2011
Chromatin boundaries require functional collaboration between the hSET1 and NURF complexes
Li X, Wang S, Li Y, Deng C, Steiner LA, Xiao H, Wu C, Bungert J, Gallagher PG, Felsenfeld G, Qiu Y, Huang S. Chromatin boundaries require functional collaboration between the hSET1 and NURF complexes. Blood 2011, 118: 1386-1394. PMID: 21653943, PMCID: PMC3152501, DOI: 10.1182/blood-2010-11-319111.Peer-Reviewed Original ResearchConceptsErythroid genesInsulator sitesBarrier activityActive chromatin structureNucleosome remodeling activitiesChromatin barrier activityHistone H3K4 methyltransferaseChicken β-globinChromatin boundariesNURF complexChromatin structureInsulator functionNucleosome positioningMultiprotein complexesProtein complexesH3K4 methyltransferaseHS4 insulatorChromatin insulatorNURFH3K27me3 levelsLoci resultsLinker regionKnock-downΒ-globinFunctional collaboration
2010
A Common Regulatory Signature Associated with Barrier Insulators in Human Primary Erythroid Cells
Steiner L, Maksimova Y, Schulz V, Gallagher P. A Common Regulatory Signature Associated with Barrier Insulators in Human Primary Erythroid Cells. Blood 2010, 116: 3868. DOI: 10.1182/blood.v116.21.3868.3868.Peer-Reviewed Original ResearchGenome-wide mapsPrimary erythroid cellsErythroid cellsProximal promoterChromatin domainsBarrier insulatorsChIP-seqRegulatory signaturesMammalian cellsGene promoterEnhancer-blocking insulator activityTissue-specific gene expressionUpstream stimulatory factor proteinsHeterochromatin-mediated geneGenome-wide occupancyRole of CTCFRecruitment of enzymesΒ-globin locusHistone methyltransferase activityHuman primary erythroid cellsSpecific transcription factorsChicken β-globin locusProximal promoter regionProtein CTCFChromatin boundaries