2023
Bulk and single-nucleus RNA sequencing highlight immune pathways induced in individuals during an Ixodes scapularis tick bite
Tang X, Lynn G, Cui Y, Cerny J, Arora G, Tomayko M, Craft J, Fikrig E. Bulk and single-nucleus RNA sequencing highlight immune pathways induced in individuals during an Ixodes scapularis tick bite. Infection And Immunity 2023, 91: e00282-23. PMID: 37846980, PMCID: PMC10652856, DOI: 10.1128/iai.00282-23.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceFeeding BehaviorGuinea PigsHumansIxodesRNA, Small NuclearTick BitesConceptsRNA sequencingSingle-nucleus RNA sequencingBulk RNA sequencingInterleukin-17 signalingPlatelet activation pathwaysLaboratory guinea pigsSnRNA-seqHippo signalingIndividual genesPeripheral bloodTick biteAdaptive immunityAnti-tick vaccinesGuidance pathwayImmune pathwaysNew biomarkersHost responseGuinea pigsHematophagous arthropodsHost defenseCell adhesionTick attachmentNovel insightsTick feedingPhysiological consequences
2018
Altered vector competence in an experimental mosquito-mouse transmission model of Zika infection
Uraki R, Hastings AK, Gloria-Soria A, Powell JR, Fikrig E. Altered vector competence in an experimental mosquito-mouse transmission model of Zika infection. PLOS Neglected Tropical Diseases 2018, 12: e0006350. PMID: 29505571, PMCID: PMC5854422, DOI: 10.1371/journal.pntd.0006350.Peer-Reviewed Original ResearchConceptsZIKV transmissionZika virus infectionNatural transmission routeInfected miceZIKV infectionZika infectionTesting vaccinesVirus infectionIntrathoracic injectionAnimal modelsZIKVInfectionMiceTransmission routesBlood mealVector competenceVector competencyMosquitoesTransmission modelVertebrate hostsMosquito modelPathogenesisVaccine
2013
Factor Xa Activation of Factor V Is of Paramount Importance in Initiating the Coagulation System
Schuijt TJ, Bakhtiari K, Daffre S, DePonte K, Wielders SJ, Marquart JA, Hovius JW, van der Poll T, Fikrig E, Bunce MW, Camire RM, Nicolaes GA, Meijers JC, van ‘t Veer C. Factor Xa Activation of Factor V Is of Paramount Importance in Initiating the Coagulation System. Circulation 2013, 128: 254-266. PMID: 23817575, PMCID: PMC3826089, DOI: 10.1161/circulationaha.113.003191.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnticoagulantsArthropod ProteinsBlood CoagulationBlood Coagulation TestsDose-Response Relationship, DrugFactor VFactor XaFactor Xa InhibitorsFeeding BehaviorFibrinogenHumansIxodesMutagenesisProtein Structure, TertiaryRabbitsRecombinant ProteinsSalivary Proteins and PeptidesSurface Plasmon ResonanceThrombin
2012
Enhanced Survival of Plasmodium-Infected Mosquitoes during Starvation
Zhao YO, Kurscheid S, Zhang Y, Liu L, Zhang L, Loeliger K, Fikrig E. Enhanced Survival of Plasmodium-Infected Mosquitoes during Starvation. PLOS ONE 2012, 7: e40556. PMID: 22808193, PMCID: PMC3393683, DOI: 10.1371/journal.pone.0040556.Peer-Reviewed Original ResearchConceptsSurvival advantageInsulin-like signalingAbility of mosquitoesInsulin-like peptidesInfection altersUninfected mosquitoesOocyst developmentPlasmodium developmentEnhanced expressionInsect vectorsPeriods of starvationFitness costsEnhanced survivalVertebrate hostsMicroarray analysisCarbohydrate catabolismCause malariaPlasmodiumStarvationMosquitoes
2011
Ixodes scapularis salivary gland protein P11 facilitates migration of Anaplasma phagocytophilum from the tick gut to salivary glands
Liu L, Narasimhan S, Dai J, Zhang L, Cheng G, Fikrig E. Ixodes scapularis salivary gland protein P11 facilitates migration of Anaplasma phagocytophilum from the tick gut to salivary glands. EMBO Reports 2011, 12: 1196-1203. PMID: 21921936, PMCID: PMC3207102, DOI: 10.1038/embor.2011.177.Peer-Reviewed Original Research
2010
Tick Histamine Release Factor Is Critical for Ixodes scapularis Engorgement and Transmission of the Lyme Disease Agent
Dai J, Narasimhan S, Zhang L, Liu L, Wang P, Fikrig E. Tick Histamine Release Factor Is Critical for Ixodes scapularis Engorgement and Transmission of the Lyme Disease Agent. PLOS Pathogens 2010, 6: e1001205. PMID: 21124826, PMCID: PMC2991271, DOI: 10.1371/journal.ppat.1001205.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiomarkers, TumorBlotting, WesternBorrelia burgdorferiFeeding BehaviorFemaleHistamineHumansImmunizationIxodesLyme DiseaseMiceMice, Inbred C3HReverse Transcriptase Polymerase Chain ReactionRNA, MessengerRNA, Small InterferingSalivaTick InfestationsTumor Protein, Translationally-Controlled 1ConceptsTick-borne pathogensB. burgdorferi transmissionTick engorgementB. burgdorferi burdenHistamine-releasing factorRapid feeding phaseBurgdorferi-infected ticksAnimal healthTick feedingTick salivaDiverse infectious agentsDisease agentsTicksIxodes scapularisLyme disease agentRNA interferenceFeeding phaseVaccine potentialQuantitative reverse transcription PCRReverse transcription-PCRHistamine releaseEffective vaccineVascular permeabilityBlood flowInfectious agents
2008
Extrinsic incubation periods for horizontal and vertical transmission of West Nile virus by Culex pipiens pipiens (Diptera: Culicidae).
