2022
Protocol for assessing translational regulation in mammalian cell lines by OP-Puro labeling
Hsu JC, Pawlak JB, Laurent-Rolle M, Cresswell P. Protocol for assessing translational regulation in mammalian cell lines by OP-Puro labeling. STAR Protocols 2022, 3: 101654. PMID: 36072758, PMCID: PMC9442383, DOI: 10.1016/j.xpro.2022.101654.Peer-Reviewed Original ResearchConceptsTranslational regulationMammalian cell linesDifferent cell typesDifferent growth conditionsMammalian cellsTranslation activityGene expressionBiological processesCell typesComplete detailsConfocal microscopyCell linesGrowth conditionsPuromycin analoguesCritical roleRegulationCellsProteinLabelingFundamental stepExpressionClick chemistry reactionActivityCytometry
2010
The NS5 Protein of the Virulent West Nile Virus NY99 Strain Is a Potent Antagonist of Type I Interferon-Mediated JAK-STAT Signaling
Laurent-Rolle M, Boer EF, Lubick KJ, Wolfinbarger JB, Carmody AB, Rockx B, Liu W, Ashour J, Shupert WL, Holbrook MR, Barrett AD, Mason PW, Bloom ME, García-Sastre A, Khromykh AA, Best SM. The NS5 Protein of the Virulent West Nile Virus NY99 Strain Is a Potent Antagonist of Type I Interferon-Mediated JAK-STAT Signaling. Journal Of Virology 2010, 84: 3503-3515. PMID: 20106931, PMCID: PMC2838099, DOI: 10.1128/jvi.01161-09.Peer-Reviewed Original ResearchConceptsPY-STAT1IFN antagonismJAK-STAT signal transductionIFN antagonistAlpha/beta interferonSignal transductionWild-type virusAnalogous residuesIFN-dependent gene expressionGene expressionJAK-STATKunjin virusSingle residueHost innate responseNonstructural protein NS5NS5 proteinSTAT1 phosphorylationSTAT2 degradationPoor suppressorNY99 strainPresence of IFNMutationsIFN-alpha/NS5Beta interferon
2003
Inhibition of interferon signaling by dengue virus
Muñoz-Jordán J, Sánchez-Burgos GG, Laurent-Rolle M, García-Sastre A. Inhibition of interferon signaling by dengue virus. Proceedings Of The National Academy Of Sciences Of The United States Of America 2003, 100: 14333-14338. PMID: 14612562, PMCID: PMC283592, DOI: 10.1073/pnas.2335168100.Peer-Reviewed Original ResearchConceptsPositive-strand RNA genomeExpression of NS4BPositive-strand RNA virusesNuclear signal transducerVirus-encoded proteinsStrand RNA virusesStrand RNA genomeHuman A549 cellsDengue virusRNA genomeDengue virus-infected cellsSignal transducerTranscription 1Gene expressionUnknown functionVirus-infected cellsInhibition of interferonRNA virusesDengue virus infectionNS4BMosquito-borne flavivirusProteinA549 cellsIFN responseViral products