2012
SERUM ANTIBODIES TO WHOLE-CELL AND RECOMBINANT ANTIGENS OF BORRELIA BURGDORFERI IN COTTONTAIL RABBITS
Magnarelli LA, Norris SJ, Fikrig E. SERUM ANTIBODIES TO WHOLE-CELL AND RECOMBINANT ANTIGENS OF BORRELIA BURGDORFERI IN COTTONTAIL RABBITS. Journal Of Wildlife Diseases 2012, 48: 12-20. PMID: 22247369, PMCID: PMC3261588, DOI: 10.7589/0090-3558-48.1.12.Peer-Reviewed Original Research
2006
ANTIBODIES TO WHOLE-CELL OR RECOMBINANT ANTIGENS OF BORRELIA BURGDORFERI, ANAPLASMA PHAGOCYTOPHILUM, AND BABESIA MICROTI IN WHITE-FOOTED MICE
Magnarelli LA, Stafford KC, IJdo JW, Fikrig E. ANTIBODIES TO WHOLE-CELL OR RECOMBINANT ANTIGENS OF BORRELIA BURGDORFERI, ANAPLASMA PHAGOCYTOPHILUM, AND BABESIA MICROTI IN WHITE-FOOTED MICE. Journal Of Wildlife Diseases 2006, 42: 732-738. PMID: 17255439, DOI: 10.7589/0090-3558-42.4.732.Peer-Reviewed Original ResearchMeSH KeywordsAnaplasma phagocytophilumAnimalsAnimals, WildAntibodies, BacterialAntibodies, ProtozoanAntigens, BacterialAntigens, ProtozoanBabesia microtiBabesiosisBorrelia burgdorferiConnecticutEhrlichiosisEnzyme-Linked Immunosorbent AssayLyme DiseasePeromyscusRodent DiseasesSeroepidemiologic StudiesTick-Borne DiseasesConceptsEnzyme-linked immunosorbent assayB. burgdorferiOverall antibody prevalence rateBabesia microtiBorrelia burgdorferiAntibody prevalence ratesAnaplasma phagocytophilumA. phagocytophilumIndirect fluorescent antibodyWhite-footed miceWhole cell antigenTick-infested areasWhole-cell B. burgdorferiCurrent infectionPrevalence ratesMouse serumAntigenImmunosorbent assaySerum samplesRecombinant antigensAntibodiesSerumB. microtiMiceFluorescent antibody
2005
Seroprevalence of antibodies against Borrelia burgdorferi and Anaplasma phagocytophilum in cats.
Magnarelli LA, Bushmich SL, IJdo JW, Fikrig E. Seroprevalence of antibodies against Borrelia burgdorferi and Anaplasma phagocytophilum in cats. American Journal Of Veterinary Research 2005, 66: 1895-9. PMID: 16334946, DOI: 10.2460/ajvr.2005.66.1895.Peer-Reviewed Original ResearchConceptsRecombinant antigensSerum antibodiesB. burgdorferiHealthy catsBorrelia burgdorferiSeroprevalence of antibodiesWestern blot testingAnaplasma phagocytophilumA. phagocytophilumB. burgdorferi antigensWhole cell antigenA. phagocytophilum infectionIndirect fluorescent antibody stainingWestern blot analysisIxodes scapularis ticksSeropositivity rateWhole-cell B. burgdorferiSerum reactivityIll catsFluorescent antibody stainingClinical relevanceSera reactiveSpecific recombinant antigensMost catsOuter surface proteinsDetection of antibodies to Borrelia burgdorferi in naturally infected horses in the USA by enzyme-linked immunosorbent assay using whole-cell and recombinant antigens
Magnarelli L, Fikrig E. Detection of antibodies to Borrelia burgdorferi in naturally infected horses in the USA by enzyme-linked immunosorbent assay using whole-cell and recombinant antigens. Research In Veterinary Science 2005, 79: 99-103. PMID: 15924926, DOI: 10.1016/j.rvsc.2004.11.009.Peer-Reviewed Original Research
2004
A comparison of serologic tests for the detection of serum antibodies to whole-cell and recombinant Borrelia burgdorferi antigens in cattle.