Anderson J, Main A, Delroux K, Fikrig E. Extrinsic incubation periods for horizontal and vertical transmission of West Nile virus by Culex pipiens pipiens (Diptera: Culicidae). Journal Of Medical Entomology 2008, 45: 445-51. PMID: 18533438, DOI: 10.1603/0022-2585(2008)45[445:eipfha]2.0.co;2.Peer-Reviewed Original ResearchConceptsWest Nile virusInfection rateVertical transmissionNile virusMinimal infection rateHighest minimum infection rateVertical transmission rateMinimum infection rateWNV 1Extrinsic incubation periodDay 13Day 16Extrinsic incubationMembrane feederCulex pipiens pipiens L.Horizontal transmission ratesInitiation of amplificationFirst bloodmealVirusSurvival of virusesMiceFemalesHamstersHorizontal transmissionWNVExtrinsic Incubation Periods for Horizontal and Vertical Transmission of West Nile Virus by Culex pipiens pipiens (Diptera: Culicidae)
Anderson JF, Main AJ, Delroux K, Fikrig E. Extrinsic Incubation Periods for Horizontal and Vertical Transmission of West Nile Virus by Culex pipiens pipiens (Diptera: Culicidae). Journal Of Medical Entomology 2008, 45: 445-451. DOI: 10.1093/jmedent/45.3.445.Peer-Reviewed Original ResearchConceptsWest Nile virusInfection rateVertical transmissionNile virusMinimal infection rateHighest minimum infection rateVertical transmission rateMinimum infection rateWNV 1Extrinsic incubation periodDay 13Day 16Extrinsic incubationMembrane feederHorizontal transmission ratesCulex pipiens pipiens L.Initiation of amplificationFirst bloodmealVirusSurvival of virusesMiceFemalesHamstersHorizontal transmissionWNV
2007
Immunity against Ixodes scapularis Salivary Proteins Expressed within 24 Hours of Attachment Thwarts Tick Feeding and Impairs Borrelia Transmission
Narasimhan S, DePonte K, Marcantonio N, Liang X, Royce TE, Nelson KF, Booth CJ, Koski B, Anderson JF, Kantor F, Fikrig E. Immunity against Ixodes scapularis Salivary Proteins Expressed within 24 Hours of Attachment Thwarts Tick Feeding and Impairs Borrelia Transmission. PLOS ONE 2007, 2: e451. PMID: 17505544, PMCID: PMC1866177, DOI: 10.1371/journal.pone.0000451.Peer-Reviewed Original Research
2002
Examination of the Borrelia burgdorferi Transcriptome in Ixodes scapularis during Feeding
Narasimhan S, Santiago F, Koski RA, Brei B, Anderson JF, Fish D, Fikrig E. Examination of the Borrelia burgdorferi Transcriptome in Ixodes scapularis during Feeding. Journal Of Bacteriology 2002, 184: 3122-3125. PMID: 12003955, PMCID: PMC135063, DOI: 10.1128/jb.184.11.3122-3125.2002.Peer-Reviewed Original ResearchConceptsBorrelia burgdorferi gene expressionB. burgdorferi genesPeriplasmic proteinsSignal transductionChromosomal genesPutative lipoproteinSubstrate transportGene expressionDifferential expressionGenesEnergy metabolismIxodes scapularis ticksGlobal analysisIxodes scapularisScapularis ticksExpressionTranscriptomeTransductionTicksProteinFeedingMetabolismScapularis
2000
SALP16, a gene induced in Ixodes scapularis salivary glands during tick feeding.
Das S, Marcantonio N, Deponte K, Telford SR, Anderson JF, Kantor FS, Fikrig E. SALP16, a gene induced in Ixodes scapularis salivary glands during tick feeding. American Journal Of Tropical Medicine And Hygiene 2000, 62: 99-105. PMID: 10761732, DOI: 10.4269/ajtmh.2000.62.99.Peer-Reviewed Original ResearchAmino Acid SequenceAnimalsBase SequenceBlotting, WesternEnzyme-Linked Immunosorbent AssayFeeding BehaviorFemaleGene Expression RegulationGene LibraryGuinea PigsIxodesMiceMice, Inbred C3HMolecular Sequence DataPolymerase Chain ReactionRandom Amplified Polymorphic DNA TechniqueRecombinant ProteinsRNASalivary GlandsSalivary Proteins and PeptidesSequence Analysis, DNATick Infestations
1995
Growth and migration of Borrelia burgdorferi in Ixodes ticks during blood feeding.
De Silva AM, Fikrig E. Growth and migration of Borrelia burgdorferi in Ixodes ticks during blood feeding. American Journal Of Tropical Medicine And Hygiene 1995, 53: 397-404. PMID: 7485694, DOI: 10.4269/ajtmh.1995.53.397.Peer-Reviewed Original Research