1998
Reaction of nitric oxide with superoxide inhibits basolateral K+ channels in the rat CCD
Lu M, Wang W. Reaction of nitric oxide with superoxide inhibits basolateral K+ channels in the rat CCD. American Journal Of Physiology 1998, 275: c309-c316. PMID: 9688863, DOI: 10.1152/ajpcell.1998.275.1.c309.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell MembraneCyclic GMPDitiocarbIn Vitro TechniquesKidney CortexKidney Tubules, CollectingMembrane PotentialsNitratesNitric OxideNitroprussidePatch-Clamp TechniquesPenicillaminePotassium ChannelsPotassium Channels, Calcium-ActivatedPyrogallolRatsRats, Sprague-DawleyS-Nitroso-N-AcetylpenicillamineSmall-Conductance Calcium-Activated Potassium ChannelsSuperoxidesConceptsNitric oxideInhibitory effectChannel activityPatch-clamp techniqueCGMP-dependent pathwayMicroM SNAPRat CCDCGMP productionNO donorRat kidneyControl valuesMM TironIntracellular scavengerExogenous cGMPSuperoxide dismutaseOpen probabilityControl conditionHigh concentrationsDiethyldithiocarbamic acidDonorsKidney
1997
Nitric Oxide Links the Apical Na+ Transport to the Basolateral K+ Conductance in the Rat Cortical Collecting Duct
Lu M, Giebisch G, Wang W. Nitric Oxide Links the Apical Na+ Transport to the Basolateral K+ Conductance in the Rat Cortical Collecting Duct. The Journal Of General Physiology 1997, 110: 717-726. PMID: 9382898, PMCID: PMC2229403, DOI: 10.1085/jgp.110.6.717.Peer-Reviewed Original ResearchMeSH KeywordsAmilorideAnimalsAnti-Arrhythmia AgentsBiological TransportCalciumCyclic GMPDiureticsEnzyme InhibitorsKidney Tubules, CollectingNG-Nitroarginine Methyl EsterNitric OxideNitric Oxide SynthasePatch-Clamp TechniquesPenicillaminePotassiumPotassium ChannelsRatsRats, Sprague-DawleyS-Nitroso-N-AcetylpenicillamineSodiumSodium ChannelsSpecific Pathogen-Free OrganismsConceptsEffects of amilorideIntracellular Ca2Cell-attached patchesControl valuesChannel activityRat cortical collecting ductsNO-cGMP dependent pathwayNitric oxide synthaseFree bath solutionCortical collecting ductsNa/H exchangerPatch-clamp techniqueAddition of SNAPMicroM benzamilOxide synthaseChannel blockersFura-2MicroM amilorideExtracellular Ca2Rat CCDRemoval of Ca2Clamp techniqueNeuronal formCollecting ductsRat kidneyNitric oxide-induced hyperpolarization stimulates low-conductance Na+ channel of rat CCD
Lu M, Giebisch G, Wang W. Nitric oxide-induced hyperpolarization stimulates low-conductance Na+ channel of rat CCD. American Journal Of Physiology 1997, 272: f498-f504. PMID: 9140051, DOI: 10.1152/ajprenal.1997.272.4.f498.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCyclic GMPElectric ConductivityKidney CortexKidney Tubules, CollectingKineticsMembrane PotentialsModels, BiologicalNG-Nitroarginine Methyl EsterNitric OxideNitric Oxide SynthasePatch-Clamp TechniquesPenicillamineRatsRats, Sprague-DawleyS-Nitroso-N-AcetylpenicillamineSodium ChannelsTime FactorsConceptsL-NAMESodium nitroprussideNitric oxideNitro-L-arginine methyl esterL-NAME-induced decreaseAction of NOL-NAME resultsS-nitroso-N-acetyl penicillamineEffect of NOEffect of SNAPWhole-cell recordingsMicroM sodium nitroprussidePatch-clamp techniqueExogenous NO donorD-NAMENO synthaseMicroM amilorideRat CCDCell recordingsNO donorControl valuesStimulatory effectIndirect actionDependent pathwayHyperpolarization
1996
Effect of angiotensin II on the apical K+ channel in the thick ascending limb of the rat kidney.
Lu M, Zhu Y, Balazy M, Reddy K, Falck J, Wang W. Effect of angiotensin II on the apical K+ channel in the thick ascending limb of the rat kidney. The Journal Of General Physiology 1996, 108: 537-547. PMID: 8972391, PMCID: PMC2229337, DOI: 10.1085/jgp.108.6.537.Peer-Reviewed Original ResearchMeSH KeywordsAngiotensin IIAnimalsCell MembraneCytochrome P-450 Enzyme InhibitorsDose-Response Relationship, DrugEnzyme InhibitorsFatty Acids, UnsaturatedHydroxyeicosatetraenoic AcidsIn Vitro TechniquesLoop of HenleNaphthalenesNG-Nitroarginine Methyl EsterPatch-Clamp TechniquesPenicillaminePotassium Channel BlockersPotassium ChannelsRatsRats, Sprague-DawleyS-Nitroso-N-AcetylpenicillamineSodiumConceptsEffect of AIIThick ascending limbAngiotensin IIControl valuesInhibitory effectChannel activityAscending limbRat kidneyL-arginine methyl esterArachidonic acidStimulatory effectExogenous nitric oxide donorNM angiotensin IIMicroM NG-nitroNitric oxide synthaseMajor AA metaboliteNitric oxide donorMicroM calphostin CPatch-clamp techniqueP450-dependent pathwayL-NAMENG-nitroCell-attached patchesOxide synthaseP450 metabolitesCytochrome P-450 metabolites mediate extracellular Ca(2+)-induced inhibition of apical K+ channels in the TAL
Wang W, Lu M, Hebert S. Cytochrome P-450 metabolites mediate extracellular Ca(2+)-induced inhibition of apical K+ channels in the TAL. American Journal Of Physiology 1996, 271: c103-c111. PMID: 8760035, DOI: 10.1152/ajpcell.1996.271.1.c103.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAnimalsCalciumCell MembraneCytochrome P-450 Enzyme SystemExtracellular SpaceFatty Acids, UnsaturatedIn Vitro TechniquesLoop of HenleNeomycinPatch-Clamp TechniquesPotassium Channel BlockersPotassium ChannelsProtein Kinase CRatsRats, Sprague-DawleyTetradecanoylphorbol AcetateConceptsThick ascending limbCell-attached patchesChannel activityProtein kinase CCytochrome PArachidonic acidControl valuesInhibitory effectPatch-clamp techniqueEffects of neomycinCalphostin CMicroM neomycinExtracellular Ca2Ascending limbAddition of phorbolRat kidneyChannel open probabilityTAL tubulesNM calphostin CInhibition of PKC