2025
An in vivo screen identifies NAT10 as a master regulator of brain metastasis
Chen J, Xu P, Cai W, Chen H, Wingrove E, Shi X, Li W, Biancon G, Zhang M, Balabaki A, Krop E, Asare E, Zhang Y, Yin M, Tebaldi T, Meier J, Westbrook T, Halene S, Liu Y, Shen H, Nguyen D, Yan Q. An in vivo screen identifies NAT10 as a master regulator of brain metastasis. Science Advances 2025, 11: eads6021. PMID: 40138393, PMCID: PMC11939035, DOI: 10.1126/sciadv.ads6021.Peer-Reviewed Original ResearchConceptsPhosphoserine aminotransferase 1Metastasis in vivoIn vivo screeningRNA helicase domainRegulator of brain metastasisMetastatic breast cancer cellsBrain metastasis in vivoBrain metastasesRNA helicaseCell growth in vitroBreast cancer cellsCancer cell proliferationSerine biosynthesisEpigenetic regulationGrowth in vitroNAT10Migration in vitroCancer cellsTumor growthCell proliferationPrimary tumor growthDrivers of brain metastasesRNACancer metastasisCancer-related deaths
2024
Unique Spatial Transcriptomic Profiling of the Murine Femoral Fracture Callus: A Preliminary Report
Jiang W, Caruana D, Back J, Lee F. Unique Spatial Transcriptomic Profiling of the Murine Femoral Fracture Callus: A Preliminary Report. Cells 2024, 13: 522. PMID: 38534368, PMCID: PMC10969736, DOI: 10.3390/cells13060522.Peer-Reviewed Original ResearchConceptsMDA-MB-231Transcriptomic platformsGene expressionSpatially localized gene expressionMDA-MB-231 metastatic breast cancer cellsSpatial transcriptomics platformGenetic expression changesFracture healingMetastatic breast cancer cellsBreast cancer cellsImpaired fracture healingSingle-cell RNALocal gene expressionSpatial transcriptomic profilingFracture callus formationGenomic heterogeneitySequencing techniquesDisrupt bone homeostasisTranscriptome profilingIn situ hybridizationExpression changesSoft callusPathological fracturesWild-typeSpatial transcriptomics
2021
Inflammatory conversion of quiescent osteoblasts by metastatic breast cancer cells through pERK1/2 aggravates cancer-induced bone destruction
Back J, Nguyen MN, Li L, Lee S, Lee I, Chen F, Gillinov L, Chung YH, Alder KD, Kwon HK, Yu KE, Dussik CM, Hao Z, Flores MJ, Kim Y, Ibe IK, Munger AM, Seo SW, Lee FY. Inflammatory conversion of quiescent osteoblasts by metastatic breast cancer cells through pERK1/2 aggravates cancer-induced bone destruction. Bone Research 2021, 9: 43. PMID: 34588427, PMCID: PMC8481290, DOI: 10.1038/s41413-021-00158-w.Peer-Reviewed Original ResearchCancer-induced bone destructionBreast cancer cellsOsteolytic breast cancerBone homeostasisCancer growthCancer cellsBone destructionInflammatory osteolysisBreast cancerBreast cancer-induced bone destructionOsteolytic breast cancer metastasesPromising adjuvant therapyERK1/2 activationMEK1 expressionBreast cancer metastasisMetastatic breast cancer cellsActivation of pERK1/2Inflammatory conversionAdjuvant therapyPathological fracturesHuman pathological specimensBone microenvironmentPathological specimensSkeletal diseasePathway inhibitorInduction of interferon signaling and allograft inflammatory factor 1 in macrophages in a mouse model of breast cancer metastases
