2019
Transfection of Mammalian Cells with Calcium Phosphate-DNA Coprecipitates.
Kumar P, Nagarajan A, Uchil PD. Transfection of Mammalian Cells with Calcium Phosphate-DNA Coprecipitates. Cold Spring Harbor Protocols 2019, 2019: pdb.top096255. PMID: 31575800, DOI: 10.1101/pdb.top096255.Peer-Reviewed Original ResearchCalcium Phosphate-Mediated Transfection of Eukaryotic Cells with Plasmid DNAs.
Kumar P, Nagarajan A, Uchil PD. Calcium Phosphate-Mediated Transfection of Eukaryotic Cells with Plasmid DNAs. Cold Spring Harbor Protocols 2019, 2019: pdb.prot095430. PMID: 31575793, DOI: 10.1101/pdb.prot095430.Peer-Reviewed Original ResearchCalcium Phosphate-Mediated Transfection of Cells with High-Molecular-Weight Genomic DNA.
Kumar P, Nagarajan A, Uchil PD. Calcium Phosphate-Mediated Transfection of Cells with High-Molecular-Weight Genomic DNA. Cold Spring Harbor Protocols 2019, 2019: pdb.prot095448. PMID: 31575794, DOI: 10.1101/pdb.prot095448.Peer-Reviewed Original ResearchCalcium Phosphate-Mediated Transfection of Adherent Cells or Cells Growing in Suspension: Variations on the Basic Method
Kumar P, Nagarajan A, Uchil PD. Calcium Phosphate-Mediated Transfection of Adherent Cells or Cells Growing in Suspension: Variations on the Basic Method. Cold Spring Harbor Protocols 2019, 2019: pdb.prot095455. PMID: 31575795, DOI: 10.1101/pdb.prot095455.Peer-Reviewed Original ResearchIntroducing Genes into Cultured Mammalian Cells
Kumar P, Nagarajan A, Uchil PD. Introducing Genes into Cultured Mammalian Cells. Cold Spring Harbor Protocols 2019, 2019: pdb.top095406. PMID: 31285274, DOI: 10.1101/pdb.top095406.Peer-Reviewed Original ResearchDNA Transfection by Electroporation.
Kumar P, Nagarajan A, Uchil PD. DNA Transfection by Electroporation. Cold Spring Harbor Protocols 2019, 2019: pdb.prot095471. PMID: 31262956, DOI: 10.1101/pdb.prot095471.Peer-Reviewed Original ResearchDNA Transfection Mediated by Cationic Lipid Reagents
Kumar P, Nagarajan A, Uchil PD. DNA Transfection Mediated by Cationic Lipid Reagents. Cold Spring Harbor Protocols 2019, 2019: pdb.prot095414. PMID: 30824617, DOI: 10.1101/pdb.prot095414.Peer-Reviewed Original ResearchConceptsCell typesSpecific cell typesCationic lipid reagentsSuspension cell typesDNA transfectionLiposomal transfection reagentTransfection procedureCell linesLipid reagentsFuGENE 6Transfection reagentPresence of serumLipofectamine 2000Number of manipulationsGeneralistsTransfectionAlternative protocolManipulationCellsMost adherentLipofectinLipofection.
Kumar P, Nagarajan A, Uchil PD. Lipofection. Cold Spring Harbor Protocols 2019, 2019: pdb.top096248. PMID: 30824627, DOI: 10.1101/pdb.top096248.Peer-Reviewed Original Research
2018
Optical Transfection.
Kumar P, Nagarajan A, Uchil PD. Optical Transfection. Cold Spring Harbor Protocols 2018, 2018: pdb.top096222. PMID: 30510131, DOI: 10.1101/pdb.top096222.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell MembraneEukaryotic CellsHumansMammalsOptical DevicesOptical PhenomenaTransfectionSelective Agents for Stable Transfection.
Kumar P, Nagarajan A, Uchil PD. Selective Agents for Stable Transfection. Cold Spring Harbor Protocols 2018, 2018: pdb.top096230. PMID: 30181228, DOI: 10.1101/pdb.top096230.Peer-Reviewed Original ResearchTransfection Mediated by DEAE-Dextran.
Kumar P, Nagarajan A, Uchil PD. Transfection Mediated by DEAE-Dextran. Cold Spring Harbor Protocols 2018, 2018: pdb.prot095463. PMID: 29967275, DOI: 10.1101/pdb.prot095463.Peer-Reviewed Original ResearchDEAE-Dextran Transfection.
Kumar P, Nagarajan A, Uchil PD. DEAE-Dextran Transfection. Cold Spring Harbor Protocols 2018, 2018: pdb.top096263. PMID: 29967279, DOI: 10.1101/pdb.top096263.Peer-Reviewed Original Research
2014
Attachment of Cell-Binding Ligands to Arginine-Rich Cell-Penetrating Peptides Enables Cytosolic Translocation of Complexed siRNA
Zeller S, Choi CS, Uchil PD, Ban HS, Siefert A, Fahmy TM, Mothes W, Lee SK, Kumar P. Attachment of Cell-Binding Ligands to Arginine-Rich Cell-Penetrating Peptides Enables Cytosolic Translocation of Complexed siRNA. Cell Chemical Biology 2014, 22: 50-62. PMID: 25544044, PMCID: PMC4320807, DOI: 10.1016/j.chembiol.2014.11.009.Peer-Reviewed Original Research