2013
Mosquito Saliva Serine Protease Enhances Dissemination of Dengue Virus into the Mammalian Host
Conway MJ, Watson AM, Colpitts TM, Dragovic SM, Li Z, Wang P, Feitosa F, Shepherd DT, Ryman KD, Klimstra WB, Anderson JF, Fikrig E. Mosquito Saliva Serine Protease Enhances Dissemination of Dengue Virus into the Mammalian Host. Journal Of Virology 2013, 88: 164-175. PMID: 24131723, PMCID: PMC3911723, DOI: 10.1128/jvi.02235-13.Peer-Reviewed Original ResearchConceptsDengue virusDENV infectivityAedes aegypti salivaMurine lymph nodesSalivary gland extractsAegypti salivaPrevention of diseaseLymph nodesMosquito salivaDENV loadVivo modelSerine protease activityNovel targetViral attachmentViral infectivityVirus infectivitySerine protease inhibitorHeparan sulfate proteoglycanExtracellular matrix proteinsRNA knockdownProtease inhibitorsInfectivitySulfate proteoglycanCell migrationMammalian hosts
2002
A novel family of anticoagulants from the saliva of Ixodes scapularis
Narasimhan S, Koski RA, Beaulieu B, Anderson JF, Ramamoorthi N, Kantor F, Cappello M, Fikrig E. A novel family of anticoagulants from the saliva of Ixodes scapularis. Insect Molecular Biology 2002, 11: 641-650. PMID: 12421422, DOI: 10.1046/j.1365-2583.2002.00375.x.Peer-Reviewed Original ResearchConceptsN-terminal amino acid sequenceSalivary gland cDNA libraryAmino acid sequenceN-terminal sequenceCDNA libraryAcid sequenceAnticoagulant proteinsIxodes scapularisMolecular approachesTerminal sequenceIntrinsic pathwayEscherichia coliSpecific inhibitorNovel familyProteinSalp14Different functionsTick salivaSequenceBiological activityDose-dependent mannerProtease inhibitorsReversed-phase HPLCParaloguesScapularis
2000
Attachment of Borrelia burgdorferi within Ixodes scapularis mediated by outer surface protein A
Pal U, de Silva A, Montgomery R, Fish D, Anguita J, Anderson J, Lobet Y, Fikrig E. Attachment of Borrelia burgdorferi within Ixodes scapularis mediated by outer surface protein A. Journal Of Clinical Investigation 2000, 106: 561-569. PMID: 10953031, PMCID: PMC380253, DOI: 10.1172/jci9427.Peer-Reviewed Original ResearchGranulocytic Ehrlichiosis in Mice Deficient in Phagocyte Oxidase or Inducible Nitric Oxide Synthase
Banerjee R, Anguita J, Fikrig E. Granulocytic Ehrlichiosis in Mice Deficient in Phagocyte Oxidase or Inducible Nitric Oxide Synthase. Infection And Immunity 2000, 68: 4361-4362. PMID: 10858261, PMCID: PMC101771, DOI: 10.1128/iai.68.7.4361-4362.2000.Peer-Reviewed Original Research
1995
Borrelia burgdorferi genes selectively expressed in the infected host.
Suk K, Das S, Sun W, Jwang B, Barthold SW, Flavell RA, Fikrig E. Borrelia burgdorferi genes selectively expressed in the infected host. Proceedings Of The National Academy Of Sciences Of The United States Of America 1995, 92: 4269-4273. PMID: 7753795, PMCID: PMC41925, DOI: 10.1073/pnas.92.10.4269.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAntibodies, BacterialAntigen-Antibody ReactionsAntigens, BacterialBacterial VaccinesBase SequenceBlotting, NorthernBorrelia burgdorferi GroupDNA PrimersDNA, BacterialGene ExpressionGene Transfer TechniquesGenes, BacterialGenomic LibraryImmunizationMiceMice, Inbred C3HMolecular Sequence DataPolymerase Chain ReactionSequence Homology, Amino AcidConceptsInfected hostRNA PCRDifferential screeningScreening strategyExpression libraryB. burgdorferiSelective expressionNorthern blot analysisBlot analysisMiceBorrelia burgdorferi genesB. burgdorferi genesVivoGene transfer systemMicrobial genesScreening methodPathogenic microorganismsScreeningGenesAntigen