2024
Direct cardiac reprogramming via combined CRISPRa-mediated endogenous Gata4 activation and exogenous Mef2c and Tbx5 expression
Huang P, Xu J, Keepers B, Xie Y, Near D, Xu Y, Hua J, Spurlock B, Ricketts S, Liu J, Wang L, Qian L. Direct cardiac reprogramming via combined CRISPRa-mediated endogenous Gata4 activation and exogenous Mef2c and Tbx5 expression. Molecular Therapy - Nucleic Acids 2024, 35: 102390. PMID: 39720701, PMCID: PMC11666955, DOI: 10.1016/j.omtn.2024.102390.Peer-Reviewed Original ResearchActivity of endogenous factorsDirect cardiac reprogrammingInduced cardiomyocytesCardiac reprogrammingExpression of cardiac transcription factorsCardiac transcription factorsTranscription factorsReprogramming of fibroblastsSingle-guide RNAViral toxicityViral vectorsExogenous transcription factorsImmune responseEpigenetic barriersGATA4 activityRegenerative purposesClinical applicationEndogenous factorsEndogenous transcription factorsGenomic mutationsExogenous expressionReprogrammingFibroblastsEndogenous gene expressionHuman fibroblasts
2023
Translational landscape of direct cardiac reprogramming reveals a role of Ybx1 in repressing cardiac fate acquisition
Xie Y, Wang Q, Yang Y, Near D, Wang H, Colon M, Nguyen C, Slattery C, Keepers B, Farber G, Wang T, Lee S, Ian Shih Y, Liu J, Qian L. Translational landscape of direct cardiac reprogramming reveals a role of Ybx1 in repressing cardiac fate acquisition. Nature Cardiovascular Research 2023, 2: 1060-1077. PMID: 38524149, PMCID: PMC10959502, DOI: 10.1038/s44161-023-00344-5.Peer-Reviewed Original ResearchTranslational regulationTranslational landscapeLayer of regulatory mechanismLoss-of-function screensY-box binding protein 1ICM reprogrammingBinding protein 1Induced cardiomyocytes generationFate acquisitionInduced cardiomyocytesEpigenetic mechanismsTranscriptome profilingTranslational levelY-boxRegulatory mechanismsYBX1Reprogramming of fibroblastsReprogrammingDirect reprogramming of fibroblastsCardiac reprogrammingProtein 1Mouse model of myocardial infarctionReduced scar sizeDirect reprogrammingModel of myocardial infarction
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