransport activityCell volume regulationRegulated phosphorylationRNA interferenceAlanine substitutionsCultured cellsHomologous sitesKCC activityCl exitWNK1 expressionNeonatal mouse brainVolume regulationNeuronal functionHypotonic conditionsActive cotransportPhosphorylationIntracellular chloride concentrationCotransporter activityKCC3Human red blood cellsKCC2 activationFundamental roleMouse brain
2007
A GC Box Element Is Critical for Transcriptional Regulation of the K-Cl Cotransporter Isoform KCC3a in Hematopoetic Cells.
Crable S, Joiner C, Gallagher P. A GC Box Element Is Critical for Transcriptional Regulation of the K-Cl Cotransporter Isoform KCC3a in Hematopoetic Cells. Blood 2007, 110: 1712. DOI: 10.1182/blood.v110.11.1712.1712.Peer-Reviewed Original ResearchUntranslated sequenceInitiator methionineErythroid cellsNovel transcriptsPromoter activityExon 1Cell RNATerminal truncationExon 1DTrans-regulatory factorsMajor splicing isoformsPotential phosphorylation sitesErythroid K562 cellsGC-box elementsFull promoter activityPrimer extension analysisTranslation initiation siteLate erythroid cellsFurther deletion analysisVolume regulationKCl cotransporterLuciferase promoter constructsBone marrow RNATranscriptional regulationEST database
2006
Alternative Splicing within Exon 1 of the KCl Cotransporter-3 (KCC3) Gene Results in Novel Transcripts in Erythroid Cells.
Joiner C, Crable S, Gallagher P. Alternative Splicing within Exon 1 of the KCl Cotransporter-3 (KCC3) Gene Results in Novel Transcripts in Erythroid Cells. Blood 2006, 108: 1564. DOI: 10.1182/blood.v108.11.1564.1564.Peer-Reviewed Original ResearchUntranslated sequenceNovel transcriptsInitiator methionineErythroid cellsAlternative splicingPromoter activityExon 1Cell RNATerminal truncationPotential phosphorylation sitesMajor splicing isoformsErythroid K562 cellsPrimer extension analysisTranslation initiation siteVolume regulationNegative regulatory elementKCl cotransporterLuciferase promoter constructsBone marrow RNAEST databaseTranscriptional controlGenomic regionsPhosphorylation sitesSplicing isoformsDifferent transcripts