2022
Targeted Discovery of Amantamide B, an Ion Channel Modulating Nonapeptide from a South China Sea Oscillatoria Cyanobacterium
Li T, Xi C, Yu Y, Wang N, Wang X, Iwasaki A, Fang F, Ding L, Li S, Zhang W, Yuan Y, Wang T, Yan X, He S, Cao Z, Naman C. Targeted Discovery of Amantamide B, an Ion Channel Modulating Nonapeptide from a South China Sea Oscillatoria Cyanobacterium. Journal Of Natural Products 2022, 85: 493-500. PMID: 34986303, DOI: 10.1021/acs.jnatprod.1c00983.Peer-Reviewed Original ResearchConceptsChiral-phase HPLC analysisChemical degradationConfigurational assignmentDereplication toolLC-MS/MSTargeted isolationU937 human histiocytic lymphoma cellsTargeted discoveryGPCR targetsFilamentous cyanobacteriumNotable cytotoxicityOrganic extractsHuman histiocytic lymphoma cellsHistiocytic lymphoma cellsCompoundsHuman T lymphoblastoid cellsCXCR7 agonistPrimary cultured neocortical neuronsMethyl esterLymphoblastoid cellsMoleculesT-lymphoblastoid cellsCyanobacteriumNonapeptide analoguesΜM
2018
Mapping and characterizing N6-methyladenine in eukaryotic genomes using single-molecule real-time sequencing
Zhu S, Beaulaurier J, Deikus G, Wu TP, Strahl M, Hao Z, Luo G, Gregory JA, Chess A, He C, Xiao A, Sebra R, Schadt EE, Fang G. Mapping and characterizing N6-methyladenine in eukaryotic genomes using single-molecule real-time sequencing. Genome Research 2018, 28: 1067-1078. PMID: 29764913, PMCID: PMC6028124, DOI: 10.1101/gr.231068.117.Peer-Reviewed Original ResearchConceptsSingle-molecule real-time sequencingEukaryotic genomesReal-time sequencingDAS eventsN6-methyladenineHuman lymphoblastoid cellsGenome-wide mapsSingle-nucleotide resolutionSingle-molecule resolutionLINE-1 elementsFull-length LINE-1 elementsGreen algaeProkaryotic genomesGenomeHigh-resolution mappingSequencing dataLymphoblastoid cellsSequencingEukaryotesProkaryotesMethylomeNovel formAlgaePromoterJoint analysis
2009
A Robust Approach to Identifying Tissue-Specific Gene Expression Regulatory Variants Using Personalized Human Induced Pluripotent Stem Cells
Lee JH, Park IH, Gao Y, Li JB, Li Z, Daley GQ, Zhang K, Church GM. A Robust Approach to Identifying Tissue-Specific Gene Expression Regulatory Variants Using Personalized Human Induced Pluripotent Stem Cells. PLOS Genetics 2009, 5: e1000718. PMID: 19911041, PMCID: PMC2766639, DOI: 10.1371/journal.pgen.1000718.Peer-Reviewed Original ResearchMeSH KeywordsAllelesCell DifferentiationCell LineCells, CulturedCluster AnalysisComputational BiologyDNA, ComplementaryFlow CytometryGene Expression RegulationHuman Genome ProjectHumansInduced Pluripotent Stem CellsNucleic Acid Amplification TechniquesOrgan SpecificityRegulatory Elements, TranscriptionalReproducibility of ResultsConceptsCis-regulatory variantsPluripotent stem cellsRegulatory polymorphismsGene expressionAllele-specific gene expressionIPS cellsPrimary fibroblastsCell typesAllele-specific expressionHigh-throughput sequencingStem cellsHuman iPS cellsSkin primary fibroblastsRegulatory variantsTissue-specific variantsX chromosomeDifferentiated derivativesExpression locusCell differentiationSpecific tissuesLymphoblastoid cellsDigital-RNAPadlock probesExpressionCells
2008
Mutation in EGFP Domain of LDL Receptor-Related Protein 6 Impairs Cellular LDL Clearance
Liu W, Mani S, Davis NR, Sarrafzadegan N, Kavathas PB, Mani A. Mutation in EGFP Domain of LDL Receptor-Related Protein 6 Impairs Cellular LDL Clearance. Circulation Research 2008, 103: 1280-1288. PMID: 18948618, PMCID: PMC3426315, DOI: 10.1161/circresaha.108.183863.Peer-Reviewed Original ResearchMeSH Keywords3T3 CellsAmino Acid SubstitutionAnimalsApolipoproteins BB-LymphocytesCell LineCell MembraneEndosomesGene Expression RegulationGenetic Carrier ScreeningGreen Fluorescent ProteinsHumansImmunohistochemistryLDL-Receptor Related ProteinsLipoproteins, LDLLow Density Lipoprotein Receptor-Related Protein-6LysosomesMetabolic Clearance RateMiceMice, KnockoutMicroscopy, FluorescenceMutationConceptsLDL clearanceMutation carriersWild-type receptorEarly-onset atherosclerosisLow-density lipoprotein uptakeWild-type littermatesLymphoblastoid cellsSplenic B cellsTotal LRP6LDL receptor-related protein 6Unaffected family membersApolipoprotein BLRP6 expressionMembrane expression levelsB cellsLipoprotein uptakeLDL uptakeCholesterol uptakeLDL receptorLow efficacyPlasma membrane expression levelsReceptorsLDL endocytosisMembrane expressionProtein 6
