2016
Electrochemical detection of DNA 3′-phosphatases based on surface-extended DNA nanotail strategy
Wu D, Li C, Hu X, Mao X, Li G. Electrochemical detection of DNA 3′-phosphatases based on surface-extended DNA nanotail strategy. Analytica Chimica Acta 2016, 924: 29-34. PMID: 27181641, DOI: 10.1016/j.aca.2016.04.013.Peer-Reviewed Original ResearchConceptsDNA 3'-phosphataseT4 polynucleotide kinase phosphatasePolynucleotide kinase phosphataseLow detection limitLong DNA strandsSubstrate DNACellular processesTerminal deoxynucleotidyl transferaseDNA probesDNA strandsGold electrodeDephosphorylation reactionsTerminal hydroxylElectrochemical detectionUltrasensitive detectionDetection limitDNAAnalytical performanceDeoxynucleotidyl transferaseSignal amplificationDrug discoveryDephosphorylationClinical diagnosticsSignal enhancementTdT
2008
Rv2131c from Mycobacterium tuberculosis Is a CysQ 3′-Phosphoadenosine-5′-phosphatase
Hatzios SK, Iavarone AT, Bertozzi CR. Rv2131c from Mycobacterium tuberculosis Is a CysQ 3′-Phosphoadenosine-5′-phosphatase. Biochemistry 2008, 47: 5823-5831. PMID: 18454554, PMCID: PMC2711008, DOI: 10.1021/bi702453s.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceBacterial ProteinsBiochemistryEscherichia coliHydrogen-Ion ConcentrationKineticsMolecular Sequence DataMutationMycobacterium tuberculosisNucleotidasesPhosphoadenosine PhosphosulfatePhosphoric Monoester HydrolasesProtein BindingSequence Homology, Amino AcidSpectrometry, Mass, Electrospray IonizationConceptsSulfur-containing metabolitesSulfate assimilation pathwayRecent biochemical studiesMagnesium-dependent mannerFunctional complementationAssimilation pathwayMyo-inositol 1Protein functionSulfur metabolismBioinformatics analysisMass spectrometry-based assayImportant regulatorBiochemical studiesCysQMtb genomePrimary substrateEnzymeOptimal activityK catPathwayMM MgCl 2GenomeComplementationMetabolitesMutants
2004
Expression of P2Y nucleotide receptors and ectonucleotidases in quiescent and activated rat hepatic stellate cells
Dranoff JA, Ogawa M, Kruglov EA, Gaça M, Sévigny J, Robson SC, Wells RG. Expression of P2Y nucleotide receptors and ectonucleotidases in quiescent and activated rat hepatic stellate cells. AJP Gastrointestinal And Liver Physiology 2004, 287: g417-g424. PMID: 14764443, PMCID: PMC5241161, DOI: 10.1152/ajpgi.00294.2003.Peer-Reviewed Original ResearchConceptsHepatic stellate cellsActivated hepatic stellate cellsQuiescent hepatic stellate cellsP2Y receptorsExtracellular nucleotidesP2Y subtypesStellate cellsMRNA expressionRT-PCRInhibitor suraminP2Y receptor subtypesReceptor subtype expressionExpression of mediatorsExpression of receptorsFunctional P2Y receptorsRat hepatic stellate cellsReal-time RT-PCRP2Y nucleotide receptorsEctonucleotidase expressionLiver fibrosisReceptor subtypesSubtype expressionEctonucleotidase activityExtracellular UDPFibrogenic cells
1989
Protection of the kidney against ischemic injury by inhibition of 5'-nucleotidase
Van Waarde A, Stromski M, Thulin G, Gaudio K, Kashgarian M, Shulman R, Siegel N. Protection of the kidney against ischemic injury by inhibition of 5'-nucleotidase. American Journal Of Physiology 1989, 256: f298-f305. PMID: 2537026, DOI: 10.1152/ajprenal.1989.256.2.f298.Peer-Reviewed Original ResearchConceptsRenal ischemic insultResidual nucleotide poolMin of ischemiaRenal ATP contentFunctional recoveryIschemic injuryIschemic insultKidney functionRenal ischemiaIntramuscular injectionUntreated ratsRapid initial recoveryATP restorationRat kidneyRenal ATPIschemiaInitial recoveryKidneyRatsATP contentInsultFunctional consequencesNucleotide poolMagnetic resonanceAMPCP
1987
Aortic endothelial cell proteoheparan sulfate. I. Isolation and characterization of plasmamembrane-associated and extracellular species.
Keller R, Silbert J, Furthmayr H, Madri J. Aortic endothelial cell proteoheparan sulfate. I. Isolation and characterization of plasmamembrane-associated and extracellular species. American Journal Of Pathology 1987, 128: 286-98. PMID: 3039849, PMCID: PMC1899622.Peer-Reviewed Original ResearchConceptsDaltons apparent molecular weightProteoheparan sulfate speciesHeparan sulfate chainsApparent molecular weightHS IBovine aortic endothelial cellsSulfate chainsCultured bovine aortic endothelial cellsCore proteinAortic endothelial cellsCell surface localizationPlasma membrane fractionEndothelial cellsPulse-chase experimentsCsCl density centrifugationThird speciesPlasma membraneSubcellular fractionationExtracellular speciesSpecialized functionsMatrix localizationSurface localizationMembrane fractionSulfate biosynthesisMedium species
1979
Effect of chlorpromazine on hepatic perfusion and bile secretory function in the isolated perfused rat liver.
Tavoloni N, Reed J, Hruban Z, Boyer J. Effect of chlorpromazine on hepatic perfusion and bile secretory function in the isolated perfused rat liver. Translational Research 1979, 94: 726-41. PMID: 227976.Peer-Reviewed Original ResearchConceptsHepatic perfusionPerfusate flowBile secretionBile acid-independent bile secretionSecretory functionBile acid excretionBile secretory functionRat liverEffect of chlorpromazineLiver 20 minLobar distributionHepatic lobeAcid excretionBaseline valuesPerfusate concentrationConstant infusionHepatic ultrastructureBile acidsControl valuesCPZSodium taurocholateLiverPerfusionGM-1Liver plasma membranes
1977
Effects of chronic choleretic infusions of bile acids on the membrane of the bile canaliculus. A biochemical and morphologic study.
Nemchausky B, Layden T, Boyer J. Effects of chronic choleretic infusions of bile acids on the membrane of the bile canaliculus. A biochemical and morphologic study. Laboratory Investigation 1977, 36: 259-67. PMID: 138767.Peer-Reviewed Original Research
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