2019
RPPAs for Cell Subpopulation Analysis
Kume K, Nishizuka SS. RPPAs for Cell Subpopulation Analysis. Advances In Experimental Medicine And Biology 2019, 1188: 227-237. PMID: 31820391, DOI: 10.1007/978-981-32-9755-5_12.ChaptersConceptsDrug-tolerant persistersStem cell-associated proteinsReverse phase protein arrayTolerant cell linesCell linesPhase protein arrayDifferent cell typesCell-associated proteinsGastric cancer cell linesCell biologyProteomic profilingProteomic profilesTolerant linesCellular heterogeneityClonal populationsCancer biologyPI3KCell typesCancer cell linesProtein arraysStem cellsIndividual subpopulationsProliferative conditionsBiologyProtein
2017
p90RSK Blockade Inhibits Dual BRAF and MEK Inhibitor-Resistant Melanoma by Targeting Protein Synthesis
Theodosakis N, Micevic G, Langdon CG, Ventura A, Means R, Stern DF, Bosenberg MW. p90RSK Blockade Inhibits Dual BRAF and MEK Inhibitor-Resistant Melanoma by Targeting Protein Synthesis. Journal Of Investigative Dermatology 2017, 137: 2187-2196. PMID: 28599981, PMCID: PMC6342201, DOI: 10.1016/j.jid.2016.12.033.Peer-Reviewed Original ResearchConceptsProtein synthesisRibosomal S6 kinase (RSK) familyPatient-derived melanoma cell linesDifferential protein expressionReverse phase protein arrayPhase protein arrayTranslation complexesKinase familyBI-D1870RSK inhibitorsMelanoma cell linesProtein arraysCell proliferationInhibitor treatmentProtein expressionCell linesNew targetsHuman melanoma patientsBRAF inhibitor vemurafenibNovel BET protein proteolysis-targeting chimera exerts superior lethal activity than bromodomain inhibitor (BETi) against post-myeloproliferative neoplasm secondary (s) AML cells
Saenz DT, Fiskus W, Qian Y, Manshouri T, Rajapakshe K, Raina K, Coleman KG, Crew AP, Shen A, Mill CP, Sun B, Qiu P, Kadia TM, Pemmaraju N, DiNardo C, Kim MS, Nowak AJ, Coarfa C, Crews CM, Verstovsek S, Bhalla KN. Novel BET protein proteolysis-targeting chimera exerts superior lethal activity than bromodomain inhibitor (BETi) against post-myeloproliferative neoplasm secondary (s) AML cells. Leukemia 2017, 31: 1951-1961. PMID: 28042144, PMCID: PMC5537055, DOI: 10.1038/leu.2016.393.Peer-Reviewed Original ResearchConceptsBET protein inhibitorARV-825Messenger RNAReverse phase protein arrayPhase protein arrayRNA-seqHematopoietic progenitor cellsNormal hematopoietic progenitor cellsBET proteinsE3 ubiquitin ligase cereblonLevels of p21Extraterminal (BET) proteinsBcl-xLBromodomain inhibitorsC-MycJAK inhibitor ruxolitinibBRD4Protein arraysProgenitor cellsProtein expressionHEL92.1.7 cellsImproved survivalLeukemia burdenNSG miceProfound depletion
2015
Systematic Protein Level Regulation via Degradation Machinery Induced by Genotoxic Drugs
Kume K, Ishida K, Ikeda M, Takemoto K, Shimura T, Young L, Nishizuka SS. Systematic Protein Level Regulation via Degradation Machinery Induced by Genotoxic Drugs. Journal Of Proteome Research 2015, 15: 205-215. PMID: 26625007, DOI: 10.1021/acs.jproteome.5b00759.Peer-Reviewed Original ResearchConceptsDegradation machineryGenotoxic drugsProtein dynamicsProtein-level regulationProtein degradation systemProtein levelsDistinct proteinsApoptosis-related proteinsDegradation systemProtein arraysProteinLevel regulationProteasome inhibitorsMachineryComprehensive insightRegulationMG132Similar dynamicsPairsInhibitorsDynamicsDifferent concentrationsResponseSimilarityLevels
2010
A genomic and proteomic investigation of the impact of preimplantation factor on human decidual cells