2006
A limited number of genes are involved in the differentiation of germinal center B cells
Nakayama Y, Stabach P, Maher SE, Mahajan MC, Masiar P, Liao C, Zhang X, Ye Z, Tuck D, Bothwell AL, Newburger PE, Weissman SM. A limited number of genes are involved in the differentiation of germinal center B cells. Journal Of Cellular Biochemistry 2006, 99: 1308-1325. PMID: 16795035, DOI: 10.1002/jcb.20952.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsB-LymphocytesCell DifferentiationCell LineChildChild, PreschoolDithiothreitolDNA-Binding ProteinsGene Expression ProfilingGerminal CenterHumansMolecular Sequence DataNuclear ProteinsOligonucleotide Array Sequence AnalysisPalatine TonsilProtein FoldingRegulatory Factor X Transcription FactorsTranscription FactorsTunicamycinX-Box Binding Protein 1ConceptsUnfolded protein responseX-box binding protein 1Interferon regulatory factor 4Protein 1B lymphocyte-induced maturation protein-1Transcription factor B lymphocyte-induced maturation protein-1Post-transcriptional changesBinding protein 1Maturation protein-1Mature B cellsDisulfide isomeraseTranscription factorsLevel of expressionPlasmacytoma cell lineProtein responseGene expressionRegulatory factor 4GenesGerminal center B cellsLymphoblastoid cellsLimited inductionCell linesB cellsFactor 4Germinal center centroblasts
2004
Deficiency of polycystin‐2 reduces Ca2+ channel activity and cell proliferation in ADPKD lymphoblastoid cells
Aguiari G, Banzi M, Gessi S, Cai Y, Zeggio E, Manzati E, Piva R, Lambertini E, Ferrari L, Peters DJ, Lanza F, Harris PC, Borea PA, Somlo S, del Senno L. Deficiency of polycystin‐2 reduces Ca2+ channel activity and cell proliferation in ADPKD lymphoblastoid cells. The FASEB Journal 2004, 18: 884-886. PMID: 15001556, DOI: 10.1096/fj.03-0687fje.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SubstitutionB-LymphocytesCalciumCalcium SignalingCell DivisionCell Line, TransformedCodon, NonsenseEndoplasmic ReticulumGentamicinsHumansIon TransportKidneyMembrane ProteinsMutation, MissenseOrgan SpecificityPlatelet Activating FactorPoint MutationPolycystic Kidney, Autosomal DominantProteinsRNA, MessengerSuppression, GeneticTRPP Cation ChannelsConceptsPlatelet-activating factorAutosomal dominant polycystic kidney disease patientsPolycystic kidney disease patientsKidney disease patientsCell proliferationPolycystin-2B lymphoblastoid cellsDisease patientsADPKD patientsB-LCLIntracellular Ca2PKD2 mutationsPC2 levelsKidney epitheliumPatientsChannel activityLymphoblastoid cellsAminoglycoside antibioticsKidney cellsImportant regulatorHEK293 cellsPKD2 geneFunction activityCa2PKD genes
1999
Mutations in autosomal dominant polycystic kidney disease 2 gene: Reduced expression of PKD2 protein in lymphoblastoid cells
Aguiari G, Manzati E, Penolazzi L, Micheletti F, Augello G, De Vitali E, Cappelli G, Cai Y, Reynolds D, Somlo S, Piva R, del Senno L. Mutations in autosomal dominant polycystic kidney disease 2 gene: Reduced expression of PKD2 protein in lymphoblastoid cells. American Journal Of Kidney Diseases 1999, 33: 880-885. PMID: 10213643, DOI: 10.1016/s0272-6386(99)70420-8.Peer-Reviewed Original ResearchConceptsPolycystic kidney disease 2 (PKD2) geneMembrane-spanning domainsIntegral membrane proteinsLymphoblastoid cellsFirst extracellular loopAutosomal dominant polycystic kidney diseasePKD2 proteinMembrane proteinsRestriction enzyme analysisCommon genetic diseaseLymphoblastoid cell linesProtein productsMutant allelesExtracellular loopWestern blot analysisPKD2 genePolymerase chain reactionGenetic diseasesNormal proteinAmino acidsMessenger RNA levelsNonsense mutationFrameshift mutationGenesProtein
1998
Human endothelial cells induce and regulate cytolytic T cell differentiation.
