In-situ high-resolution 3D imaging combined with proteomics and metabolomics reveals enlargement of subcellular architecture and enhancement of photosynthesis pathways in nuclear-irradiated Chlorella pyrenoidosa
Guo W, Feng L, Wang Z, Guo J, Park D, Carroll B, Zhang X, Liu J, Cheng J. In-situ high-resolution 3D imaging combined with proteomics and metabolomics reveals enlargement of subcellular architecture and enhancement of photosynthesis pathways in nuclear-irradiated Chlorella pyrenoidosa. Chemical Engineering Journal 2022, 430: 133037. DOI: 10.1016/j.cej.2021.133037.Peer-Reviewed Original ResearchPhotosynthetic efficiencySubcellular organellesChlorella pyrenoidosaHigh growth rateCryo-electron tomographyWild-type cellsPhotosynthesis pathwaySubcellular architectureCarbon fixationCryo-FIB millingChlorophyll synthesisMutantsGrowth rateMacroscopic traitsOrganellesSubcellular morphologyC. pyrenoidosaProteomicsBeam scanning electron microscopyIon beam scanning electron microscopyMetabolomics dataPyrenoidosaUnprecedented detailSuch modificationsChloroplasts