2020
Severe reactive astrocytes precipitate pathological hallmarks of Alzheimer’s disease via H2O2− production
Chun H, Im H, Kang YJ, Kim Y, Shin JH, Won W, Lim J, Ju Y, Park YM, Kim S, Lee SE, Lee J, Woo J, Hwang Y, Cho H, Jo S, Park JH, Kim D, Kim DY, Seo JS, Gwag BJ, Kim YS, Park KD, Kaang BK, Cho H, Ryu H, Lee CJ. Severe reactive astrocytes precipitate pathological hallmarks of Alzheimer’s disease via H2O2− production. Nature Neuroscience 2020, 23: 1555-1566. PMID: 33199896, DOI: 10.1038/s41593-020-00735-y.Peer-Reviewed Original ResearchConceptsReactive astrocytesAlzheimer's diseaseAPP/PS1 micePathogenesis of ADReactivity of astrocytesBrains of patientsAppropriate experimental modelsGlial activationPS1 miceAstrocytic reactivityBrain atrophyPathological featuresNeuronal deathMonoamine oxidase BPathological hallmarkAD modelPrecise molecular mechanismsAnimal modelsAstrocytesCognitive impairmentPathological contributionExperimental modelDiseaseOxidase BEventual death
2019
Chapter Eight Illuminating the virus life cycle with single-molecule FRET imaging
Lu M, Ma X, Mothes W. Chapter Eight Illuminating the virus life cycle with single-molecule FRET imaging. Advances In Virus Research 2019, 105: 239-273. PMID: 31522706, PMCID: PMC7246055, DOI: 10.1016/bs.aivir.2019.07.004.Peer-Reviewed Original ResearchConceptsVirus life cycleMolecular mechanismsViral proteinsSingle-molecule FRETBiological moleculesPrecise molecular mechanismsFörster resonance energy transfer (FRET) imagingLife cycleConformational dynamicsReal-time imagingEnergetics of transitionsNovel antiviral therapiesStructural intermediatesConformational changesConformational statesNative stateSmFRETRelevant conditionsElegant experimental designsAsymmetric intermediatesIntermediatesViral fusionViral glycoproteinsStructural methodsIntact virions
2018
Foot-and-mouth disease virus capsid protein VP2 activates the cellular EIF2S1-ATF4 pathway and induces autophagy via HSPB1
Sun P, Zhang S, Qin X, Chang X, Cui X, Li H, Zhang S, Gao H, Wang P, Zhang Z, Luo J, Li Z. Foot-and-mouth disease virus capsid protein VP2 activates the cellular EIF2S1-ATF4 pathway and induces autophagy via HSPB1. Autophagy 2018, 14: 336-346. PMID: 29166823, PMCID: PMC5902195, DOI: 10.1080/15548627.2017.1405187.Peer-Reviewed Original ResearchConceptsCapsid protein VP2FMDV infectionProtein VP2Polyglutamine expansion proteinsMacroautophagy/autophagyMammalian cell linesPrecise molecular mechanismsFMDV capsid proteinsNatural host cellsInhibition of autophagyCloven-hoofed animalsAutophagy inductionMolecular mechanismsAutophagy processHost cellsAkt-mTORMouth disease virusCapsid proteinViral replicationAutophagyFMDV replicationHSPB1InfectionCell linesVP2
2016
Integrative Analysis of RNA-Interactome and Translatome Reveal Functional Targets of MSI2 in Myeloid Leukemia
Ramirez O, Kesarwani A, Abhishek G, Minella A, Pillai M. Integrative Analysis of RNA-Interactome and Translatome Reveal Functional Targets of MSI2 in Myeloid Leukemia. Blood 2016, 128: 1881. DOI: 10.1182/blood.v128.22.1881.1881.Peer-Reviewed Original ResearchRNA binding proteinPolysome profilingCell cycle regulatorsTranscription factorsMusashi-2RNA interactomeTarget mRNAsFunctional targetAsymmetric cell divisionSpecific cellular functionsThousands of transcriptsDNA damage responseRBP-RNA complexesMinimal sequence motifSequence-specific fashionPrecise molecular mechanismsTranslational repressionTranslatome profilingCell fateTranslatome analysisCellular functionsSequence motifsDamage responseCell divisionNext-generation sequencing
2015
Imbalance of excitatory/inhibitory synaptic protein expression in iPSC-derived neurons from FOXG1+/− patients and in foxg1+/− mice
