2013
Sustained delivery of proangiogenic microRNA‐132 by nanoparticle transfection improves endothelial cell transplantation
Devalliere J, Chang WG, Andrejecsk JW, Abrahimi P, Cheng CJ, Jane‐wit D, Saltzman WM, Pober JS. Sustained delivery of proangiogenic microRNA‐132 by nanoparticle transfection improves endothelial cell transplantation. The FASEB Journal 2013, 28: 908-922. PMID: 24221087, PMCID: PMC3898640, DOI: 10.1096/fj.13-238527.Peer-Reviewed Original ResearchConceptsHuman umbilical vein ECsEndothelial cellsMiR-132MicroRNA-132Cultured human umbilical vein endothelial cellsNumber of microvesselsGrowth factor-induced proliferationHuman umbilical vein endothelial cellsUmbilical vein endothelial cellsEndothelial cell transplantationCultured endothelial cellsEndogenous growth factorsEC transplantationVein endothelial cellsCell transplantationImmunodeficient miceTissue perfusionTransplantationMiR deliveryGrowth factorIntegrin αvβ3Endocytosed nanoparticlesSquare millimeterBiological effectsControl transfection
2004
CULTURED HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS IMPLANTED IN IMMUNODEFICIENT MICE CAN ACQUIRE ARTERIOLAR, CAPILLARY, AND VENULAR FEATURES
Enis D, Wang Y, Kashgarian M, Schechner J, Pober J. CULTURED HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS IMPLANTED IN IMMUNODEFICIENT MICE CAN ACQUIRE ARTERIOLAR, CAPILLARY, AND VENULAR FEATURES. Cardiovascular Pathology 2004, 13: 19-20. DOI: 10.1016/j.carpath.2004.03.052.Peer-Reviewed Original ResearchCultured human umbilical vein endothelial cellsHuman umbilical vein endothelial cellsUmbilical vein endothelial cellsVein endothelial cellsEndothelial cellsArteriolar
2000
Caveolin-1 Associates with TRAF2 to Form a Complex That Is Recruited to Tumor Necrosis Factor Receptors*
Feng X, Gaeta M, Madge L, Yang J, Bradley J, Pober J. Caveolin-1 Associates with TRAF2 to Form a Complex That Is Recruited to Tumor Necrosis Factor Receptors*. Journal Of Biological Chemistry 2000, 276: 8341-8349. PMID: 11112773, DOI: 10.1074/jbc.m007116200.Peer-Reviewed Original ResearchConceptsCaveolin-1Confocal fluorescence microscopyIntracellular regionNecrosis factor receptor-associated factor 2TNF receptor 2Receptor-associated factor 2TNF receptor 1Promoter-reporter geneCaveolin-1 associatesFluorescence microscopyProtein caveolin-1Caveolin-1 proteinHuman embryonic kidney 293 cellsIntracellular adapter proteinEmbryonic kidney 293 cellsAbsence of ligandRegions of enrichmentKidney 293 cellsEndogenous TRAF2HEK-293 cellsAdapter proteinCultured human umbilical vein endothelial cellsHuman umbilical vein endothelial cellsPlasma membraneUmbilical vein endothelial cells
1996
Ceramide Is Not a Signal for Tumor Necrosis Factor–Induced Gene Expression but Does Cause Programmed Cell Death in Human Vascular Endothelial Cells
Slowik M, De Luca L, Min W, Pober J. Ceramide Is Not a Signal for Tumor Necrosis Factor–Induced Gene Expression but Does Cause Programmed Cell Death in Human Vascular Endothelial Cells. Circulation Research 1996, 79: 736-747. PMID: 8831497, DOI: 10.1161/01.res.79.4.736.Peer-Reviewed Original ResearchConceptsConcentrations of TNFNF-kappa BEndothelial cellsJun N-terminal kinaseNecrosis factorEndothelial leukocyte adhesion molecule-1Leukocyte adhesion molecule-1Treatment of ECCell deathCultured human umbilical vein endothelial cellsEndothelial leukocyte adhesion moleculeTumor necrosis factorAdhesion molecule-1Leukocyte adhesion moleculesHuman vascular endothelial cellsHuman umbilical vein endothelial cellsStimulation of ECUmbilical vein endothelial cellsVascular endothelial cellsP50/p65 heterodimeric formVein endothelial cellsPotential intracellular mediators
1995
CD40 on human endothelial cells: inducibility by cytokines and functional regulation of adhesion molecule expression.
