2009
Genome Wide Profiling of USF1 and USF2 Occupancy in Human Primary Erythroid Cells.
Steiner L, Schulz V, Maksimova Y, Tuck D, Gallagher P. Genome Wide Profiling of USF1 and USF2 Occupancy in Human Primary Erythroid Cells. Blood 2009, 114: 1456. DOI: 10.1182/blood.v114.22.1456.1456.Peer-Reviewed Original ResearchTranscription start siteLocus control regionBeta-globin locus control regionGenome-wide mapsUSF proteinsPrimary erythroid cellsBeta-globin promoterHypersensitive site 2Erythroid cellsTranscription factorsE-boxDevelopmental stage-specific genesInsulator elementsUSF bindingHelix-leucine zipper proteinBeta-globin transcriptionErythroid-expressed genesE-box consensus motifsMammalian erythroid cellsGenome-wide profilingHuman primary erythroid cellsLeucine zipper proteinCanonical E-boxMRNA transcript analysisChIP-seq experiments
2008
Insulator Elements in Erythrocyte Membrane Genes
Steiner L, Maksimova Y, Lin J, Owen A, Schulz V, Bodine D, Gallagher P. Insulator Elements in Erythrocyte Membrane Genes. Blood 2008, 112: 3584. DOI: 10.1182/blood.v112.11.3584.3584.Peer-Reviewed Original ResearchErythrocyte membrane protein genesPosition-effect variegationMembrane protein geneProtein geneChromatin domainsAnkyrin promoterDNA sequencesErythroid cellsInsulator elementsMapping protein-DNA interactionsHeterochromatin-mediated geneGenome-wide scaleGenomic DNA microarrayBinding of CTCFStage-specific transcriptsEnhancer-blocking functionProtein-DNA interactionsCopy number-dependent expressionTissue-specific expressionProtein gene expressionCTCF occupancyRepetitive DNAInsulator functionSeparate enhancersErythroid promoter
2006
Lentivirus Vectors Containing a Band 3/γ-Globin Gene Flanked by Distinct Insulator Elements Are Resistant to Gene Silencing in Primary Mouse Erythroid Cells.
Harrow F, Frazar T, Seidel N, Gallagher P, Bodine D. Lentivirus Vectors Containing a Band 3/γ-Globin Gene Flanked by Distinct Insulator Elements Are Resistant to Gene Silencing in Primary Mouse Erythroid Cells. Blood 2006, 108: 3260. DOI: 10.1182/blood.v108.11.3260.3260.Peer-Reviewed Original ResearchΓ-globin geneLocus control regionInsulator elementsΓ-globinΓ-globin mRNAB3 promoterStart siteΓ-globin gene expressionMouse hematopoietic progenitor cellsLike globin genesAbundant membrane proteinErythroid-specific expressionTranscription start siteΓ-globin expressionMouse erythroid cellsCorrect start sitesHigh-throughput real-time PCRHypersensitive site 4Transgenic miceΒ-globin geneMultiple upstream sitesInsulator activityGlobin genesHematopoietic stem cellsMembrane proteins
2003
Variegated Expression from the Murine Band 3 (AE1) Promoter in Transgenic Mice Is Associated with mRNA Transcript Initiation at Upstream Start Sites and Can Be Suppressed by the Addition of the Chicken β-Globin 5′ HS4 Insulator Element
Frazar TF, Weisbein JL, Anderson SM, Cline AP, Garrett LJ, Felsenfeld G, Gallagher PG, Bodine DM. Variegated Expression from the Murine Band 3 (AE1) Promoter in Transgenic Mice Is Associated with mRNA Transcript Initiation at Upstream Start Sites and Can Be Suppressed by the Addition of the Chicken β-Globin 5′ HS4 Insulator Element. Molecular And Cellular Biology 2003, 23: 4753-4763. PMID: 12832463, PMCID: PMC162203, DOI: 10.1128/mcb.23.14.4753-4763.2003.Peer-Reviewed Original ResearchConceptsStart siteGamma-globin mRNAUpstream start siteVariegated expressionInsulator elementsHuman gamma-globin geneGamma-globin proteinPosition-effect variegationGamma-globin geneErythroid-specific expressionHS4 insulator elementsBeta-globin clusterHigh steady-state levelsTransgenic mouse assaysErythrocyte membrane skeletonTransgenic miceTransgene copy numberTranscript initiationCytoplasmic domainTransmembrane proteinSteady-state levelsRNA transcriptionMembrane skeletonGene promoterBeta spectrin