2021
Diagnostic performance of a Recombinant Polymerase Amplification Test—Lateral Flow (RPA-LF) for cutaneous leishmaniasis in an endemic setting of Colombia
Cossio A, Jojoa J, del Mar Castro M, Castillo R, Osorio L, Shelite T, Saravia N, Melby P, Travi B. Diagnostic performance of a Recombinant Polymerase Amplification Test—Lateral Flow (RPA-LF) for cutaneous leishmaniasis in an endemic setting of Colombia. PLOS Neglected Tropical Diseases 2021, 15: e0009291. PMID: 33909619, PMCID: PMC8081229, DOI: 10.1371/journal.pntd.0009291.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentAdultAgedAged, 80 and overChildChild, PreschoolChromatography, AffinityColombiaCross-Sectional StudiesDNA PrimersDNA, KinetoplastDNA, ProtozoanFemaleHumansLeishmaniaLeishmaniasis, CutaneousMaleMiddle AgedMolecular Diagnostic TechniquesNucleic Acid Amplification TechniquesSensitivity and SpecificityYoung AdultConceptsCommunity health workersHealth workersCutaneous leishmaniasisRPA-LFReference centerHealth systemPrimary health facilitiesBurden of diseaseCross-sectional studyYears of ageRoutine diagnostic testsPublic health systemPositive likelihood ratioLeishmania kinetoplast DNANegative likelihood ratioLikelihood ratioUlcerated lesionsPolymerase chain reaction detectionSpecificity 89Endemic settingsEarly diagnosisHealth facilitiesDiagnostic test performanceWeek evolutionReal-time polymerase chain reaction detection
2018
Superior real‐time polymerase chain reaction detection of Babesia microti parasites in whole blood utilizing high‐copy BMN antigens as amplification targets
Grabias B, Clement J, Krause PJ, Lepore T, Kumar S. Superior real‐time polymerase chain reaction detection of Babesia microti parasites in whole blood utilizing high‐copy BMN antigens as amplification targets. Transfusion 2018, 58: 1924-1932. PMID: 29664114, DOI: 10.1111/trf.14642.Peer-Reviewed Original ResearchConceptsParasites/mLBlood donorsBlood samplesSeroreactive antigensBabesia microti parasitesWhole bloodTransfusion-transmitted infectionsParasite nucleic acidsReal-time polymerase chain reactionWhole blood samplesPolymerase chain reaction detectionPolymerase chain reactionSensitive molecular assaysBlood productsClinical characterizationRT-PCR detectionReal-time polymerase chain reaction detectionLaboratory diagnosisBabesia microtiStudy designInfected ticksDilution panelsAntigenZoonotic diseaseBlood
2012
Unusual DNA mismatch repair–deficient tumors in Lynch syndrome: a report of new cases and review of the literature
Karamurzin Y, Zeng Z, Stadler Z, Zhang L, Ouansafi I, Al-Ahmadie H, Sempoux C, Saltz L, Soslow R, O'Reilly E, Paty P, Coit D, Shia J, Klimstra D. Unusual DNA mismatch repair–deficient tumors in Lynch syndrome: a report of new cases and review of the literature. Human Pathology 2012, 43: 1677-1687. PMID: 22516243, DOI: 10.1016/j.humpath.2011.12.012.Peer-Reviewed Original ResearchConceptsUnusual tumorRepair proteinsLynch syndromeMismatch repair proteinsPathogenic germ-line mutationsGerm-line mutationsWell-differentiated neuroendocrine tumorsPancreatic acinar cell carcinomaPancreatic well-differentiated neuroendocrine tumorAcinar cell carcinomaMicrosatellite instabilityDNA mismatch repair proteinsDetection of microsatellite instabilityPolymerase chain reaction detectionPolymerase chain reaction testMutation informationDNA mismatch repair deficient tumorsIdentification of tumorsMismatch repair-deficient tumorsMismatch repair genesNeuroendocrine tumorsAdrenocortical carcinomaAppendiceal carcinomaDetect Lynch syndromePeritoneal mesothelioma
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