2012
Hypoxia sensing by Fe2+/α‐KG dependent dioxygenases regulate hmU synthesis in trypanosome DNA, chromatin structure and Pol II transcription
sabatini R, cliffe L, ekanayake D, Hirsch G. Hypoxia sensing by Fe2+/α‐KG dependent dioxygenases regulate hmU synthesis in trypanosome DNA, chromatin structure and Pol II transcription. The FASEB Journal 2012, 26: 535.16-535.16. DOI: 10.1096/fasebj.26.1_supplement.535.16.Peer-Reviewed Original ResearchThymidine hydroxylaseBase JIncreased parasite virulenceIncreased Pol II occupancyPol II transcription initiationGene expressionRegulation of gene expressionPol II occupancyPol II transcriptionGenome-wide changesSynthetic DNA substratesParasite virulenceGrowth of T. cruziOxygen-dependent mannerIncreased histone acetylationTranscription initiationDioxygenase superfamilyII transcriptionChromatin structureDNA substratesTranscriptional regulationIn vivo analysisPromoter regionHistone acetylationHost environment
2011
Epigenetic Regulation of Polymerase II Transcription Initiation in Trypanosoma cruzi: Modulation of Nucleosome Abundance, Histone Modification, and Polymerase Occupancy by O-Linked Thymine DNA Glucosylation
Ekanayake D, Sabatini R. Epigenetic Regulation of Polymerase II Transcription Initiation in Trypanosoma cruzi: Modulation of Nucleosome Abundance, Histone Modification, and Polymerase Occupancy by O-Linked Thymine DNA Glucosylation. MSphere 2011, 10: 1465-1472. PMID: 21926332, PMCID: PMC3209055, DOI: 10.1128/ec.05185-11.Peer-Reviewed Original ResearchConceptsPolycistronic transcription unitsBase JPromoter regionTranscription initiationChromatin structureGene expressionRNA polymerase (Pol) IIEpigenetic regulationPolymerase (Pol) IIIncreased Pol II occupancyPol II transcription initiationPol II occupancyRegulate gene expressionThymidine hydroxylasePolymerase occupancyGene organizationTrypanosome genomeTranscription unitHistone modificationsPosttranscriptional processesDNA modificationsTranscription rateHistone H3/H4 acetylationGenesH3/H4 acetylationEpigenetic Regulation of Transcription and Virulence in Trypanosoma cruzi by O-Linked Thymine Glucosylation of DNA
Ekanayake D, Minning T, Weatherly B, Gunasekera K, Nilsson D, Tarleton R, Ochsenreiter T, Sabatini R. Epigenetic Regulation of Transcription and Virulence in Trypanosoma cruzi by O-Linked Thymine Glucosylation of DNA. Molecular And Cellular Biology 2011, 31: 1690-1700. PMID: 21321080, PMCID: PMC3126337, DOI: 10.1128/mcb.01277-10.Peer-Reviewed Original ResearchConceptsPolycistronic unitsBase JTranscription initiationPol II transcription initiationSite of transcription initiationGene expressionEpigenetic regulation of transcriptionPolycistronic gene clustersProtein-coding genesPol II transcriptionGenome-wide increaseRegulation of transcriptionHost cell invasionRegulate gene expressionDNA basePolymerase IIVirulence genesGene clusterPol IIII transcriptionAffected genesPosttranscriptional processesEpigenetic regulationBiological roleEpigenetic factors
2007
Telomeric co-localization of the modified base J and contingency genes in the protozoan parasite Trypanosoma cruzi
Ekanayake D, Cipriano M, Sabatini R. Telomeric co-localization of the modified base J and contingency genes in the protozoan parasite Trypanosoma cruzi. Nucleic Acids Research 2007, 35: 6367-6377. PMID: 17881368, PMCID: PMC2095807, DOI: 10.1093/nar/gkm693.Peer-Reviewed Original ResearchConceptsTelomeric repeat sequencesBase JVariant surface glycoproteinRepeat sequencesGenes adjacent to telomeresExpression sitesAnalysis of DNA sequencesBeta-d-glucosylhydroxymethyluracilAdjacent to telomeresGene expression sitesSouth American trypanosomesSurface glycoprotein genesActive expression siteContingency genesSubtelomeric regionsTelomeric repeatsDNA sequencesAmerican trypanosomesDNA digestionTelomeric genesTrypanosoma bruceiSouthern blottingTelomere distributionKinetoplastid parasitesGlycoprotein geneJBP2, a SWI2/SNF2-like protein, regulates de novo telomeric DNA glycosylation in bloodstream form Trypanosoma brucei
Kieft R, Brand V, Ekanayake D, Sweeney K, DiPaolo C, Reznikoff W, Sabatini R. JBP2, a SWI2/SNF2-like protein, regulates de novo telomeric DNA glycosylation in bloodstream form Trypanosoma brucei. Molecular And Biochemical Parasitology 2007, 156: 24-31. PMID: 17706299, PMCID: PMC4735730, DOI: 10.1016/j.molbiopara.2007.06.010.Peer-Reviewed Original ResearchConceptsSWI2/SNF2-like proteinsBase JRegulation of antigenic variationTelomeric variant surface glycoprotein genesAntigenic variationBloodstream form Trypanosoma bruceiVariant surface glycoprotein genesBloodstream form trypanosomesSurface glycoprotein genesTelomere arraysChromatin remodelingEpigenetic silencingJBP2Telomeric DNARegulatory mechanismsGlycoprotein geneTrypanosome DNASite-specific localizationProteinThymine baseChromatinBiosynthesisGenesDNAGlycosylation
This site is protected by hCaptcha and its Privacy Policy and Terms of Service apply