2020
Mechanisms of substrate recognition by a typhoid toxin secretion-associated muramidase
Geiger T, Lara-Tejero M, Xiong Y, Galán JE. Mechanisms of substrate recognition by a typhoid toxin secretion-associated muramidase. ELife 2020, 9: e53473. PMID: 31958059, PMCID: PMC6996933, DOI: 10.7554/elife.53473.Peer-Reviewed Original Research
2018
Visualization of the type III secretion mediated Salmonella–host cell interface using cryo-electron tomography
Park D, Lara-Tejero M, Waxham MN, Li W, Hu B, Galán JE, Liu J. Visualization of the type III secretion mediated Salmonella–host cell interface using cryo-electron tomography. ELife 2018, 7: e39514. PMID: 30281019, PMCID: PMC6175578, DOI: 10.7554/elife.39514.Peer-Reviewed Original ResearchConceptsCryo-electron tomographyEffector translocationBacterial-host cell contactType III protein secretion systemProtein secretion systemHost cell interfaceProtein secretion machinesCell membraneComplex host-pathogen interactionsType III secretionHost-pathogen interactionsHost cell membraneTarget cell membraneNegative bacterial pathogensTranslocon poreSecretion machineEffector proteinsSecretion systemHost cellsBacterial pathogensCell contactCell interfaceIntimate associationTranslocationBacteria
2017
Metabolic and fitness determinants for in vitro growth and intestinal colonization of the bacterial pathogen Campylobacter jejuni
Gao B, Vorwerk H, Huber C, Lara-Tejero M, Mohr J, Goodman AL, Eisenreich W, Galán JE, Hofreuter D. Metabolic and fitness determinants for in vitro growth and intestinal colonization of the bacterial pathogen Campylobacter jejuni. PLOS Biology 2017, 15: e2001390. PMID: 28542173, PMCID: PMC5438104, DOI: 10.1371/journal.pbio.2001390.Peer-Reviewed Original ResearchMeSH KeywordsAbsorption, PhysiologicalAmino AcidsAnimalsAnti-Bacterial AgentsBacterial ProteinsCampylobacter InfectionsCampylobacter jejuniCation Transport ProteinsDNA Transposable ElementsDysbiosisGastroenteritisGene DeletionGenetic Association StudiesGenome, BacterialGenomic LibraryMice, Inbred C57BLMicrobial ViabilityModels, BiologicalMutagenesis, InsertionalMutationConceptsCampylobacter jejuniFood-producing animalsInfectious causesFood-borne illnessMost infectionsMouse modelHuman infectionsIntestinal colonizationIntestinal tractInfectionJejuni colonizationMouse intestineNext-generation sequencingIntracellular ion homeostasisC. jejuniFitness determinantsCertain nutrientsPathogenic strainsJejuniMetabolic requirementsComprehensive genome-wide analysisBacterial pathogen Campylobacter jejuniPathogen Campylobacter jejuniAnimalsGenome-wide analysis
2011
A Sorting Platform Determines the Order of Protein Secretion in Bacterial Type III Systems
Lara-Tejero M, Kato J, Wagner S, Liu X, Galán JE. A Sorting Platform Determines the Order of Protein Secretion in Bacterial Type III Systems. Science 2011, 331: 1188-1191. PMID: 21292939, PMCID: PMC3859126, DOI: 10.1126/science.1201476.Peer-Reviewed Original ResearchConceptsSecretion systemSorting platformType III protein secretion systemBacterial type III protein secretion systemsType III protein secretionProtein secretionType III secretion systemCytoplasmic sorting platformProtein secretion systemType III systemSalmonella enterica serovar TyphimuriumNovel antimicrobial strategiesEukaryotic cellsEffector proteinsEnterica serovar TyphimuriumCellular processesSerovar TyphimuriumImportant pathogenProteinAntimicrobial strategiesChaperonesSecretionPathogensMechanismSubstrate
2006
A MyD88-Deficient Mouse Model Reveals a Role for Nramp1 in Campylobacter jejuni Infection
Watson RO, Novik V, Hofreuter D, Lara-Tejero M, Galán JE. A MyD88-Deficient Mouse Model Reveals a Role for Nramp1 in Campylobacter jejuni Infection. Infection And Immunity 2006, 75: 1994-2003. PMID: 17194808, PMCID: PMC1865720, DOI: 10.1128/iai.01216-06.Peer-Reviewed Original ResearchConceptsJejuni infectionAdaptor protein myeloid differentiation factor 88Myeloid differentiation factor 88Most Toll-like receptorsMyD88-deficient miceDifferentiation factor 88Campylobacter jejuni infectionAdult immunocompetent miceToll-like receptorsMajor worldwide causeFactor 88Immunocompetent miceMouse modelMouse susceptibilityEnteric illnessWorldwide causeJejuni colonizationInfectionMiceC. jejuniCampylobacter jejuniPotential roleVirulence genesBacterial pathogensIsogenic derivativesAssembly of the inner rod determines needle length in the type III secretion injectisome
Marlovits TC, Kubori T, Lara-Tejero M, Thomas D, Unger VM, Galán JE. Assembly of the inner rod determines needle length in the type III secretion injectisome. Nature 2006, 441: 637-640. PMID: 16738660, DOI: 10.1038/nature04822.Peer-Reviewed Original Research