Paidas MJ, Krikun G, Huang SJ, Jones R, Romano M, Annunziato J, Barnea ER. A genomic and proteomic investigation of the impact of preimplantation factor on human decidual cells. American Journal Of Obstetrics And Gynecology 2010, 202: 459.e1-459.e8. PMID: 20452489, PMCID: PMC2867836, DOI: 10.1016/j.ajog.2010.03.024.Peer-Reviewed Original ResearchConceptsHuman endometrial stromal cellsDown syndrome cell adhesion moleculeSyndrome cell adhesion moleculeGlobal gene expressionProtein arraysQuantitative mass spectrometryPreImplantation FactorBinding protein 1B-cell lymphoma protein 2PIF interactionProteomic analysisProteomic investigationCell adhesion moleculeInsulin-degrading enzymeGene expressionPathway analysisApoptotic pathwayApoptotic processToll-like receptor 5Protein 2Amino acid peptideProtein 1Protein 15Synthetic preimplantation factorEndometrial stromal cellsIdentification of Tumor and Invasion Suppressor Gene Modulators in Bladder Cancer by Different Classes of Histone Deacetylase Inhibitors Using Reverse Phase Protein Arrays
Gould JJ, Kenney PA, Rieger-Christ KM, Neto B, Wszolek MF, LaVoie A, Holway AH, Spurrier B, Austin J, Cammarata BK, Canes D, Libertino JA, Summerhayes IC. Identification of Tumor and Invasion Suppressor Gene Modulators in Bladder Cancer by Different Classes of Histone Deacetylase Inhibitors Using Reverse Phase Protein Arrays. Journal Of Urology 2010, 183: 2395-2402. PMID: 20403623, DOI: 10.1016/j.juro.2010.02.004.Peer-Reviewed Original ResearchConceptsReverse phase protein arrayPhase protein arrayHistone deacetylase inhibitorsInvasive cell linesDeacetylase inhibitorsProtein arraysExpression profilesCell linesProtein expression profilesMS-275Specific protein groupsSuppressor gene expressionHigh-throughput screeningGene expressionGene modulatorsTrichostatin AInvasive linesSuppressor geneBladder carcinoma cell lineProtein groupsFAK expressionCarcinoma cell linesBladder cancerValproic acidThroughput screening
2009
Phosphatidylinositol-3-Kinase as a Therapeutic Target in Melanoma
Aziz SA, Davies M, Pick E, Zito C, Jilaveanu L, Camp RL, Rimm DL, Kluger Y, Kluger HM. Phosphatidylinositol-3-Kinase as a Therapeutic Target in Melanoma. Clinical Cancer Research 2009, 15: 3029-3036. PMID: 19383818, PMCID: PMC4431617, DOI: 10.1158/1078-0432.ccr-08-2768.Peer-Reviewed Original ResearchMeSH KeywordsBrain NeoplasmsCell ProliferationChromonesEnzyme InhibitorsHumansImmunoblottingImmunoenzyme TechniquesMelanomaMorpholinesNevus, PigmentedPhosphatidylinositol 3-KinasesPhosphoinositide-3 Kinase InhibitorsPhosphorylationProtein Array AnalysisSkin NeoplasmsTissue Array AnalysisTumor Cells, CulturedConceptsPhosphatidylinositol-3 kinasePI3K inhibitorsExpression of p85PI3KP110alpha subunitPathway membersK inhibitorsCell linesPI3K pathway membersReverse phase protein arrayGood drug targetPhase protein arrayPI3K pathwayTargets of drugsCellular processesPhospho-Akt levelsPI3K inhibitionMelanoma cell linesDrug targetsFull activationP85K pathwayLY294002Protein arraysResistant cell lines
2005
Aptamer‐Directed Self‐Assembly of Protein Arrays on a DNA Nanostructure
Liu Y, Lin C, Li H, Yan H. Aptamer‐Directed Self‐Assembly of Protein Arrays on a DNA Nanostructure. Angewandte Chemie International Edition 2005, 44: 4333-4338. PMID: 15945116, DOI: 10.1002/anie.200501089.Peer-Reviewed Original Research
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