Patriarchi T, Amabile S, Frullanti E, Landucci E, Lo Rizzo C, Ariani F, Costa M, Olimpico F, W Hell J, M Vaccarino F, Renieri A, Meloni I. Imbalance of excitatory/inhibitory synaptic protein expression in iPSC-derived neurons from FOXG1+/− patients and in foxg1+/− mice. European Journal Of Human Genetics 2015, 24: 871-880. PMID: 26443267, PMCID: PMC4820038, DOI: 10.1038/ejhg.2015.216.Peer-Reviewed Original ResearchConceptsRett syndromeSynaptic markersInhibitory synapsesExcitatory/inhibitory balanceSynaptic protein expressionFetal mouse brainInhibitory synaptic markersPathogenesis of RTTExcitatory synaptic markersSevere neurodevelopmental disorderGlutamatergic markersInhibitory balanceAdult brainAdult micePrecise molecular mechanismsSynaptic differentiationPatientsMouse brainBrain synapsesPathological eventsNeuronsProtein expressionBrainGluD1Neurodevelopmental disordersComplement C1q-induced activation of β-catenin signalling causes hypertensive arterial remodelling
Sumida T, Naito AT, Nomura S, Nakagawa A, Higo T, Hashimoto A, Okada K, Sakai T, Ito M, Yamaguchi T, Oka T, Akazawa H, Lee JK, Minamino T, Offermanns S, Noda T, Botto M, Kobayashi Y, Morita H, Manabe I, Nagai T, Shiojima I, Komuro I. Complement C1q-induced activation of β-catenin signalling causes hypertensive arterial remodelling. Nature Communications 2015, 6: 6241. PMID: 25716000, PMCID: PMC4351572, DOI: 10.1038/ncomms7241.Peer-Reviewed Original ResearchConceptsVascular smooth muscle cellsProliferation of VSMCsArterial remodellingΒ-catenin signalingΒ-cateninComplement C1qBlood pressure elevationEnd-organ damageNovel therapeutic targetSmooth muscle cellsMacrophage depletionImmune cellsPrecise molecular mechanismsTherapeutic targetStructural remodellingMuscle cellsRemodellingHypertensionArteriosclerosisComplement C1ActivationC1qMolecular mechanismsSignalingGene deletion
2012
The Assembly of Human Complex I
Nouws J, Calvaruso M, Nijtmans L. The Assembly of Human Complex I. 2012, 193-217. DOI: 10.1007/978-94-007-4138-6_10.Peer-Reviewed Original ResearchComplex IHuman mitochondrial complex IComplex I assemblyHuman complex IMitochondrial complex IPrecise molecular mechanismsMitochondrial genomeSpecialized proteinsFunctional enzymeMolecular mechanismsChaperonesAssemblyEnzymeStepwise processDifferent stepsGenomeSubunitsProteinDifferent stagesMembraneComplicated process
2011
Dual roles of Munc18-1 rely on distinct binding modes of the central cavity with Stx1A and SNARE complex
Shi L, Kümmel D, Coleman J, Melia TJ, Giraudo CG. Dual roles of Munc18-1 rely on distinct binding modes of the central cavity with Stx1A and SNARE complex. Molecular Biology Of The Cell 2011, 22: 4150-4160. PMID: 21900493, PMCID: PMC3204075, DOI: 10.1091/mbc.e11-02-0150.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCalorimetryCell MembraneHeLa CellsHuman Growth HormoneHumansLiposomesMiceMicroscopy, FluorescenceMolecular ChaperonesMunc18 ProteinsNeuroendocrine CellsPC12 CellsProtein BindingProtein Interaction Domains and MotifsProtein TransportRatsRecombinant ProteinsSNARE ProteinsSyntaxin 1ThermodynamicsTitrimetryConceptsSoluble N-ethylmaleimide-sensitive factor attachment protein receptorsSyntaxin 1AMunc18-1N-ethylmaleimide-sensitive factor attachment protein receptorsSec1/Munc18 (SM) proteinsFactor attachment protein receptorsCentral cavitySNARE complex assemblyIntracellular membrane traffickingAttachment protein receptorsMunc18-1 mutantsLiposome fusionPrecise molecular mechanismsMunc18 proteinsMembrane traffickingSNARE complexChaperone functionH3 domainDistinct binding modesMembrane fusionMolecular mechanismsProtein receptorsDual functionNeuroendocrine cellsBinding modes
1989
Role of protein phosphorylation in neuronal signal transduction1
Hemmings H, Nairn A, McGuinness T, Huganir R, Greengard P. Role of protein phosphorylation in neuronal signal transduction1. The FASEB Journal 1989, 3: 1583-1592. PMID: 2493406, DOI: 10.1096/fasebj.3.5.2493406.Peer-Reviewed Original ResearchConceptsProtein phosphorylationSubstrate proteinsSignal transductionProtein kinaseMolecular mechanismsProtein phosphatase inhibitorSignal transduction processesPrecise molecular mechanismsAdditional molecular mechanismsSignal transduction1Extracellular signalsPhosphatase inhibitorAdditional phosphoproteinsPhysiological processesTransduction processesNicotinic acetylcholine receptorsPhosphorylationSynaptic transmissionNervous systemSynapsin IExcitable cellsDARPP-32TransductionKinaseNeurotransmitter release
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