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NIAAA R24 Alcohol Research Resources

Mouse models for alcohol metabolism and tissue injury

Major enzymatic pathways involved in ethanol (EtOH), glutathione (GSH) and homocysteine (Hcys) metabolism. CAT, catalase; ADH, alcohol dehydrogenase; CYP2E1, cytochrome P450 2E1; ALDH, aldehyde dehydrogenase; GCL, glutamate-cysteine ligase; GCLC, catalytic subunit of GCL; GCLM, modifier subunit of GCL; -GC, -glutamylcysteine; Glu, L-glutamate; Gly, glycine; GSSG, glutathione disulfide; NRF2, Nuclear factor erythroid 2-related factor 2; SAM, S-adenosyl-methionine.

Dr. Vasiliou’s laboratory maintains transgenic animal models that are unique and have high relevance to alcohol research. These mice are readily available to researchers by request (see left column). These include:

a) Aldh1a1, Aldh2, Aldh1b1, Cat, Cyp2e1, Adh3a1 and Gclm single knockouts (KO)

b) Aldh1a1 and Aldh3a1 knock-in lines (both have mutated the Cys-302 of the active site)

c) Aldh1a1/Aldh1b1, Aldh1b1/Aldh2, Cat/Cyp2e1 and Gclm/Nrf2 double knockouts (DKO)

d) Conditional Gclc floxed strain

e) Hepatocyte-specific Gclc knockout

Funded by NIAAA (R24AA022057)


Please send inquiries and requests to:
Dr. Vasilis Vasiliou.