Biedermann B, Pober J. Human endothelial cells induce and regulate cytolytic T cell differentiation. The Journal Of Immunology 1998, 161: 4679-87. PMID: 9794397, DOI: 10.4049/jimmunol.161.9.4679.Peer-Reviewed Original ResearchConceptsB lymphoblastoid cellsEndothelial cellsHuman endothelial cellsSame donorT cellsProliferation of CD8IFN-gamma secretionCultured human endothelial cellsT cell differentiationT cell growthAllogeneic CD8CTL responsesPerforin expressionCTL differentiationPresence of ECT lymphocytesAllospecific CTLCD8CTLEfficient stimulatorsLymphoblastoid cellsCell numberReproducible frequencyCell growthInput cell number
1995
Different MHC class I alleles compete for presentation of overlapping viral epitopes
Tussey L, Rowland-Jones S, Zheng T, Androlewicz M, Cresswell P, Frelinger J, McMichael A. Different MHC class I alleles compete for presentation of overlapping viral epitopes. Immunity 1995, 3: 65-77. PMID: 7542549, DOI: 10.1016/1074-7613(95)90159-0.Peer-Reviewed Original Research
1994
Epstein-Barr viral nuclear antigen 1 antisense oligodeoxynucleotide inhibits proliferation of Epstein-Barr virus-immortalized B cells.
Roth G, Curiel T, Lacy J. Epstein-Barr viral nuclear antigen 1 antisense oligodeoxynucleotide inhibits proliferation of Epstein-Barr virus-immortalized B cells. Blood 1994, 84: 582-7. PMID: 8025284, DOI: 10.1182/blood.v84.2.582.bloodjournal842582.Peer-Reviewed Original ResearchConceptsEBNA-1EBNA-1 proteinAntisense oligodeoxynucleotidesEpstein-Barr virus-immortalized B cellsEpstein-Barr virus nuclear antigen 1EBV-negative cellsLatent viral proteinsNuclear antigen 1Potential therapeutic implicationsLymphoblastoid cellsEpisomal viral genomesUnmodified antisense oligodeoxynucleotidesNeoplastic diseaseTherapeutic implicationsB cellsInhibited proliferationEBVEffect of antisenseAntigen 1Antiproliferative effectsProtein expressionUntreated cellsImmortalized cellsViral proteinsProliferationEpstein-Barr Viral Nuclear Antigen 1 Antisense Oligodeoxynucleotide Inhibits Proliferation of Epstein-Barr Virus-Immortalized B Cells
Roth G, Curiel T, Lacy J. Epstein-Barr Viral Nuclear Antigen 1 Antisense Oligodeoxynucleotide Inhibits Proliferation of Epstein-Barr Virus-Immortalized B Cells. Blood 1994, 84: 582-587. DOI: 10.1182/blood.v84.2.582.582.Peer-Reviewed Original ResearchEBNA-1EBNA-1 proteinAntisense oligodeoxynucleotidesEpstein-Barr virus nuclear antigen 1EBV-negative cellsEpstein-Barr virusLatent viral proteinsNuclear antigen 1Potential therapeutic implicationsLymphoblastoid cellsImmortalized B cellsEpisomal viral genomesUnmodified antisense oligodeoxynucleotidesNeoplastic diseaseTherapeutic implicationsB cellsInhibited proliferationInhibits proliferationEBVEffect of antisenseAntigen 1Antiproliferative effectsProtein expressionUntreated cellsImmortalized cells
1991
Conversion of a human B cell lymphoma line by Epstein-Barr virus is associated with increased tyrosine phosphorylation of a 50 kilodalton cytosolic protein