Karmann K, Hughes CC, Schechner J, Fanslow WC, Pober JS. CD40 on human endothelial cells: inducibility by cytokines and functional regulation of adhesion molecule expression. Proceedings Of The National Academy Of Sciences Of The United States Of America 1995, 92: 4342-4346. PMID: 7538666, PMCID: PMC41940, DOI: 10.1073/pnas.92.10.4342.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodies, MonoclonalAntigens, CDAntigens, Differentiation, B-LymphocyteCD40 AntigensCell Adhesion MoleculesCells, CulturedCytokinesEndothelium, VascularGene ExpressionGranulocyte-Macrophage Colony-Stimulating FactorHumansInterferon-betaInterferon-gammaInterleukinsKineticsMiceRecombinant ProteinsSkinTumor Necrosis Factor-alphaUmbilical VeinsConceptsTumor necrosis factorAdhesion molecule-1Endothelial cellsHuman endothelial cellsNecrosis factorInterleukin-1Molecule-1T cell-mediated inflammatory reactionVascular cell adhesion molecule-1Intercellular adhesion molecule-1Cell adhesion molecule-1Cultured human umbilical vein endothelial cellsAdhesion molecule expressionLeukocyte adhesion moleculesMicrovascular endothelial cellsHuman microvascular endothelial cellsHuman umbilical vein endothelial cellsUmbilical vein endothelial cellsFlow cytometric quantitationVein endothelial cellsCD40 antigenEndothelial CD40Interferon betaMolecule expressionInflammatory reactionIL-8 and angiogenesis: evidence that human endothelial cells lack receptors and do not respond to IL-8 in vitro
Petzelbauer P, Watson C, Watson C, Pfau S, Pober J. IL-8 and angiogenesis: evidence that human endothelial cells lack receptors and do not respond to IL-8 in vitro. Cytokine 1995, 7: 267-272. PMID: 7543779, DOI: 10.1006/cyto.1995.0031.Peer-Reviewed Original ResearchConceptsHuman umbilical vein endothelial cellsIL-8Cultured human umbilical vein endothelial cellsDermal microvascular endothelial cellsEndothelial cellsHuman endothelial cellsRecombinant human IL-8IL-8 treatmentMicrovascular human endothelial cellsReceptor mRNA expressionEndothelial cell growth factorHuman IL-8Human dermal microvascular endothelial cellsMicrovascular endothelial cellsCytoplasmic calcium concentrationUmbilical vein endothelial cellsCell growth factorVein endothelial cellsAngiogenic factorsMRNA expressionDirect actionGrowth factorCalcium concentrationIndirect actionProliferative rateDisparate localization of 55-kd and 75-kd tumor necrosis factor receptors in human endothelial cells.
Bradley JR, Thiru S, Pober JS. Disparate localization of 55-kd and 75-kd tumor necrosis factor receptors in human endothelial cells. American Journal Of Pathology 1995, 146: 27-32. PMID: 7856733, PMCID: PMC1870772.Peer-Reviewed Original ResearchConceptsTumor necrosis factor receptorNecrosis factor receptorCell surfaceFactor receptorConfocal immunofluorescence microscopyDisparate localizationUndergoes endocytosisCultured human umbilical vein endothelial cellsHuman umbilical vein endothelial cellsEndothelial cellsUmbilical vein endothelial cellsReceptor clusteringGolgi apparatusHuman endothelial cellsCoated vesiclesVein endothelial cellsImmunofluorescence microscopyCellular distributionTNF receptorEndothelial cell activationCell activationReceptorsCytoplasmic vacuolesCellsEndocytosis
1993
Elevated cyclic AMP inhibits endothelial cell synthesis and expression of TNF-induced endothelial leukocyte adhesion molecule-1, and vascular cell adhesion molecule-1, but not intercellular adhesion molecule-1.
Pober JS, Slowik MR, De Luca LG, Ritchie AJ. Elevated cyclic AMP inhibits endothelial cell synthesis and expression of TNF-induced endothelial leukocyte adhesion molecule-1, and vascular cell adhesion molecule-1, but not intercellular adhesion molecule-1. The Journal Of Immunology 1993, 150: 5114-23. PMID: 7684420, DOI: 10.4049/jimmunol.150.11.5114.Peer-Reviewed Original ResearchConceptsEndothelial cellsAction of cAMPCultured human umbilical vein endothelial cellsHuman umbilical vein endothelial cellsSignal transducerUmbilical vein endothelial cellsRole of cAMPCell adhesion molecule-1Adhesion molecule-1Vein endothelial cellsCyclic monophosphatePhysiological regulatorElevated cyclic AMPVascular cell adhesion molecule-1Adhesion molecule expressionIntracellular mediatorsIsobutyl methylxanthineMolecule-1TNF inductionVCAM-1 mRNA
1988
LYTIC ANTI-ENDOTHELIAL CELL ANTIBODIES IN HAEMOLYTIC-URAEMIC SYNDROME
Leung D, Havens P, Moake J, Kim M, Pober J. LYTIC ANTI-ENDOTHELIAL CELL ANTIBODIES IN HAEMOLYTIC-URAEMIC SYNDROME. The Lancet 1988, 332: 183-186. PMID: 2899661, DOI: 10.1016/s0140-6736(88)92287-8.Peer-Reviewed Original ResearchConceptsAnti-endothelial cell antibodiesHaemolytic uraemic syndromeThrombotic thrombocytopenic purpuraCell antibodiesComplement-fixing IgGDisorder of immunoregulationAnti-endothelial antibodiesEndothelial cell antigensCultured human umbilical vein endothelial cellsGamma-interferon treatmentHuman umbilical vein endothelial cellsUmbilical vein endothelial cellsAdult patientsVein endothelial cellsThrombocytopenic purpuraIgM antibodiesVascular injuryInterferon treatmentUraemic syndromeGamma interferonCell antigensControl seraEndothelial cellsAntibodiesSerum
1987
Induction of an activation antigen on postcapillary venular endothelium in human skin organ culture.
