2000
Rat sponge implant model: a new system for evaluating angiogenic gene transfer.
Wang H, Gordon D, Olszewski B, Song Y, Kovesdi I, Keiser J. Rat sponge implant model: a new system for evaluating angiogenic gene transfer. International Journal Of Molecular Medicine 2000, 6: 645-53. PMID: 11078823, DOI: 10.3892/ijmm.6.6.645.Peer-Reviewed Original ResearchMeSH KeywordsAdenoviridaeAnimalsBeta-GalactosidaseBlood VesselsDNA, RecombinantDose-Response Relationship, DrugEndothelial Growth FactorsGene Transfer TechniquesGene Transfer, HorizontalHumansImmunohistochemistryLymphokinesModels, CardiovascularNeovascularization, PathologicProstheses and ImplantsRatsRecombinant Fusion ProteinsSurgical SpongesTime FactorsVascular Endothelial Growth Factor AVascular Endothelial Growth FactorsVon Willebrand FactorConceptsBeta-galactosidase reporter geneSuch genesReporter geneGenesGene transferTransfection efficiencyAngiogenic genesGene therapyProtein levelsProtein expressionVEGF121 geneReplication-deficient adenovirus vectorModel systemSponge implant modelAngiogenic responseProtein injectionAngiogenesisAdenovirus vectorVEGF protein expressionAngiogenic effectsProtein concentrationEnhanced angiogenesisAngiogenic efficacyGene transfection efficiencyHigh levels
1996
Synthesis of tissue inhibitor of metalloproteinase-1 (TIMP-1) in rabbit aortic neointima after selective de-endothelialization
Wang H, Moore S, Alavi M. Synthesis of tissue inhibitor of metalloproteinase-1 (TIMP-1) in rabbit aortic neointima after selective de-endothelialization. Atherosclerosis 1996, 126: 95-104. PMID: 8879438, DOI: 10.1016/0021-9150(96)05898-4.Peer-Reviewed Original ResearchConceptsTIMP-1 geneRabbit formsApparent homologyExtracellular matrix metabolismExpression systemTIMP-1 expressionSoluble proteinNorthern blotSitu hybridization techniqueEscherichia coliProtein levelsMatrix metabolismGenesAortic neointimaNeointimal SMCsHybridization techniqueNucleic acidsWestern blotTIMP-1Tissue inhibitorExpressionBlotMetalloproteinase-1CDNAInhibitors