Zheng W, Zhao D, Zhang H, Chinnasamy P, Sibinga N, Pollard JW. Induction of interferon signaling and allograft inflammatory factor 1 in macrophages in a mouse model of breast cancer metastases. Wellcome Open Research 2021, 6: 52. PMID: 33824914, PMCID: PMC8008350, DOI: 10.12688/wellcomeopenres.16569.2.Peer-Reviewed Original ResearchGene expression programsDifferent gene expression profilesGene-targeted mouse modelsGene expression profilesFactor 1Interferon-responsive genesAllograft inflammatory factor-1Metastatic breast cancer cellsExpression programsInflammatory factor-1Functional validationExpression profilesActivated pathwaysMetastatic growthC57BL6/J backgroundBreast cancer cellsSignificant enrichmentFunctional relevanceInterferon SignalingResident alveolar macrophagesBreast cancer metastasisQRT-PCRStrong upregulationCancer metastasisHuman macrophagesInduction of interferon signaling and allograft inflammatory factor 1 in macrophages in a mouse model of breast cancer metastases
Zheng W, Zhao D, Zhang H, Chinnasamy P, Sibinga N, Pollard J. Induction of interferon signaling and allograft inflammatory factor 1 in macrophages in a mouse model of breast cancer metastases. Wellcome Open Research 2021, 6: 52. DOI: 10.12688/wellcomeopenres.16569.1.Peer-Reviewed Original ResearchGene expression programsDifferent gene expression profilesGene-targeted mouse modelsGene expression profilesFactor 1Interferon-responsive genesAllograft inflammatory factor-1Metastatic breast cancer cellsExpression programsInflammatory factor-1Functional validationExpression profilesActivated pathwaysMetastatic growthC57BL6/J backgroundBreast cancer cellsSignificant enrichmentFunctional relevanceInterferon SignalingResident alveolar macrophagesBreast cancer metastasisQRT-PCRStrong upregulationCancer metastasisHuman macrophages
2019
Breast Cancer Cells Transition from Mesenchymal to Amoeboid Migration in Tunable Three-Dimensional Silk–Collagen Hydrogels
Khoo AS, Valentin TM, Leggett SE, Bhaskar D, Bye EM, Benmelech S, Ip BC, Wong IY. Breast Cancer Cells Transition from Mesenchymal to Amoeboid Migration in Tunable Three-Dimensional Silk–Collagen Hydrogels. ACS Biomaterials Science & Engineering 2019, 5: 4341-4354. PMID: 31517039, PMCID: PMC6739834, DOI: 10.1021/acsbiomaterials.9b00519.Peer-Reviewed Original ResearchCell-matrix adhesionStrong cell-matrix adhesionMesenchymal morphologyCancer cell transitionCancer cellsCollagen I concentrationAmoeboid morphologyMetastatic breast cancer cellsCancer cell migrationCytoskeletal functionECM protein concentrationMigration phenotypeAmoeboid migrationCell transitionMesenchymal migrationBiochemical cuesBreast cancer cellsCell migrationExtracellular matrixRounded morphologyBiphasic dependencePlate formatCollagen ICellsBiomaterial platform
2012
Identification of NOG as a Specific Breast Cancer Bone Metastasis-supporting Gene* ♦
Tarragona M, Pavlovic M, Arnal-Estapé A, Urosevic J, Morales M, Guiu M, Planet E, González-Suárez E, Gomis R. Identification of NOG as a Specific Breast Cancer Bone Metastasis-supporting Gene* ♦. Journal Of Biological Chemistry 2012, 287: 21346-21355. PMID: 22547073, PMCID: PMC3375555, DOI: 10.1074/jbc.m112.355834.Peer-Reviewed Original ResearchConceptsBreast cancer cellsCancer cellsPrimary siteNOG expressionBone metastatic potentialBone metastatic lesionsMetastatic breast cancer cellsHuman breast cancer cellsAggressive cancer cellsBone relapseMetastatic lesionsPrimary tumorMetastatic nicheTumor cellsBone colonizationMetastatic potentialDistant organsMetastasisOsteoclast differentiationColonic functionBone degradationCell functionNOGBMP inhibitorsBone
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