Lacy J, Bartiss A, Coleman D. Conversion of a human B cell lymphoma line by Epstein-Barr virus is associated with increased tyrosine phosphorylation of a 50 kilodalton cytosolic protein. Virus Research 1991, 20: 85-96. PMID: 1656626, DOI: 10.1016/0168-1702(91)90063-2.Peer-Reviewed Original ResearchConceptsEpstein-Barr virusB cellsHuman B cellsB-cell lymphoma linePresence of EBVLymphoma linesProtein tyrosine phosphorylationEBV infectionHuman B cell lymphoma lineTyrosine phosphorylationB lymphocytesImmortalized lymphoblastoid cellsBiochemical changesLymphoblastoid cellsInfectionEndogenous tyrosine kinasesContinuous proliferationCytosolic proteinsTyrosine kinaseVirusCellsPhosphorylationPhosphotyrosine antibodiesLymphocytesProtein
1986
Expression of differentiation antigens by hybrids of human lymphoblastoid cells
Howell D, Salter R, Koppelman B, Cresswell P. Expression of differentiation antigens by hybrids of human lymphoblastoid cells. Human Immunology 1986, 17: 443-455. PMID: 3098709, DOI: 10.1016/0198-8859(86)90303-4.Peer-Reviewed Original ResearchConceptsParent cell lineCell linesClass IClass II antigensB lymphocyte antigensClass II histocompatibility antigensLymphocyte differentiation markersT-lymphoblastoid cell lineII antigensHistocompatibility antigensLymphocyte antigenDifferentiation antigensLymphoblastoid cell linesParental antigensHNK-1AntigenMonoclonal antibodiesHuman lymphoblastoid cellsT-LCLDifferentiation markersLymphoblastoid cellsCurrent study
1978
Phosphorylation in vivo and in vitro of human histocompatibility antigens (HLA-A and HLA-B) in the carboxy-terminal intracellular domain.
Pober JS, Guild BC, Strominger JL. Phosphorylation in vivo and in vitro of human histocompatibility antigens (HLA-A and HLA-B) in the carboxy-terminal intracellular domain. Proceedings Of The National Academy Of Sciences Of The United States Of America 1978, 75: 6002-6006. PMID: 282620, PMCID: PMC393105, DOI: 10.1073/pnas.75.12.6002.Peer-Reviewed Original Research
1972
Epstein-Barr Virus: Transformation, Cytopathic Changes, and Viral Antigens in Squirrel Monkey and Marmoset Leukocytes
Miller G, Shope T, Lisco H, Stitt D, Lipman M. Epstein-Barr Virus: Transformation, Cytopathic Changes, and Viral Antigens in Squirrel Monkey and Marmoset Leukocytes. Proceedings Of The National Academy Of Sciences Of The United States Of America 1972, 69: 383-387. PMID: 4333982, PMCID: PMC426463, DOI: 10.1073/pnas.69.2.383.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigen-Antibody ReactionsAntigens, ViralCattleCell LineCell Transformation, NeoplasticCells, CulturedChromosomesComplement Fixation TestsCytopathogenic Effect, ViralFetusFibroblastsFluorescent Antibody TechniqueHaplorhiniHerpesviridaeHerpesvirus 4, HumanHumansImmune SeraKidneyLeukocytesPathologyPlacentaConceptsEpstein-Barr virus antigensEpstein-Barr virusVirus antigenGiant cellsMultinucleate giant cellsViral antigensBlood leukocytesCytopathic changesNew World primatesInfectious virusIntranuclear inclusionsSquirrel monkeysAntigenLymphoblastoid cellsVirusLeukocytesWorld primatesSimian cellsCellsLarge proportionMultinucleate cells
This site is protected by hCaptcha and its Privacy Policy and Terms of Service apply