1992
Karyoplasmic interaction selection strategy: a general strategy to detect protein-protein interactions in mammalian cells.
Fearon E, Finkel T, Gillison M, Kennedy S, Casella J, Tomaselli G, Morrow J, Van Dang C. Karyoplasmic interaction selection strategy: a general strategy to detect protein-protein interactions in mammalian cells. Proceedings Of The National Academy Of Sciences Of The United States Of America 1992, 89: 7958-7962. PMID: 1387709, PMCID: PMC49834, DOI: 10.1073/pnas.89.17.7958.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsCHO CellsCricetinaeCytoplasmDNA-Binding ProteinsFlow CytometryFungal ProteinsHerpes Simplex Virus Protein Vmw65Hybrid CellsMacromolecular SubstancesMolecular Sequence DataProtein BindingRecombinant ProteinsSaccharomyces cerevisiae ProteinsTranscription FactorsTransfectionViral ProteinsConceptsProtein-protein interactionsMammalian cellsChimeric proteinReporter geneYeast transcriptional activator GAL4KISS systemSpecific protein-protein interactionsTranscriptional activator GAL4Transcriptional activation domainNovel protein interactionsDNA-binding domainTranscriptional activation functionSpecific interactionsActivator GAL4Activation domainProtein interactionsResultant transcriptionCDNA librarySelectable markerGAL4Drug resistance markersCell surfaceProteinTranscriptionGenes
1988
Comparison of nonerythroid alpha-spectrin genes reveals strict homology among diverse species.
Leto T, Fortugno-Erikson D, Barton D, Yang-Feng T, Francke U, Harris A, Morrow J, Marchesi V, Benz E. Comparison of nonerythroid alpha-spectrin genes reveals strict homology among diverse species. Molecular And Cellular Biology 1988, 8: 1-9. PMID: 3336352, PMCID: PMC363070, DOI: 10.1128/mcb.8.1.1.Peer-Reviewed Original ResearchConceptsAlpha-spectrin geneErythroid alpha-spectrin geneHuman chromosome 9Alpha-spectrin chainComparison of sequencesCarboxy-terminal sequenceErythroid genesDiverse speciesExpression libraryChromosome 1Hydrophobic residuesErythroid spectrinAlpha-spectrinFilamentous proteinsFunctional sitesGenesChromosome 9Human cloneStrict homologyRepeat patternCell membraneInvariant tryptophanSpectrinPeptide sequencesCDNAComparison of Nonerythroid α-Spectrin Genes Reveals Strict Homology among Diverse Species
Leto T, Fortugno-Erikson D, Barton D, Yang-Feng T, Francke U, Harris A, Morrow J, Marchesi V, Benz E. Comparison of Nonerythroid α-Spectrin Genes Reveals Strict Homology among Diverse Species. Molecular And Cellular Biology 1988, 8: 1-9. DOI: 10.1128/mcb.8.1.1-9.1988.Peer-Reviewed Original ResearchΑ-spectrin geneHuman chromosome 9Comparison of sequencesCarboxy-terminal sequenceErythroid genesDiverse speciesSpectrin geneExpression libraryChromosome 1Hydrophobic residuesErythroid spectrinFilamentous proteinsFunctional sitesGenesChromosome 9Human cloneStrict homologyΑ-spectrinRepeat patternCell membraneInvariant tryptophanSpectrinPeptide sequencesCDNASpecies