Magnarelli LA, Bushmich SL, Sherman BA, Fikrig E. A comparison of serologic tests for the detection of serum antibodies to whole-cell and recombinant Borrelia burgdorferi antigens in cattle. Canadian Veterinary Journal 2004, 45: 667-73. PMID: 15368740, PMCID: PMC546445.Peer-Reviewed Original ResearchMeSH KeywordsAnaplasma phagocytophilumAnimalsAntibodies, BacterialAntigens, BacterialBlotting, WesternBorrelia burgdorferiCattleCattle DiseasesConnecticutEnzyme-Linked Immunosorbent AssayFemaleFluorescent Antibody Technique, IndirectLyme DiseaseMaleMolecular WeightRecombinant ProteinsReproducibility of ResultsSensitivity and SpecificityTick InfestationsConceptsPolyvalent enzyme-linked immunosorbentVlsE antigenBorrelia burgdorferi antigensRecombinant antigensAntibody staining methodsHealthy dairyB. burgdorferi antigensIndirect fluorescent-antibody staining methodsCattle seraHigh specificityLeptospira interrogans serovarsBrucella spWhole cell antigenSerum samplesWestern blot procedureP39 antigen
2001
Reactivity of dog sera to whole-cell or recombinant antigens of Borrelia burgdorferi by ELISA and immunoblot analysis
Magnarelli LA, Levy SA, Ijdo JW, Wu C, Padula SJ, Fikrig E. Reactivity of dog sera to whole-cell or recombinant antigens of Borrelia burgdorferi by ELISA and immunoblot analysis. Journal Of Medical Microbiology 2001, 50: 889-895. PMID: 11599738, DOI: 10.1099/0022-1317-50-10-889.Peer-Reviewed Original ResearchConceptsEnzyme-linked immunosorbent assayWhole cell antigenRecombinant antigensB. burgdorferiNon-vaccinated dogsPolyvalent enzyme-linked immunosorbent assaysCanine borreliosisTick-infested areasTotal immunoglobulinWhole-cell B. burgdorferiAntibody titresBorrelia burgdorferi sensu strictoVaccinated dogsJoint disordersImmune responseBurgdorferi sensu strictoAntigenImmunosorbent assaySerumDogsNatural infectionRecombinant Protein-44-Based Class-Specific Enzyme-Linked Immunosorbent Assays for Serologic Diagnosis of Human Granulocytic Ehrlichiosis
Magnarelli L, IJdo J, Wu C, Fikrig E. Recombinant Protein-44-Based Class-Specific Enzyme-Linked Immunosorbent Assays for Serologic Diagnosis of Human Granulocytic Ehrlichiosis. European Journal Of Clinical Microbiology & Infectious Diseases 2001, 20: 482-485. PMID: 11561804, DOI: 10.1007/s100960100542.Peer-Reviewed Original ResearchConceptsPolyvalent enzyme-linked immunosorbent assaysClass-specific enzyme-linked immunosorbent assaysEnzyme-linked immunosorbent assayIndirect fluorescent-antibody analysisFluorescent antibody analysisImmunosorbent assaySpecificity testsIgG antibodiesHigh sensitivityHuman granulocytic ehrlichiosisHuman serumGranulocytic ehrlichiosisWhole cell antigenAntibody analysisImmunoglobulin M antibodiesOnset of illnessSerologic diagnosisSerum samplesHuman granulocytic ehrlichiosis (HGE) agentPatient 3False-positive resultsIgM antibodiesM antibodiesTotal immunoglobulinTotal antibodyEvaluation of a polyvalent enzyme-linked immunosorbent assay incorporating a recombinant p44 antigen for diagnosis of granulocytic ehrlichiosis in dogs and horses.
