2024
CRISPR-Cas9-based approaches for genetic analysis and epistatic interaction studies in Coxiella burnetii.
Steiner S, Roy C. CRISPR-Cas9-based approaches for genetic analysis and epistatic interaction studies in Coxiella burnetii. MSphere 2024, e0052324. PMID: 39560384, DOI: 10.1128/msphere.00523-24.Peer-Reviewed Original ResearchIntracellular replicationHost cellsDouble mutantGenetic analysisDot/Icm type IVB secretion systemType IVB secretion systemConstruction of double mutantsLoss-of-function mutantsIntracellular replication defectsLysosome-derived vacuoleIVB secretion systemObligate intracellular bacterial pathogenRandom transposon mutagenesisTransposon insertion mutantsCRISPR-Cas9-based technologiesIntracellular bacterial pathogensLoss-of-function mutationsMammalian host cellsIntroduction of premature stop codonsPremature stop codonSilence gene expressionTargeted gene disruptionBase editing approachesEffector mutantsSecretion system
2022
Developmental Transitions Coordinate Assembly of the Coxiella burnetii Dot/Icm Type IV Secretion System
Park D, Steiner S, Shao M, Roy CR, Liu J. Developmental Transitions Coordinate Assembly of the Coxiella burnetii Dot/Icm Type IV Secretion System. Infection And Immunity 2022, 90: e00410-22. PMID: 36190257, PMCID: PMC9584302, DOI: 10.1128/iai.00410-22.Peer-Reviewed Original ResearchConceptsSmall cell variantLarge cell variantDot/Icm type IV secretion systemCell variantIntracellular replicationActive large cell variantHost cellsIntracellular bacterial pathogenType IV secretion systemDot/Icm T4SSObligate intracellular bacterial pathogenC. burnetiiCoxiella burnetiiBiphasic developmental cycleUnique biphasic developmental cycleInfectionNew host cellsSecretion systemBacterial pathogensInfectious formHost vacuoleBurnetiiMorphological changesCellsLater stages
2021
Coxiella burnetii encodes an LvgA‐related protein important for intracellular replication
Steiner S, Meir A, Roy CR. Coxiella burnetii encodes an LvgA‐related protein important for intracellular replication. Cellular Microbiology 2021, 23: e13331. PMID: 33774901, DOI: 10.1111/cmi.13331.Peer-Reviewed Original ResearchConceptsIntracellular replicationDot/Icm type IVB secretion systemType IVB secretion systemDot/Icm apparatusHost cell cytosolTransposon mutagenesis screenAutophagy protein LC3Lysosome-derived organellesL. pneumophilaSecondary structure predictionChaperone complexBiogenesis defectsEffector proteinsEffector translocationProtein complexesMutagenesis screenSecretion systemLvgACell cytosolNine Mile phase IIProtein LC3MutantsReplication phenotypeProteinStructure prediction