Messadi DV, Pober JS, Fiers W, Gimbrone MA, Murphy GF. Induction of an activation antigen on postcapillary venular endothelium in human skin organ culture. The Journal Of Immunology 1987, 139: 1557-62. PMID: 3497975, DOI: 10.4049/jimmunol.139.5.1557.Peer-Reviewed Original ResearchConceptsTumor necrosis factorActivation antigensNecrosis factorInterleukin-1Microvascular endotheliumEndothelial cellsEndothelial activation antigenMediators interleukin-1Postcapillary venular endotheliumCultured human umbilical vein endothelial cellsSuperficial vascular plexusOrgan cultureHuman skin organ cultureShort-term organ cultureHuman umbilical vein endothelial cellsSkin organ cultureUmbilical vein endothelial cellsVenular endothelial cellsMicrovascular stainingPostcapillary venular endothelial cellsVein endothelial cellsEndogenous cytokinesLymphocyte traffickingInterleukin-2Venular endotheliumComposition of the von Willebrand factor storage organelle (Weibel-Palade body) isolated from cultured human umbilical vein endothelial cells.
Ewenstein BM, Warhol MJ, Handin RI, Pober JS. Composition of the von Willebrand factor storage organelle (Weibel-Palade body) isolated from cultured human umbilical vein endothelial cells. Journal Of Cell Biology 1987, 104: 1423-1433. PMID: 3494734, PMCID: PMC2114468, DOI: 10.1083/jcb.104.5.1423.Peer-Reviewed Original ResearchConceptsWeibel-Palade bodiesSecretory organellesUmbilical vein endothelial cellsOrganelle fractionsVein endothelial cellsCultured endothelial cellsIntracellular protein traffickingVon Willebrand factorVon Willebrand factor storageEndothelial cellsSelf-generating Percoll gradientsDirect morphological examinationCultured umbilical vein endothelial cellsUnmodified formProtein traffickingCultured human umbilical vein endothelial cellsRegulated secretionStimulation of ECHuman umbilical vein endothelial cellsStorage organellesOrganellesDense organellesPro sequenceProteolytic processingAdhesive glycoproteinCultured human endothelial cells express platelet-derived growth factor A chain.
Collins T, Pober JS, Gimbrone MA, Hammacher A, Betsholtz C, Westermark B, Heldin CH. Cultured human endothelial cells express platelet-derived growth factor A chain. American Journal Of Pathology 1987, 126: 7-12. PMID: 2433948, PMCID: PMC1899550.Peer-Reviewed Original ResearchConceptsRNA speciesSame relative abundanceHuman endothelial cellsRelative abundanceB polypeptide chainsEndothelial cellsChain transcriptsC-sis geneB-chain transcriptsPlatelet-derived growth factorExtensive structural dataTranscript speciesCultured human umbilical vein endothelial cellsB-chain expressionBovine aortic endothelial cellsHuman umbilical vein endothelial cellsDimeric proteinUmbilical vein endothelial cellsCultured vascular endothelial cellsDifferent genesPrincipal cell typesHybridization analysisPolypeptide chainVein endothelial cellsCell types
1983
Ia expression by vascular endothelium is inducible by activated T cells and by human gamma interferon.
Pober JS, Gimbrone MA, Cotran RS, Reiss CS, Burakoff SJ, Fiers W, Ault KA. Ia expression by vascular endothelium is inducible by activated T cells and by human gamma interferon. Journal Of Experimental Medicine 1983, 157: 1339-1353. PMID: 6403654, PMCID: PMC2186979, DOI: 10.1084/jem.157.4.1339.Peer-Reviewed Original ResearchConceptsAllogeneic T cellsT cellsIa antigensEndothelial cellsPeripheral blood mononuclear cellsBlood mononuclear cellsCultured human umbilical vein endothelial cellsExpression of IaActivated T cellsHuman umbilical vein endothelial cellsUmbilical vein endothelial cellsAllograft rejectionVein endothelial cellsGamma interferon geneFluorescence flow cytometryHuman gamma interferonMononuclear cellsImmune responseGamma interferonVascular endotheliumIa expressionFlow cytometryImmune interferonMonoclonal antibodiesAntigen