Magnarelli LA, Ijdo JW, Van Andel AE, Wu C, Fikrig E. Evaluation of a polyvalent enzyme-linked immunosorbent assay incorporating a recombinant p44 antigen for diagnosis of granulocytic ehrlichiosis in dogs and horses. American Journal Of Veterinary Research 2001, 62: 29-32. PMID: 11197555, DOI: 10.2460/ajvr.2001.62.29.Peer-Reviewed Original ResearchConceptsEquine samplesPolyvalent ELISAPolyvalent enzyme-linked immunosorbentGranulocytic ehrlichiosisIFA stainingEquine serum samplesE risticiiEhrlichia canisCanine serum samplesCanine samplesSpecific recombinant antigensE equiHorsesEhrlichiosisPercentage of samplesIndirect fluorescent antibody stainingRecombinant antigensDogsWhole cell antigenCanisEquiELISA resultsSerum samplesPresent studyFluorescent antibody staining
2000
Serologic Diagnosis of Lyme Borreliosis by Using Enzyme-Linked Immunosorbent Assays with Recombinant Antigens
Magnarelli L, Ijdo J, Padula S, Flavell R, Fikrig E. Serologic Diagnosis of Lyme Borreliosis by Using Enzyme-Linked Immunosorbent Assays with Recombinant Antigens. Journal Of Clinical Microbiology 2000, 38: 1735-1739. PMID: 10790090, PMCID: PMC86574, DOI: 10.1128/jcm.38.5.1735-1739.2000.Peer-Reviewed Original ResearchConceptsEnzyme-linked immunosorbent assayHuman granulocytic ehrlichiosisRecombinant antigensClass-specific enzyme-linked immunosorbent assaysIgG antibodiesAntibody reactivityLyme borreliosisSerum samplesImmunoblotting of seraImmunoglobulin M antibodiesLow antibody titersB. burgdorferiEnzyme-linked immunosorbentWhole cell antigenAnalysis of serumWestern blot analysisOuter surface protein CErythema migransRheumatoid arthritisAntibody titersIgM antibodiesM antibodiesBorrelia burgdorferi sensu strictoOral infectionSurface protein C
1997
Serologic diagnosis of canine and equine borreliosis: use of recombinant antigens in enzyme-linked immunosorbent assays
Magnarelli LA, Flavell RA, Padula SJ, Anderson JF, Fikrig E. Serologic diagnosis of canine and equine borreliosis: use of recombinant antigens in enzyme-linked immunosorbent assays. Journal Of Clinical Microbiology 1997, 35: 169-173. PMID: 8968901, PMCID: PMC229532, DOI: 10.1128/jcm.35.1.169-173.1997.Peer-Reviewed Original ResearchConceptsRecombinant antigensPolyvalent enzyme-linked immunosorbent assaysWhole-cell B. burgdorferiEnzyme-linked immunosorbent assayWestern blot analysisOuter surface protein ASensu strictoBlot analysisWhole cellsProtein AOSPFSerologic diagnosisBorrelia burgdorferi sensu strictoEquid seraBurgdorferi sensu strictoB. burgdorferiSurface protein AP41OspCWhole cell antigenEquine serumDog serumImmunosorbent assayOspBSerum samples
1995
REACTIVITY OF WHITE-FOOTED MOUSE AND RACCOON SERA TO WHOLE CELL AND RECOMBINANT ANTIGENS OF BORRELIA BURGDORFERI
Magnarelli L, Anderson J, Flavell R, Fikrig E. REACTIVITY OF WHITE-FOOTED MOUSE AND RACCOON SERA TO WHOLE CELL AND RECOMBINANT ANTIGENS OF BORRELIA BURGDORFERI. Journal Of Wildlife Diseases 1995, 31: 339-344. PMID: 8592354, DOI: 10.7589/0090-3558-31.3.339.Peer-Reviewed Original ResearchConceptsWhole cell antigenCell antigensRecombinant antigensSpecific recombinant antigensGeometric mean antibody titersMean antibody titersPresence of antibodiesB. burgdorferi infectionEnzyme-linked immunosorbentWhite-footed miceAntibody titersBorrelia burgdorferi sensu strictoBurgdorferi infectionBurgdorferi sensu stricto